DOI: 10.1177/15.4.243. FREEZE-DRYING OF SMALL TISSUE SAMPLES AND THIN FROZEN SECTIONS BELOW –60°C A SIMPLE METHOD OF CRYOSORPTION PUMPING

FREEZE-DRYING OF SMALL TISSUE SAMPLES AND THIN FROZEN SECTIONS BELOW –60°C A SIMPLE METHOD OF CRYOSORPTION PUMPING

10.1177/15.4.243

Crossref journal-article

SAGE Publications

Journal of Histochemistry & Cytochemistry (179)

Abstract

Freeze-drying rates for three tissues have been determined by direct measurement of weight loss in a closed system, using an electrobalance. The half-drying times (T/2) at approximately 1 x 10–5 mm Hg with Dry Ice slush as coolant were determined for brain, kidney and liver and found to be 100, 50 and 30 hr, respectively, for 10-mg samples of tissue. A method for the freeze-drying of frozen sections in which the temperature of the tissue is maintained below –60°C throughout sectioning and drying is discussed. A simple portable, all glass, freeze-drying system utilizing cryosorption pumping is presented.

Bibliography

STUMPF, W. E., & ROTH, L. J. (1967). FREEZE-DRYING OF SMALL TISSUE SAMPLES AND THIN FROZEN SECTIONS BELOW â60Â°C A SIMPLE METHOD OF CRYOSORPTION PUMPING. Journal of Histochemistry & Cytochemistry, 15(4), 243â251.

Authors 2

WALTER E. STUMPF (first)
LLOYD J. ROTH (additional)

References 0 Referenced 71

None

Dates

Type	When
Created	14 years, 4 months ago (April 1, 2011, 12:54 a.m.)
Deposited	5 months, 4 weeks ago (Feb. 28, 2025, 12:05 p.m.)
Indexed	5 months, 3 weeks ago (March 1, 2025, 12:42 a.m.)
Issued	58 years, 4 months ago (April 1, 1967)
Published	58 years, 4 months ago (April 1, 1967)
Published Online	58 years, 4 months ago (April 1, 1967)
Published Print	58 years, 4 months ago (April 1, 1967)

Funders 0

None

BibTeX

@article{STUMPF_1967, title={FREEZE-DRYING OF SMALL TISSUE SAMPLES AND THIN FROZEN SECTIONS BELOW –60°C A SIMPLE METHOD OF CRYOSORPTION PUMPING}, volume={15}, ISSN={1551-5044}, url={http://dx.doi.org/10.1177/15.4.243}, DOI={10.1177/15.4.243}, number={4}, journal={Journal of Histochemistry & Cytochemistry}, publisher={SAGE Publications}, author={STUMPF, WALTER E. and ROTH, LLOYD J.}, year={1967}, month=apr, pages={243–251} }

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