DOI: 10.1177/00220345890680060301. Bovine Tooth-derived Bone Morphogenetic Protein

Bovine Tooth-derived Bone Morphogenetic Protein

10.1177/00220345890680060301

Crossref journal-article

SAGE Publications

Journal of Dental Research (179)

Abstract

Eight groups of dental tissues were mechanically dissected from the mandibles of one-year-old steers; they were then defatted and decalcified in HCI. The noncollagenous proteins were extracted with various solvents from collections of tissue and bio-assayed for osteo-inductive activity. Collectively, the hard tissue (dentin, enamel, and cementum) noncollagenous proteins were fractionated by molecular sieve chromatography, hydroxyapatite affinity chromatography, and ion exchange chromatography. Osteo-inductive activity of each protein fraction was determined by implantation in the quadriceps muscle pouch of mice. The quantity of bone was measured by computerized image analysis. From 71% to 83% of 41 implants of dental hard tissues induced bone formation. The quantity of bone was greater from unerupted than from erupted teeth. Dental soft tissues that had no osteo-inductive activity were rich in a 14-kDa protein, presumably matrix gamma-carboxyglutamic acid-rich proteins. Proteins with Mr of from 15 to 28 kDa were associated with osteo-inductive activity. Components with Mr greater than 28 kDa had no activity. These observations suggest that bovine teeth have a selection of osteo-inductive proteins that is comparable in range of MW to bovine bone morphogenetic protein.

Bibliography

Kawai, T., & Urist, M. R. (1989). Bovine Tooth-derived Bone Morphogenetic Protein. Journal of Dental Research, 68(6), 1069â1074.

Authors 2

T. Kawai (first)
M.R. Urist (additional)

References 21 Referenced 66

{'key': 'atypb1', 'first-page': '294', 'volume': '151', 'author': 'Ashton, B.A.', 'year': '1980', 'journal-title': 'Clin Orthop'} / Clin Orthop by Ashton, B.A. (1980)
10.1001/archsurg.1967.01330120035008
10.1177/00220345790580091201
{'volume-title': 'Proceedings of the 1st International Conference on Chemistry and Biology of Mineralized Connective Tissue, Northwestern University', 'author': 'Conover, M.A.', 'key': 'atypb4'} / Proceedings of the 1st International Conference on Chemistry and Biology of Mineralized Connective Tissue, Northwestern University by Conover, M.A.
10.1097/00003086-198005000-00045 / Clin Orthop by Hanamura, H. (1980)
10.1084/jem.132.6.1250
{'key': 'atypb7', 'first-page': '12', 'volume': '65', 'author': 'Inoue, T.', 'year': '1986', 'journal-title': ', and Periodontal Ligament by Demineralized Dentin and Bone Matrix in vivo and in vitro, J Dent Res'} / , and Periodontal Ligament by Demineralized Dentin and Bone Matrix in vivo and in vitro, J Dent Res by Inoue, T. (1986)
10.1097/00003086-198808000-00032 / Clin Orthop by Kawai, T. (1988)
10.1097/00003086-198007000-00049 / Clin Orthop by Lindholm, T.S. (1980)
10.1097/00003086-198211000-00039 / Clin Orthop by Mizutani, H. (1982)
10.1016/0006-291X(83)91663-7
10.14219/jada.archive.1940.0340
10.1083/jcb.97.6.1950
10.1073/pnas.82.8.2267
10.1097/00003086-198005000-00044 / Clin Orthop by Takaoka, K. (1980)
10.1126/science.150.3698.893
{'key': 'atypb17', 'first-page': '88', 'volume': '29', 'author': 'Urist, M.R.', 'year': '1971', 'journal-title': 'Oral Surg Oral Med Oral Pathol'} / Oral Surg Oral Med Oral Pathol by Urist, M.R. (1971)
10.1073/pnas.81.2.371
10.1073/pnas.70.12.3511
Urist, M.R.; Mizutani, H.; Conover, M.A.; Lietz, A.; and Finerman, G.A.M. (1982): Bone, Dentin, and Osteosarcoma Bone Morphogenetic Proteins. In: Factors and Mechanisms Influencing Bone Growth, A.D. Dixon and B. Sarnat, Eds. New York: Alan R. Liss, pp. 61-81.
10.1016/0003-9969(67)90095-7

Dates

Type	When
Created	14 years, 8 months ago (Nov. 28, 2010, 9:14 p.m.)
Deposited	5 months, 3 weeks ago (March 3, 2025, 3:54 p.m.)
Indexed	1 month, 1 week ago (July 16, 2025, 9:34 a.m.)
Issued	36 years, 2 months ago (June 1, 1989)
Published	36 years, 2 months ago (June 1, 1989)
Published Online	36 years, 2 months ago (June 1, 1989)
Published Print	36 years, 2 months ago (June 1, 1989)

Funders 0

None

BibTeX

@article{Kawai_1989, title={Bovine Tooth-derived Bone Morphogenetic Protein}, volume={68}, ISSN={1544-0591}, url={http://dx.doi.org/10.1177/00220345890680060301}, DOI={10.1177/00220345890680060301}, number={6}, journal={Journal of Dental Research}, publisher={SAGE Publications}, author={Kawai, T. and Urist, M.R.}, year={1989}, month=jun, pages={1069–1074} }

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