DOI: 10.1177/108705710200700310. A Homogeneous Caspase-3 Activity Assay Using HTRF® Technology

A Homogeneous Caspase-3 Activity Assay Using HTRF® Technology

10.1177/108705710200700310

Crossref journal-article

Elsevier BV

SLAS Discovery (78)

Bibliography

PrÃ©audat, M., Ouled-Diaf, J., Alpha-Bazin, B., Mathis, G., Mitsugi, T., Aono, Y., Takahashi, K., & Takemoto, H. (2002). A Homogeneous Caspase-3 Activity Assay Using HTRFÂ® Technology. SLAS Discovery, 7(3), 267â274.

Authors 8

M. Préaudat (first)
J. Ouled-Diaf (additional)
B. Alpha-Bazin (additional)
G. Mathis (additional)
T. Mitsugi (additional)
Y. Aono (additional)
K. Takahashi (additional)
H. Takemoto (additional)

References 18 Referenced 32

10.1038/376037a0 / Nature / Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis by Nicholson (1995)
10.1084/jem.183.5.1957 / J Exp Med / Apopain/CPP32 cleaves proteins that are essential for cellular repair: a fundamental principle of apoptotic death by Casciola-Rosen (1996)
Karvinen J, Andersson K, Hurskainen P, et al: Sensitive homogeneous protease assay based on time resolved fluorometry. Poster presented at the 6th Annual Conference and Exhibition of the Society for Biomolecular Screening, September 2000, Vancouver, British Columbia, Canada.
10.1038/sj.cdd.4400769 / Cell Growth Differ / Quantitative analysis of fluorescent caspase substrate cleavage in intact cells and identification of novel inhibitors of apoptosis by Tawa (2001)
10.1177/108705719600100407 / J Biomol Screen / Use of a novel homogeneous fluorescent technology in high throughput screening by Kolb (1996)
10.1177/108705719800300205 / J Biomol Screen / Development of a CD28/CD86 (B7-2) binding assay for high throughput screening by homogeneous time-resolved fluorescence by Mellor (1998)
10.1002/anie.198702661 / Angew Chem / Energy transfer luminescence of europium(III) and terbium(III) cryptates of macrobicyclic polypyridine ligands by Alpha (1987)
10.1093/clinchem/39.9.1953 / Clin Chem / Rare earth cryptates and homogeneous fluoroimmunoassays with human sera by Mathis (1993)
10.1016/S1386-1425(01)00493-0 / Spectrochim Acta A Mol Biomol Spectrosc / Homogeneous time resolved fluorescence energy transfer using rare earth cryptates as a tool for probing molecular interactions in biology by Bazin (2001)
Mathis G, Preaudat M, Trinquet E, et al: A new homogeneous method using rare earth cryptate and amplification by energy transfer for the characterization of the FOS and Jun leucine zipper peptides dimerization. In Proceedings of H.T.S. for Drug Development meeting, 1994, Philadelphia, PA.
Park YW, Cummings R, Hermes JD: Proximity high throughput kinase assay. Presented at the 3rd Annual Conference and Exhibition of the Society for Biomolecular Screening, September 1997, San Diego, CA.
10.1038/sj.onc.1203554 / Oncogene / Sensitization to CD95 ligand-induced apoptosis in human glioma cells by hyperthermia involves enhanced cytochrome C release by Hermisson (2000)
10.1126/science.281.5381.1312 / Science / Caspase: enemies within by Thornberry (1998)
Mabile M, Mathis G, Jolu EJP, et al: Method for measuring the luminescence in a luminescent assay. Patent WO 9213264; US 5527684, 1991.
10.1038/380723a0 / Nature / Sequential activation of ICE-like and CPP32-like protease during Fas-mediated apoptosis by Enari (1996)
10.1006/bbrc.1997.7370 / Biochem Biophys Res Common / Recombinant caspase-3 expressed in Pichia pastoris is fully activated and kinetically indistinguishable from the native enzyme by Sun (1997)
10.1177/108705710000500409 / J Biomol Screen / High throughput screening for human interferon-y production inhibitor using homogeneous time resolved fluorescence by Enomoto (2000)
10.2174/1386207302666220204200224 / Comb Chem High Throughput Screen / Application of homogeneous time resolved fluorescence (HTRF®) to monitor polyubiquitination of wild type p-53 by Yabuki (1999)

