DOI: 10.1177/00220345970760070501. Expression of Connexin 43 in Rat Mandibular Bone and Periodontal Ligament (PDL) Cells during Experimental Tooth Movement

Expression of Connexin 43 in Rat Mandibular Bone and Periodontal Ligament (PDL) Cells during Experimental Tooth Movement

10.1177/00220345970760070501

Crossref journal-article

SAGE Publications

Journal of Dental Research (179)

Abstract

Bone remodeling in response to force requires the coordinated action of osteoblasts, osteoclasts, osteocytes, and periodontal ligament cells. Coordination among these cells may be mediated, in part, by cell-to-cell communication via gap junctions. This study tests the hypothesis that the regulation of expression of connexin 43, a gap junction protein, is part of the transduction mechanism between force as applied to bone during orthodontic tooth movement and bone remodeling. To test this hypothesis, we examined connexin 43 expression in a rat model system of experimental tooth movement. To establish the model, we extracted maxillary first molars to initiate supra-eruption of opposing mandibular molars. The rats were killed at 0, 6, 12, 24, and 48 hrs post-extraction. The mandibles were removed, demineralized, and embedded in paraffin. To localize connexin 43 protein and mRNA, we used a specific antibody for immunohistochemistry and a specific cDNA probe for in situ hybridization. Western and Northern blot analyses were used to assess the specificity of the connexin 43 antibody and cDNA probe, respectively. We found connexin 43 protein expressed by osteoclasts (++++) and periodontal ligament cells (+++) in compression zones, and by osteoblasts (++++) and osteocytes (++++) in tension zones of the periodontal ligament. In addition, connexin 43 mRNA was found in some bone and periodontal ligament cells. Connexin 43 protein was found, by densitometric analysis, to be higher in the periodontal ligament after exposure to force compared with controls (P < 0.001). The number of osteocytes expressing connexin 43 48 hrs after molar extraction was also significantly greater in bone subjected to tension when compared with controls (P < 0.001). The results of this study support the hypothesis that connexin 43 plays a role in the coordination of events during experimentally induced alveolar bone remodeling.

Bibliography

Su, M., Borke, J. L., Donahue, H. J., Li, Z., Warshawsky, N. M., Russell, C. M., & Lewis, J. E. (1997). Expression of Connexin 43 in Rat Mandibular Bone and Periodontal Ligament (PDL) Cells during Experimental Tooth Movement. Journal of Dental Research, 76(7), 1357â1366.

Authors 7

M. Su (first)
J.L. Borke (additional)
H.J. Donahue (additional)
Z. Li (additional)
N.M. Warshawsky (additional)
C.M. Russell (additional)
J.E. Lewis (additional)

References 26 Referenced 66

10.1083/jcb.105.6.2621
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10.1172/JCI113196
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10.1007/BF00185892
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10.1161/01.RES.66.4.1074
{'key': 'atypb10', 'first-page': 'G911', 'volume': '265', 'author': 'Li Z.', 'year': '1993', 'journal-title': 'Am J Physiol'} / Am J Physiol by Li Z. (1993)
Makowski L. (1988). X-ray diffraction studies of gap junction structure . In: Advances in cell biology. Vol 2. Miller K, editor. Greenwich, CT: Jai Press, pp. 119-158. (10.1016/S1569-2558(08)60432-4)
10.1146/annurev.me.38.020187.001021
10.1083/jcb.111.5.2077
10.1016/0003-9969(94)90119-8
10.1016/8756-3282(90)90134-K
Reitan K. (1975). Biomechanical principles and reactions. In: Current orthodontic concepts and techniques. Graber TM, Swain BF, editors. Philadelphia: Saunders , pp. 111-229.
10.1177/00220345810600021501
Rodan GA, Raisz LG (1990). Cellular basis for bone turnover. In: Metabolic bone diseases and clinically related disorders. Avioli LV, Krane SM, editors. Philadelphia: Saunders , pp. 1-41.
10.1016/0003-9969(93)90104-T
10.1016/0889-5406(91)70005-H
{'key': 'atypb21', 'first-page': '1433', 'volume': '6', 'author': 'Schiller PC', 'year': '1992', 'journal-title': 'Mol Endocrinol'} / Mol Endocrinol by Schiller PC (1992)
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10.1073/pnas.76.9.4350
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10.1177/00220345800590101301

Dates

Type	When
Created	16 years, 7 months ago (Jan. 8, 2009, 7:39 p.m.)
Deposited	5 months, 4 weeks ago (March 3, 2025, 1:54 a.m.)
Indexed	3 months, 1 week ago (May 19, 2025, 8:08 a.m.)
Issued	28 years, 2 months ago (July 1, 1997)
Published	28 years, 2 months ago (July 1, 1997)
Published Online	28 years, 2 months ago (July 1, 1997)
Published Print	28 years, 2 months ago (July 1, 1997)

Funders 0

None

BibTeX

@article{Su_1997, title={Expression of Connexin 43 in Rat Mandibular Bone and Periodontal Ligament (PDL) Cells during Experimental Tooth Movement}, volume={76}, ISSN={1544-0591}, url={http://dx.doi.org/10.1177/00220345970760070501}, DOI={10.1177/00220345970760070501}, number={7}, journal={Journal of Dental Research}, publisher={SAGE Publications}, author={Su, M. and Borke, J.L. and Donahue, H.J. and Li, Z. and Warshawsky, N.M. and Russell, C.M. and Lewis, J.E.}, year={1997}, month=jul, pages={1357–1366} }

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