DOI: 10.1177/154405910308201209. Relative Levels of mRNA Encoding Enamel Proteins in Enamel Organ Epithelia and Odontoblasts

Relative Levels of mRNA Encoding Enamel Proteins in Enamel Organ Epithelia and Odontoblasts

10.1177/154405910308201209

Crossref journal-article

SAGE Publications

Journal of Dental Research (179)

Abstract

Amelogenin, enamelin, sheathlin (ameloblastin/ amelin), enamelysin (MMP-20), and KLK4 (EMSP-1) are the major structural proteins and proteinases in developing tooth enamel. Recently, odontoblasts were reported to express amelogenin, the most abundant enamel protein. In this study, we hypothesized that odontoblasts express all enamel proteins and proteases, and we measured their relative mRNA levels in enamel organ epithelia and odontoblasts associated with porcine secretory- and maturation-stage enamel by RT-PCR, using a LightCycler instrument. The results showed that amelogenin mRNA in secretory-stage EOE is 320-fold higher than in odontoblasts beneath secretory-stage enamel, and over 20,000-fold higher than in odontoblasts under maturation-stage enamel. Similar results were obtained for enamelin and sheathlin. Enamelysin mRNA levels were equivalent in these two tissues, while KLK4 mRNA was higher in odontoblasts than in secretory-stage EOE. These results support the conclusion that odontoblasts are involved in the formation of the enamel layer adjacent to enamel-dentin junction.

Bibliography

Nagano, T., Oida, S., Ando, H., Gomi, K., Arai, T., & Fukae, M. (2003). Relative Levels of mRNA Encoding Enamel Proteins in Enamel Organ Epithelia and Odontoblasts. Journal of Dental Research, 82(12), 982â986.

Authors 6

T. Nagano (first)
S. Oida (additional)
H. Ando (additional)
K. Gomi (additional)
T. Arai (additional)
M. Fukae (additional)

References 22 Referenced 60

10.1016/S0378-1119(96)00525-2
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10.1006/geno.1999.5990
10.1002/jbmr.5650110703
10.1007/BF02555263
10.3109/03008209809023918
Fukae M, Tanabe T, Shimizu M -1977- Proteolytic enzyme activity in porcine immature enamel. Tsurumi Shigaku 3:15–17.
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10.1007/s007740170026
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10.1074/jbc.271.8.4431
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Shimizu M, Fukae M -1983- Enamel proteins. In: Mechanisms of tooth enamel formation. Suga S, editor. Tokyo: Quintessence Publishing Co. Inc., pp. 125-141.
10.1177/00220345980770020601
10.1016/S0021-9258(18)43458-8
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Dates

Type	When
Created	16 years, 7 months ago (Jan. 8, 2009, 7:39 p.m.)
Deposited	6 months, 1 week ago (Feb. 28, 2025, 11:21 a.m.)
Indexed	3 days, 11 hours ago (Sept. 3, 2025, 6:05 a.m.)
Issued	21 years, 9 months ago (Dec. 1, 2003)
Published	21 years, 9 months ago (Dec. 1, 2003)
Published Online	21 years, 9 months ago (Dec. 1, 2003)
Published Print	21 years, 9 months ago (Dec. 1, 2003)

Funders 0

None

BibTeX

@article{Nagano_2003, title={Relative Levels of mRNA Encoding Enamel Proteins in Enamel Organ Epithelia and Odontoblasts}, volume={82}, ISSN={1544-0591}, url={http://dx.doi.org/10.1177/154405910308201209}, DOI={10.1177/154405910308201209}, number={12}, journal={Journal of Dental Research}, publisher={SAGE Publications}, author={Nagano, T. and Oida, S. and Ando, H. and Gomi, K. and Arai, T. and Fukae, M.}, year={2003}, month=dec, pages={982–986} }

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