DOI: 10.1177/030089168907500505. Sensitivity Pattern of Normal and Ha-Ras Transformed Nih3T3 Fibroblasts to Antineoplastic Drugs

Sensitivity Pattern of Normal and Ha-Ras Transformed Nih3T3 Fibroblasts to Antineoplastic Drugs

10.1177/030089168907500505

Crossref journal-article

SAGE Publications

Tumori Journal (179)

Abstract

Ha-ras-transformed NIH3T3 fibroblasts were compared with the parental cell line to investigate the influence of the Ha-ras oncogene on cellular chemosensitivity to antineoplastic drugs. Four NIH3T3 cell clones independently transformed by the Ha-ras oncogene, activated by mutation or overexpression, were analyzed: 3 clones were obtained by transfection of NIH3T3 cells with a mutation-activated Ha-ras gene and 1 clone by transfection of a large copy number of the normal Ha-ras protooncogene. Chemosensitivity of the transformed clones and of the parental cell line was analyzed when cells were in the same condition of proliferative activity and cell cycle phase distribution. No significant differences in chemosensitivity were observed between transformed and untrans-formed cell lines to doxorubicin, VP-16, cis-platinum or mitomycin C. Therefore, data suggest that activated Ha-ras oncogenes have no role in sensitivity to these antineoplastic agents.

Bibliography

Toffoli, G., Viel, A., Tumiotto, L., Buttazzi, P., Biscontin, G., & Boiocchi, M. (1989). Sensitivity Pattern of Normal and Ha-Ras Transformed Nih3T3 Fibroblasts to Antineoplastic Drugs. Tumori Journal, 75(5), 423â428.

Authors 6

Giuseppe Toffoli (first)
Alessandra Viel (additional)
Loretta Tumiotto (additional)
Patrizio Buttazzi (additional)
Gabriella Biscontin (additional)
Mauro Boiocchi (additional)

References 22 Referenced 13

10.1016/S0021-9258(17)34875-5 / J. Biol. Chem. by Alt F. (1978)
10.1002/j.1460-2075.1985.tb03974.x
10.1038/297479a0
{'key': 'bibr4-030089168907500505', 'first-page': '5290', 'volume': '47', 'author': 'Clair T.', 'year': '1987', 'journal-title': 'Cancer Res.'} / Cancer Res. by Clair T. (1987)
10.1016/0076-6879(67)12123-X
{'key': 'bibr6-030089168907500505', 'first-page': '87', 'volume': '68', 'author': 'Curt G.A.', 'year': '1984', 'journal-title': 'Cancer Treat. Rep.'} / Cancer Treat. Rep. by Curt G.A. (1984)
{'key': 'bibr7-030089168907500505', 'first-page': '25', 'volume': '1', 'author': 'Gerlach J.H.', 'year': '1986', 'journal-title': 'Cancer Surveys'} / Cancer Surveys by Gerlach J.H. (1986)
{'key': 'bibr8-030089168907500505', 'first-page': '3643', 'volume': '44', 'author': 'Goldie J.H.', 'year': '1984', 'journal-title': 'Cancer Res.'} / Cancer Res. by Goldie J.H. (1984)
{'key': 'bibr9-030089168907500505', 'first-page': '143', 'volume': '36', 'author': 'Krishan A.', 'year': '1976', 'journal-title': 'Cancer Res.'} / Cancer Res. by Krishan A. (1976)
{'key': 'bibr10-030089168907500505', 'first-page': '209', 'volume-title': 'In: Molecular Cloning: a Laboratory Manual.', 'author': 'Maniatis T.', 'year': '1982'} / In: Molecular Cloning: a Laboratory Manual. by Maniatis T. (1982)
10.1038/310644a0
10.1128/MCB.3.10.1783
10.1002/jcb.240200106
10.1073/pnas.79.9.2845
10.1128/MCB.5.10.2836
10.1038/309626a0
{'key': 'bibr17-030089168907500505', 'first-page': '793', 'volume': '48', 'author': 'Sklar M.D.', 'year': '1988', 'journal-title': 'Cancer Res.'} / Cancer Res. by Sklar M.D. (1988)
10.1126/science.6538699
10.1016/S0022-2836(75)80083-0
10.1146/annurev.ge.18.120184.003005
10.1126/science.2997917
10.1016/0092-8674(79)90093-X

Dates

Type	When
Created	7 years, 4 months ago (April 17, 2018, 5:35 a.m.)
Deposited	6 months, 1 week ago (Feb. 28, 2025, 1:42 p.m.)
Indexed	3 months, 1 week ago (May 29, 2025, 5:35 a.m.)
Issued	35 years, 11 months ago (Oct. 1, 1989)
Published	35 years, 11 months ago (Oct. 1, 1989)
Published Online	35 years, 11 months ago (Oct. 1, 1989)
Published Print	35 years, 11 months ago (Oct. 1, 1989)

Funders 0

None

BibTeX

@article{Toffoli_1989, title={Sensitivity Pattern of Normal and Ha-Ras Transformed Nih3T3 Fibroblasts to Antineoplastic Drugs}, volume={75}, ISSN={2038-2529}, url={http://dx.doi.org/10.1177/030089168907500505}, DOI={10.1177/030089168907500505}, number={5}, journal={Tumori Journal}, publisher={SAGE Publications}, author={Toffoli, Giuseppe and Viel, Alessandra and Tumiotto, Loretta and Buttazzi, Patrizio and Biscontin, Gabriella and Boiocchi, Mauro}, year={1989}, month=oct, pages={423–428} }

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