DOI: 10.1177/35.11.3116075. Effect of tissue fixation on anti-bromodeoxyuridine immunohistochemistry.

Effect of tissue fixation on anti-bromodeoxyuridine immunohistochemistry.

10.1177/35.11.3116075

Crossref journal-article

SAGE Publications

Journal of Histochemistry & Cytochemistry (179)

Abstract

We investigated the influence of various fixatives on monoclonal anti-BrdUrd antibody binding of BrdUrd-substituted DNA in tissue sections of routinely processed mouse small intestine after in vivo administration of BrdUrd. For denaturing fixatives such as ethanol or Carnoy's fluid, a standard denaturation protocol showed specific crypt cell labeling. With cross-linking agents such as formalin and glutaraldehyde, a remarkable increase in staining intensity was obtained after tissue digestion with pepsin before acid denaturation. The optimal pepsin concentration was determined for maximal immunoreactivity combined with acceptable morphology.

Bibliography

Schutte, B., Reynders, M. M., Bosman, F. T., & Blijham, G. H. (1987). Effect of tissue fixation on anti-bromodeoxyuridine immunohistochemistry. Journal of Histochemistry & Cytochemistry, 35(11), 1343â1345.

Authors 4

B Schutte (first)
M M Reynders (additional)
F T Bosman (additional)
G H Blijham (additional)

References 0 Referenced 108

None

Dates

Type	When
Created	14 years, 4 months ago (April 1, 2011, 2:04 a.m.)
Deposited	7 months ago (Jan. 28, 2025, 10:44 a.m.)
Indexed	3 months, 1 week ago (May 19, 2025, 8:08 a.m.)
Issued	37 years, 9 months ago (Nov. 1, 1987)
Published	37 years, 9 months ago (Nov. 1, 1987)
Published Online	37 years, 9 months ago (Nov. 1, 1987)
Published Print	37 years, 9 months ago (Nov. 1, 1987)

Funders 0

None

BibTeX

@article{Schutte_1987, title={Effect of tissue fixation on anti-bromodeoxyuridine immunohistochemistry.}, volume={35}, ISSN={1551-5044}, url={http://dx.doi.org/10.1177/35.11.3116075}, DOI={10.1177/35.11.3116075}, number={11}, journal={Journal of Histochemistry & Cytochemistry}, publisher={SAGE Publications}, author={Schutte, B and Reynders, M M and Bosman, F T and Blijham, G H}, year={1987}, month=nov, pages={1343–1345} }

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