Dates

Type	When
Created	20 years, 3 months ago (May 17, 2005, 12:43 p.m.)
Deposited	2 years, 9 months ago (Nov. 19, 2022, 1:39 p.m.)
Indexed	4 months, 3 weeks ago (April 2, 2025, 10:02 a.m.)
Issued	23 years, 2 months ago (June 1, 2002)
Published	23 years, 2 months ago (June 1, 2002)
Published Print	23 years, 2 months ago (June 1, 2002)

Funders 0

None

BibTeX

@article{Pr_audat_2002, title={A Homogeneous Caspase-3 Activity Assay Using HTRF® Technology}, volume={7}, ISSN={2472-5552}, url={http://dx.doi.org/10.1177/108705710200700310}, DOI={10.1177/108705710200700310}, number={3}, journal={SLAS Discovery}, publisher={Elsevier BV}, author={Préaudat, M. and Ouled-Diaf, J. and Alpha-Bazin, B. and Mathis, G. and Mitsugi, T. and Aono, Y. and Takahashi, K. and Takemoto, H.}, year={2002}, month=jun, pages={267–274} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        4,
        2
      ]
    ],
    "date-time": "2025-04-02T14:02:06Z",
    "timestamp": 1743602526232
  },
  "reference-count": 18,
  "publisher": "Elsevier BV",
  "issue": "3",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2002,
            6,
            1
          ]
        ],
        "date-time": "2002-06-01T00:00:00Z",
        "timestamp": 1022889600000
      },
      "content-version": "tdm",
      "delay-in-days": 0,
      "URL": "https://www.elsevier.com/tdm/userlicense/1.0/"
    },
    {
      "start": {
        "date-parts": [
          [
            2022,
            4,
            27
          ]
        ],
        "date-time": "2022-04-27T00:00:00Z",
        "timestamp": 1651017600000
      },
      "content-version": "vor",
      "delay-in-days": 7270,
      "URL": "http://creativecommons.org/licenses/by-nc-nd/4.0/"
    }
  ],
  "content-domain": {
    "domain": [
      "slas-discovery.org",
      "elsevier.com",
      "sciencedirect.com"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        2002,
        6
      ]
    ]
  },
  "DOI": "10.1177/108705710200700310",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2005,
        5,
        17
      ]
    ],
    "date-time": "2005-05-17T16:43:06Z",
    "timestamp": 1116348186000
  },
  "page": "267-274",
  "update-policy": "http://dx.doi.org/10.1016/elsevier_cm_policy",
  "source": "Crossref",
  "is-referenced-by-count": 32,
  "title": "A Homogeneous Caspase-3 Activity Assay Using HTRF\u00ae Technology",
  "prefix": "10.1016",
  "volume": "7",
  "author": [
    {
      "given": "M.",
      "family": "Pr\u00e9audat",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "J.",
      "family": "Ouled-Diaf",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "B.",
      "family": "Alpha-Bazin",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "G.",
      "family": "Mathis",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "T.",
      "family": "Mitsugi",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Y.",
      "family": "Aono",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "K.",
      "family": "Takahashi",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "H.",
      "family": "Takemoto",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "78",
  "reference": [
    {
      "key": "10.1177/108705710200700310_bib1",
      "doi-asserted-by": "crossref",
      "first-page": "37",
      "DOI": "10.1038/376037a0",
      "article-title": "Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis",
      "volume": "376",
      "author": "Nicholson",
      "year": "1995",
      "journal-title": "Nature"
    },
    {
      "key": "10.1177/108705710200700310_bib2",
      "doi-asserted-by": "crossref",
      "first-page": "1957",
      "DOI": "10.1084/jem.183.5.1957",
      "article-title": "Apopain/CPP32 cleaves proteins that are essential for cellular repair: a fundamental principle of apoptotic death",
      "volume": "183",
      "author": "Casciola-Rosen",
      "year": "1996",
      "journal-title": "J Exp Med"
    },
    {
      "key": "10.1177/108705710200700310_bib3",
      "unstructured": "Karvinen J, Andersson K, Hurskainen P, et al: Sensitive homogeneous protease assay based on time resolved fluorometry. Poster presented at the 6th Annual Conference and Exhibition of the Society for Biomolecular Screening, September 2000, Vancouver, British Columbia, Canada."
