DOI: 10.1177/33.1.2578146. An improved method to determine cell viability by simultaneous staining with fluorescein diacetate-propidium iodide.

An improved method to determine cell viability by simultaneous staining with fluorescein diacetate-propidium iodide.

10.1177/33.1.2578146

Crossref journal-article

SAGE Publications

Journal of Histochemistry & Cytochemistry (179)

Abstract

A rapid, simultaneous double-staining procedure using fluorescein diacetate (FDA) and propidium iodide (PI) is described for use in the determination of cell viability in cell suspension. Air-dried slide preparations can be made from the cell suspensions so that an accurate estimate of the viability of the cells in the original suspension can be made up to 1 week later. Viable cells fluoresce bright green, while nonviable cells are bright red. Furthermore, when FDA-PI staining is compared to trypan blue dye exclusion as a method to determine cell viability, FDA-PI is found to be more consistent over prolonged periods of exposure to the dyes. Therefore, double staining with FDA-PI is a rapid, convenient, and reliable method to determine cell viability.

Bibliography

Jones, K. H., & Senft, J. A. (1985). An improved method to determine cell viability by simultaneous staining with fluorescein diacetate-propidium iodide. Journal of Histochemistry & Cytochemistry, 33(1), 77â79.

Authors 2

K H Jones (first)
J A Senft (additional)

References 0 Referenced 893

None

Dates

Type	When
Created	14 years, 5 months ago (April 1, 2011, 2:04 a.m.)
Deposited	6 months ago (March 2, 2025, 6:59 a.m.)
Indexed	2 days, 5 hours ago (Sept. 4, 2025, 9:44 a.m.)
Issued	40 years, 8 months ago (Jan. 1, 1985)
Published	40 years, 8 months ago (Jan. 1, 1985)
Published Online	40 years, 8 months ago (Jan. 1, 1985)
Published Print	40 years, 8 months ago (Jan. 1, 1985)

Funders 0

None

BibTeX

@article{Jones_1985, title={An improved method to determine cell viability by simultaneous staining with fluorescein diacetate-propidium iodide.}, volume={33}, ISSN={1551-5044}, url={http://dx.doi.org/10.1177/33.1.2578146}, DOI={10.1177/33.1.2578146}, number={1}, journal={Journal of Histochemistry & Cytochemistry}, publisher={SAGE Publications}, author={Jones, K H and Senft, J A}, year={1985}, month=jan, pages={77–79} }

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