Abstract
Specific DNA staining for electron microscopic observation is simplified by a shorter synthesis of the staining reagent. The new, more reliable reagent, osmium ammine-B, is stable for more than a year, dissolves completely in water, and does not require reoptimization of staining conditions for every batch, yet reproducibly gives strong contrast to DNA-containing structures.
Dates
Type | When |
---|---|
Created | 14 years, 4 months ago (April 1, 2011, 2:04 a.m.) |
Deposited | 5 months, 3 weeks ago (March 2, 2025, 10:22 p.m.) |
Indexed | 4 months, 2 weeks ago (April 8, 2025, 10:24 a.m.) |
Issued | 36 years, 5 months ago (March 1, 1989) |
Published | 36 years, 5 months ago (March 1, 1989) |
Published Online | 36 years, 5 months ago (March 1, 1989) |
Published Print | 36 years, 5 months ago (March 1, 1989) |
@article{Olins_1989, title={Synthesis of a more stable osmium ammine electron-dense DNA stain.}, volume={37}, ISSN={1551-5044}, url={http://dx.doi.org/10.1177/37.3.2465337}, DOI={10.1177/37.3.2465337}, number={3}, journal={Journal of Histochemistry & Cytochemistry}, publisher={SAGE Publications}, author={Olins, A L and Moyer, B A and Kim, S H and Allison, D P}, year={1989}, month=mar, pages={395–398} }