DOI: 10.1177/000456328902600213. Cytosolic Protein-Tyrosine Kinase Activities in Various Rat Tissues

Cytosolic Protein-Tyrosine Kinase Activities in Various Rat Tissues

10.1177/000456328902600213

Crossref journal-article

SAGE Publications

Annals of Clinical Biochemistry: International Journal of Laboratory Medicine (179)

Abstract

Suitable assay conditions for the detection of cytosolic protein-tyrosine kinase activities in crude extracts of various rat tissues have been determined. Cytosolic protein-tyrosine kinases showed common characteristics including substrate specificity and divalent cation requirement. Using (Val5) angiotensin II and Mn2+ rather than a src-related synthetic peptide, E11G1, and Mg2+, we obtained higher activities of cytosolic protein-tyrosine kinases. Among various rat tissues tested, spleen, bone marrow, thymus, small intestine, appendix and lung, in decreasing order of total activity, contained high activities of cytosolic protein-tyrosine kinases. These results suggest that the enzyme activities in lymphatic organs and in organs closely related to cell proliferation are high. The assay system described allows the precise measurement of cytosolic protein-tyrosine kinase activity in various rat tissues, both normal and malignant.

Bibliography

Kobayashi, T., Nakamura, S.-I., & Yamamura, H. (1989). Cytosolic Protein-Tyrosine Kinase Activities in Various Rat Tissues. Annals of Clinical Biochemistry: International Journal of Laboratory Medicine, 26(2), 164â168.

Authors 3

Tomoko Kobayashi (first)
Shun-Ichi Nakamura (additional)
Hirohei Yamamura (additional)

References 22 Referenced 15

10.1146/annurev.bi.52.070183.001505
10.1016/S0021-9258(18)43497-7 / J Biol Chem. by Ushiro H (1980)
10.1038/295419a0
10.1016/S0021-9258(18)33955-3 / J Biol Chem. by Kasuga M (1982)
10.1038/305438a0
10.1016/S0092-8674(85)80047-7
10.1073/pnas.82.21.7275
10.1073/pnas.84.8.2251
10.1016/S0006-291X(84)80338-1
{'key': 'bibr10-000456328902600213', 'first-page': '13754', 'volume': '261', 'author': 'Rosen N', 'year': '1986', 'journal-title': 'J Cell BioChem.'} / J Cell BioChem. by Rosen N (1986)
10.1146/annurev.bi.54.070185.004341
10.1073/pnas.80.9.2529
10.1016/0014-5793(84)81386-1
10.1016/S0021-9258(17)39759-4 / J Biol Chem. by Wong TW (1984)
10.1016/0014-5793(85)80652-9
10.1111/j.1432-1033.1988.tb14122.x
10.1016/S0021-9258(18)66763-8 / J Biol Chem. by Zioncheck TF (1986)
10.1016/S0006-291X(88)80401-7
10.1016/S0021-9258(18)89633-8 / J Biol Chem. by Lin MF (1985)
10.1159/000217522
10.1016/S0021-9258(17)44462-0 / J Biol Chem. by Swarup G (1983)
10.1016/0003-2697(76)90527-3

Dates

Type	When
Created	11 years, 6 months ago (Feb. 5, 2014, 4:57 p.m.)
Deposited	5 months, 3 weeks ago (March 4, 2025, 12:41 p.m.)
Indexed	5 months, 3 weeks ago (March 5, 2025, 12:43 a.m.)
Issued	36 years, 5 months ago (March 1, 1989)
Published	36 years, 5 months ago (March 1, 1989)
Published Online	36 years, 5 months ago (March 1, 1989)
Published Print	36 years, 5 months ago (March 1, 1989)