    },
    {
      "key": "10.1177/108705710200700310_bib4",
      "doi-asserted-by": "crossref",
      "first-page": "30",
      "DOI": "10.1038/sj.cdd.4400769",
      "article-title": "Quantitative analysis of fluorescent caspase substrate cleavage in intact cells and identification of novel inhibitors of apoptosis",
      "volume": "8",
      "author": "Tawa",
      "year": "2001",
      "journal-title": "Cell Growth Differ"
    },
    {
      "key": "10.1177/108705710200700310_bib5",
      "doi-asserted-by": "crossref",
      "first-page": "203",
      "DOI": "10.1177/108705719600100407",
      "article-title": "Use of a novel homogeneous fluorescent technology in high throughput screening",
      "volume": "1",
      "author": "Kolb",
      "year": "1996",
      "journal-title": "J Biomol Screen"
    },
    {
      "key": "10.1177/108705710200700310_bib6",
      "doi-asserted-by": "crossref",
      "first-page": "91",
      "DOI": "10.1177/108705719800300205",
      "article-title": "Development of a CD28/CD86 (B7-2) binding assay for high throughput screening by homogeneous time-resolved fluorescence",
      "volume": "3",
      "author": "Mellor",
      "year": "1998",
      "journal-title": "J Biomol Screen"
    },
    {
      "key": "10.1177/108705710200700310_bib7",
      "doi-asserted-by": "crossref",
      "first-page": "266",
      "DOI": "10.1002/anie.198702661",
      "article-title": "Energy transfer luminescence of europium(III) and terbium(III) cryptates of macrobicyclic polypyridine ligands",
      "volume": "26",
      "author": "Alpha",
      "year": "1987",
      "journal-title": "Angew Chem"
    },
    {
      "key": "10.1177/108705710200700310_bib8",
      "doi-asserted-by": "crossref",
      "first-page": "1953",
      "DOI": "10.1093/clinchem/39.9.1953",
      "article-title": "Rare earth cryptates and homogeneous fluoroimmunoassays with human sera",
      "volume": "39",
      "author": "Mathis",
      "year": "1993",
      "journal-title": "Clin Chem"
    },
    {
      "key": "10.1177/108705710200700310_bib9",
      "doi-asserted-by": "crossref",
      "first-page": "2197",
      "DOI": "10.1016/S1386-1425(01)00493-0",
      "article-title": "Homogeneous time resolved fluorescence energy transfer using rare earth cryptates as a tool for probing molecular interactions in biology",
      "volume": "57",
      "author": "Bazin",
      "year": "2001",
      "journal-title": "Spectrochim Acta A Mol Biomol Spectrosc"
    },
    {
      "key": "10.1177/108705710200700310_bib10",
      "unstructured": "Mathis G, Preaudat M, Trinquet E, et al: A new homogeneous method using rare earth cryptate and amplification by energy transfer for the characterization of the FOS and Jun leucine zipper peptides dimerization. In Proceedings of H.T.S. for Drug Development meeting, 1994, Philadelphia, PA."
    },
    {
      "key": "10.1177/108705710200700310_bib11",
      "unstructured": "Park YW, Cummings R, Hermes JD: Proximity high throughput kinase assay. Presented at the 3rd Annual Conference and Exhibition of the Society for Biomolecular Screening, September 1997, San Diego, CA."
    },
    {
      "key": "10.1177/108705710200700310_bib12",
      "doi-asserted-by": "crossref",
      "first-page": "2338",
      "DOI": "10.1038/sj.onc.1203554",
      "article-title": "Sensitization to CD95 ligand-induced apoptosis in human glioma cells by hyperthermia involves enhanced cytochrome C release",
      "volume": "19",
      "author": "Hermisson",
      "year": "2000",
      "journal-title": "Oncogene"
    },
    {
      "key": "10.1177/108705710200700310_bib13",
      "doi-asserted-by": "crossref",
      "first-page": "1312",
      "DOI": "10.1126/science.281.5381.1312",
      "article-title": "Caspase: enemies within",
      "volume": "281",
      "author": "Thornberry",
      "year": "1998",
      "journal-title": "Science"
    },
    {
      "key": "10.1177/108705710200700310_bib14",
