DOI: 10.1177/28.9.6997370. Intracellular localization of fibronectin by immunoelectron microscopy.

Intracellular localization of fibronectin by immunoelectron microscopy.

10.1177/28.9.6997370

Crossref journal-article

SAGE Publications

Journal of Histochemistry & Cytochemistry (179)

Abstract

We have localized fibronectin, a major extracellular glycoprotein of cultured fibroblasts, in chick embryo fibroblasts at the ultrastructural level using affinity-purified antibodies to fibronectin. The use of a ferritin bridge procedure permitted quantitation of localization in various organelles. These results provide the first intracellular ultrastructural localization of fibronectin. Extracellular labeling was confined to aggregates and fibrils, with little or no labeling of the plasma membrane. The principal sites of intracellular localization were the rough endoplasmic reticulum and the Golgi apparatus. Treatment of cells with the protein synthesis inhibitors cycloheximide and pactamycin reduced fibronectin localization in the endoplasmic reticulum to 50% of normal levels. Removal of cycloheximide permitted recovery of labeling to 85% of control levels in the endoplasmic reticulum. Similar, but much reduced, changes also occurred in the Golgi apparatus.

Bibliography

Yamada, S. S., Yamada, K. M., & Willingham, M. C. (1980). Intracellular localization of fibronectin by immunoelectron microscopy. Journal of Histochemistry & Cytochemistry, 28(9), 953â960.

Authors 3

S S Yamada (first)
K M Yamada (additional)
M C Willingham (additional)

References 0 Referenced 31

None

Dates

Type	When
Created	14 years, 4 months ago (April 1, 2011, 2:04 a.m.)
Deposited	5 months, 4 weeks ago (March 3, 2025, 4:26 p.m.)
Indexed	5 months, 3 weeks ago (March 4, 2025, 12:57 a.m.)
Issued	44 years, 11 months ago (Sept. 1, 1980)
Published	44 years, 11 months ago (Sept. 1, 1980)
Published Online	44 years, 11 months ago (Sept. 1, 1980)
Published Print	44 years, 11 months ago (Sept. 1, 1980)

Funders 0

None

BibTeX

@article{Yamada_1980, title={Intracellular localization of fibronectin by immunoelectron microscopy.}, volume={28}, ISSN={1551-5044}, url={http://dx.doi.org/10.1177/28.9.6997370}, DOI={10.1177/28.9.6997370}, number={9}, journal={Journal of Histochemistry & Cytochemistry}, publisher={SAGE Publications}, author={Yamada, S S and Yamada, K M and Willingham, M C}, year={1980}, month=sep, pages={953–960} }

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