Funders 0

None

BibTeX

@article{Kobayashi_1989, title={Cytosolic Protein-Tyrosine Kinase Activities in Various Rat Tissues}, volume={26}, ISSN={1758-1001}, url={http://dx.doi.org/10.1177/000456328902600213}, DOI={10.1177/000456328902600213}, number={2}, journal={Annals of Clinical Biochemistry: International Journal of Laboratory Medicine}, publisher={SAGE Publications}, author={Kobayashi, Tomoko and Nakamura, Shun-Ichi and Yamamura, Hirohei}, year={1989}, month=mar, pages={164–168} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        3,
        5
      ]
    ],
    "date-time": "2025-03-05T05:43:26Z",
    "timestamp": 1741153406733,
    "version": "3.38.0"
  },
  "reference-count": 22,
  "publisher": "SAGE Publications",
  "issue": "2",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            1989,
            3,
            1
          ]
        ],
        "date-time": "1989-03-01T00:00:00Z",
        "timestamp": 604713600000
      },
      "content-version": "tdm",
      "delay-in-days": 0,
      "URL": "https://journals.sagepub.com/page/policies/text-and-data-mining-license"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        1989,
        3
      ]
    ]
  },
  "abstract": "<jats:p> Suitable assay conditions for the detection of cytosolic protein-tyrosine kinase activities in crude extracts of various rat tissues have been determined. Cytosolic protein-tyrosine kinases showed common characteristics including substrate specificity and divalent cation requirement. Using (Val<jats:sup>5</jats:sup>) angiotensin II and Mn<jats:sup>2+</jats:sup> rather than a src-related synthetic peptide, E<jats:sub>11</jats:sub>G<jats:sub>1</jats:sub>, and Mg<jats:sup>2+</jats:sup>, we obtained higher activities of cytosolic protein-tyrosine kinases. Among various rat tissues tested, spleen, bone marrow, thymus, small intestine, appendix and lung, in decreasing order of total activity, contained high activities of cytosolic protein-tyrosine kinases. These results suggest that the enzyme activities in lymphatic organs and in organs closely related to cell proliferation are high. The assay system described allows the precise measurement of cytosolic protein-tyrosine kinase activity in various rat tissues, both normal and malignant. </jats:p>",
  "DOI": "10.1177/000456328902600213",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2014,
        2,
        5
      ]
    ],
    "date-time": "2014-02-05T21:57:08Z",
    "timestamp": 1391637428000
  },
  "page": "164-168",
  "source": "Crossref",
  "is-referenced-by-count": 15,
  "title": "Cytosolic Protein-Tyrosine Kinase Activities in Various Rat Tissues",
  "prefix": "10.1177",
  "volume": "26",
  "author": [
    {
      "given": "Tomoko",
      "family": "Kobayashi",
      "sequence": "first",
      "affiliation": [
        {
          "name": "Department of Biochemistry, Fukui Medical School, Fukui 910-11, Japan"
        }
      ]
    },
    {
      "given": "Shun-Ichi",
      "family": "Nakamura",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Biochemistry, Fukui Medical School, Fukui 910-11, Japan"
        }
      ]
    },
    {
      "given": "Hirohei",
      "family": "Yamamura",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Biochemistry, Fukui Medical School, Fukui 910-11, Japan"
        }
      ]
    }
  ],
  "member": "179",
  "published-online": {
    "date-parts": [
      [
        1989,
        3,
        1
      ]
    ]
  },
  "reference": [
    {
      "key": "bibr1-000456328902600213",
      "doi-asserted-by": "publisher",
      "DOI": "10.1146/annurev.bi.52.070183.001505"
    },
    {
      "key": "bibr2-000456328902600213",
      "doi-asserted-by": "crossref",
      "first-page": "8363",
      "DOI": "10.1016/S0021-9258(18)43497-7",
      "volume": "255",
      "author": "Ushiro H",
      "year": "1980",
      "journal-title": "J Biol Chem."
    },
    {
      "key": "bibr3-000456328902600213",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/295419a0"
    },
    {
      "key": "bibr4-000456328902600213",
      "doi-asserted-by": "crossref",
      "first-page": "9891",
      "DOI": "10.1016/S0021-9258(18)33955-3",
      "volume": "257",
      "author": "Kasuga M",
      "year": "1982",
