Abstract
Optimal Conditions for cutting thin sections from fresh-frozen tissue have been described in terms of a set of optimal temperatures for the tissue block, microtome chamber, and knife. A model for the cutting process is proposed which postulates a zone of "micro-melting" at the edge of the knife. The behavior of the freezing microtome is interpreted in terms of this model for a variety of conditions.
Dates
Type | When |
---|---|
Created | 14 years, 4 months ago (April 1, 2011, 12:43 a.m.) |
Deposited | 5 months, 2 weeks ago (March 3, 2025, 7:30 a.m.) |
Indexed | 5 months, 2 weeks ago (March 4, 2025, 12:39 a.m.) |
Issued | 68 years, 7 months ago (Jan. 1, 1957) |
Published | 68 years, 7 months ago (Jan. 1, 1957) |
Published Online | 68 years, 7 months ago (Jan. 1, 1957) |
Published Print | 68 years, 7 months ago (Jan. 1, 1957) |
@article{THORNBURG_1957, title={AN ANALYSIS OF FROZEN SECTION TECHNIQUES: I. SECTIONING OF FRESH-FROZEN TISSUE}, volume={5}, ISSN={1551-5044}, url={http://dx.doi.org/10.1177/5.1.47}, DOI={10.1177/5.1.47}, number={1}, journal={Journal of Histochemistry & Cytochemistry}, publisher={SAGE Publications}, author={THORNBURG, WAYNE and MENGERS, PAUL E.}, year={1957}, month=jan, pages={47–52} }