Abstract
Acidified 2,2-dimethoxypropane was used to chemically dehydrate biologic tissues for examination in the electron microscope. The ultrastructural integrities of single-celled algae, plant tissues (cotyledon, root, leaf) and animal tissues (liver, pancreas, muscle, cartilage) were maintained. Our technique was simpler and quicker than physically exchanging water for organic solvents (e.g., acetone, ethanol) as generally performed in microscopy.
Dates
Type | When |
---|---|
Created | 14 years, 4 months ago (April 1, 2011, 1:19 a.m.) |
Deposited | 5 months, 3 weeks ago (March 4, 2025, 3:02 a.m.) |
Indexed | 5 months, 3 weeks ago (March 5, 2025, 12:26 a.m.) |
Issued | 50 years, 6 months ago (Feb. 1, 1975) |
Published | 50 years, 6 months ago (Feb. 1, 1975) |
Published Online | 50 years, 6 months ago (Feb. 1, 1975) |
Published Print | 50 years, 6 months ago (Feb. 1, 1975) |
@article{Muller_1975, title={Rapid chemical dehydration of samples for electron microscopic examinations.}, volume={23}, ISSN={1551-5044}, url={http://dx.doi.org/10.1177/23.2.1117127}, DOI={10.1177/23.2.1117127}, number={2}, journal={Journal of Histochemistry & Cytochemistry}, publisher={SAGE Publications}, author={Muller, L L and Jacks, T J}, year={1975}, month=feb, pages={107–110} }