      "journal-title": "J Biol Chem."
    },
    {
      "key": "bibr5-000456328902600213",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/305438a0"
    },
    {
      "key": "bibr6-000456328902600213",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(85)80047-7"
    },
    {
      "key": "bibr7-000456328902600213",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.82.21.7275"
    },
    {
      "key": "bibr8-000456328902600213",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.84.8.2251"
    },
    {
      "key": "bibr9-000456328902600213",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0006-291X(84)80338-1"
    },
    {
      "key": "bibr10-000456328902600213",
      "first-page": "13754",
      "volume": "261",
      "author": "Rosen N",
      "year": "1986",
      "journal-title": "J Cell BioChem."
    },
    {
      "key": "bibr11-000456328902600213",
      "doi-asserted-by": "publisher",
      "DOI": "10.1146/annurev.bi.54.070185.004341"
    },
    {
      "key": "bibr12-000456328902600213",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.80.9.2529"
    },
    {
      "key": "bibr13-000456328902600213",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0014-5793(84)81386-1"
    },
    {
      "key": "bibr14-000456328902600213",
      "doi-asserted-by": "crossref",
      "first-page": "8505",
      "DOI": "10.1016/S0021-9258(17)39759-4",
      "volume": "259",
      "author": "Wong TW",
      "year": "1984",
      "journal-title": "J Biol Chem."
    },
    {
      "key": "bibr15-000456328902600213",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0014-5793(85)80652-9"
    },
    {
      "key": "bibr16-000456328902600213",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1432-1033.1988.tb14122.x"
    },
    {
      "key": "bibr17-000456328902600213",
      "doi-asserted-by": "crossref",
      "first-page": "15637",
      "DOI": "10.1016/S0021-9258(18)66763-8",
      "volume": "261",
      "author": "Zioncheck TF",
      "year": "1986",
      "journal-title": "J Biol Chem."
    },
    {
      "key": "bibr18-000456328902600213",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0006-291X(88)80401-7"
    },
    {
      "key": "bibr19-000456328902600213",
      "doi-asserted-by": "crossref",
      "first-page": "1582",
      "DOI": "10.1016/S0021-9258(18)89633-8",
      "volume": "260",
      "author": "Lin MF",
      "year": "1985",
      "journal-title": "J Biol Chem."
    },
    {
      "key": "bibr20-000456328902600213",
      "doi-asserted-by": "publisher",
      "DOI": "10.1159/000217522"
    },
    {
      "key": "bibr21-000456328902600213",
      "doi-asserted-by": "crossref",
      "first-page": "10341",
      "DOI": "10.1016/S0021-9258(17)44462-0",
      "volume": "258",
      "author": "Swarup G",
      "year": "1983",
      "journal-title": "J Biol Chem."
    },
    {
      "key": "bibr22-000456328902600213",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0003-2697(76)90527-3"
    }
  ],
  "container-title": "Annals of Clinical Biochemistry: International Journal of Laboratory Medicine",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://journals.sagepub.com/doi/pdf/10.1177/000456328902600213",
      "content-type": "application/pdf",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://journals.sagepub.com/doi/pdf/10.1177/000456328902600213",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2025,
        3,
        4
      ]
    ],
    "date-time": "2025-03-04T17:41:58Z",
    "timestamp": 1741110118000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://journals.sagepub.com/doi/10.1177/000456328902600213"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1989,
        3
      ]
    ]
  },
  "references-count": 22,
  "journal-issue": {
    "issue": "2",
    "published-print": {
      "date-parts": [
        [
          1989,
          3
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1177/000456328902600213"
  ],
  "URL": "http://dx.doi.org/10.1177/000456328902600213",
  "relation": {},
  "ISSN": [
    "0004-5632",
    "1758-1001"
  ],
  "subject": [],
  "container-title-short": "Ann Clin Biochem",
  "published": {
    "date-parts": [
      [
        1989,
        3
      ]
    ]
  }
}