Abstract
The effects of p-phenylenediamine (PD) on the extinction and intensity of fluorescence and on antigen binding in immunofluorescence microscopy was investigated. Visual and photometric comparison of tissue sections reacted sequentially with polyclonal or monoclonal antisera and appropriate fluorochromes and mounted with and without PD indicated that PD both prolonged and intensified fluorescence without evident effect on antibody binding.
Dates
Type | When |
---|---|
Created | 14 years, 5 months ago (April 1, 2011, 2:04 a.m.) |
Deposited | 7 months, 1 week ago (Jan. 27, 2025, 10 p.m.) |
Indexed | 2 days, 3 hours ago (Sept. 4, 2025, 9:58 a.m.) |
Issued | 42 years, 3 months ago (June 1, 1983) |
Published | 42 years, 3 months ago (June 1, 1983) |
Published Online | 42 years, 3 months ago (June 1, 1983) |
Published Print | 42 years, 3 months ago (June 1, 1983) |
@article{Platt_1983, title={Retardation of fading and enhancement of intensity of immunofluorescence by p-phenylenediamine.}, volume={31}, ISSN={1551-5044}, url={http://dx.doi.org/10.1177/31.6.6341464}, DOI={10.1177/31.6.6341464}, number={6}, journal={Journal of Histochemistry & Cytochemistry}, publisher={SAGE Publications}, author={Platt, J L and Michael, A F}, year={1983}, month=jun, pages={840–842} }