      "unstructured": "Mabile M, Mathis G, Jolu EJP, et al: Method for measuring the luminescence in a luminescent assay. Patent WO 9213264; US 5527684, 1991."
    },
    {
      "key": "10.1177/108705710200700310_bib15",
      "doi-asserted-by": "crossref",
      "first-page": "723",
      "DOI": "10.1038/380723a0",
      "article-title": "Sequential activation of ICE-like and CPP32-like protease during Fas-mediated apoptosis",
      "volume": "380",
      "author": "Enari",
      "year": "1996",
      "journal-title": "Nature"
    },
    {
      "key": "10.1177/108705710200700310_bib16",
      "doi-asserted-by": "crossref",
      "first-page": "920",
      "DOI": "10.1006/bbrc.1997.7370",
      "article-title": "Recombinant caspase-3 expressed in Pichia pastoris is fully activated and kinetically indistinguishable from the native enzyme",
      "volume": "238",
      "author": "Sun",
      "year": "1997",
      "journal-title": "Biochem Biophys Res Common"
    },
    {
      "key": "10.1177/108705710200700310_bib17",
      "doi-asserted-by": "crossref",
      "first-page": "263",
      "DOI": "10.1177/108705710000500409",
      "article-title": "High throughput screening for human interferon-y production inhibitor using homogeneous time resolved fluorescence",
      "volume": "5",
      "author": "Enomoto",
      "year": "2000",
      "journal-title": "J Biomol Screen"
    },
    {
      "key": "10.1177/108705710200700310_bib18",
      "doi-asserted-by": "crossref",
      "first-page": "279",
      "DOI": "10.2174/1386207302666220204200224",
      "article-title": "Application of homogeneous time resolved fluorescence (HTRF\u00ae) to monitor polyubiquitination of wild type p-53",
      "volume": "2",
      "author": "Yabuki",
      "year": "1999",
      "journal-title": "Comb Chem High Throughput Screen"
    }
  ],
  "container-title": "SLAS Discovery",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "http://journals.sagepub.com/doi/pdf/10.1177/108705710200700310",
      "content-type": "application/pdf",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://api.elsevier.com/content/article/PII:S2472630322001157?httpAccept=text/xml",
      "content-type": "text/xml",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://api.elsevier.com/content/article/PII:S2472630322001157?httpAccept=text/plain",
      "content-type": "text/plain",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "http://journals.sagepub.com/doi/pdf/10.1177/108705710200700310",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2022,
        11,
        19
      ]
    ],
    "date-time": "2022-11-19T18:39:11Z",
    "timestamp": 1668883151000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://linkinghub.elsevier.com/retrieve/pii/S2472630322001157"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        2002,
        6
      ]
    ]
  },
  "references-count": 18,
  "journal-issue": {
    "issue": "3",
    "published-print": {
      "date-parts": [
        [
          2002,
          6
        ]
      ]
    }
  },
  "alternative-id": [
    "S2472630322001157"
  ],
  "URL": "http://dx.doi.org/10.1177/108705710200700310",
  "relation": {},
  "ISSN": [
    "2472-5552"
  ],
  "subject": [],
  "container-title-short": "SLAS Discovery",
  "published": {
    "date-parts": [
      [
        2002,
        6
      ]
    ]
  },
  "assertion": [
    {
      "value": "Elsevier",
      "name": "publisher",
      "label": "This article is maintained by"
    },
    {
      "value": "A Homogeneous Caspase-3 Activity Assay Using HTRF\u00ae Technology",
      "name": "articletitle",
      "label": "Article Title"
    },
    {
      "value": "SLAS Discovery",
      "name": "journaltitle",
      "label": "Journal Title"
    },
    {
      "value": "https://doi.org/10.1177/108705710200700310",
      "name": "articlelink",
      "label": "CrossRef DOI link to publisher maintained version"
    },
    {
      "value": "article",
      "name": "content_type",
      "label": "Content Type"
    },
    {
      "value": "Copyright \u00a9 2002 Society for Laboratory Automation and Screening. Published by Elsevier Inc.",
      "name": "copyright",
      "label": "Copyright"
    }
  ]
}