DOI: 10.1177/29.10.7028859. Suppression of endogenous avidin-binding activity in tissues and its relevance to biotin-avidin detection systems.

Suppression of endogenous avidin-binding activity in tissues and its relevance to biotin-avidin detection systems.

10.1177/29.10.7028859

Crossref journal-article

SAGE Publications

Journal of Histochemistry & Cytochemistry (179)

Abstract

As biotin-avidin systems continue to be developed for applications involving single cells, cell suspensions, and especially tissue sections, the need arises for a method of blocking endogenous avidin-binding activity. One such method is described and its proposed mechanism is discussed. Utilizing this method, endogenous avidin-binding activity was detected and suppressed in selected human and murine tissues, thus facilitating the interpretation of specific immunohistochemical staining utilizing hybridoma monoclonal antibodies in a biotin-avidin-horseradish peroxidase detection system.

Bibliography

Wood, G. S., & Warnke, R. (1981). Suppression of endogenous avidin-binding activity in tissues and its relevance to biotin-avidin detection systems. Journal of Histochemistry & Cytochemistry, 29(10), 1196â1204.

Authors 2

G S Wood (first)
R Warnke (additional)

References 0 Referenced 384

None

Dates

Type	When
Created	14 years, 4 months ago (April 1, 2011, 2:04 a.m.)
Deposited	5 months, 2 weeks ago (March 4, 2025, 12:33 a.m.)
Indexed	3 weeks, 3 days ago (July 30, 2025, 11:14 a.m.)
Issued	43 years, 10 months ago (Oct. 1, 1981)
Published	43 years, 10 months ago (Oct. 1, 1981)
Published Online	43 years, 10 months ago (Oct. 1, 1981)
Published Print	43 years, 10 months ago (Oct. 1, 1981)

Funders 0

None

BibTeX

@article{Wood_1981, title={Suppression of endogenous avidin-binding activity in tissues and its relevance to biotin-avidin detection systems.}, volume={29}, ISSN={1551-5044}, url={http://dx.doi.org/10.1177/29.10.7028859}, DOI={10.1177/29.10.7028859}, number={10}, journal={Journal of Histochemistry & Cytochemistry}, publisher={SAGE Publications}, author={Wood, G S and Warnke, R}, year={1981}, month=oct, pages={1196–1204} }

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