DOI: 10.1161/01.res.73.3.492. ATP-dependent closure and reactivation of inward rectifier K+ channels in endothelial cells.

ATP-dependent closure and reactivation of inward rectifier K+ channels in endothelial cells.

10.1161/01.res.73.3.492

Crossref journal-article

Ovid Technologies (Wolters Kluwer Health)

Circulation Research (276)

Abstract

This report presents the results of a patch-clamp study examining the role of intracellular ATP in the regulation of endothelial inward rectifier K+ channels. Administration of ATP to the cytosolic surface of inside-out patches reversibly activated the K+ channel within seconds. ATP (1 mM) increased the mean open probability by a factor of 3.5, primarily by increasing the number of openings. Administration of the nonhydrolyzable ATP analogue ATP-gamma-S failed to modulate channel activity. Inhibition of ATP synthesis by administration of cyanide plus iodoacetate resulted in channel closure within 1 to 6 minutes. In experiments in which ATP was coadministered with the metabolic blockers, the channel activity continued unchanged for up to 30 minutes, but when ATP was removed, the activity rapidly decayed. We propose that normal functioning of the inward rectifier K+ channel is ATP dependent. Phosphorylation of the channel molecule is probably essential for maintaining activity.

Bibliography

Olesen, S. P., & Bundgaard, M. (1993). ATP-dependent closure and reactivation of inward rectifier K+ channels in endothelial cells. Circulation Research, 73(3), 492â495.

Authors 2

S P Olesen (first)
M Bundgaard (additional)

References 0 Referenced 26

None

Dates

Type	When
Created	13 years, 2 months ago (June 11, 2012, 8:47 p.m.)
Deposited	1 year, 3 months ago (May 12, 2024, 4:24 p.m.)
Indexed	1 year, 2 months ago (June 17, 2024, 4:37 p.m.)
Issued	31 years, 11 months ago (Sept. 1, 1993)
Published	31 years, 11 months ago (Sept. 1, 1993)
Published Print	31 years, 11 months ago (Sept. 1, 1993)

Funders 0

None

BibTeX

@article{Olesen_1993, title={ATP-dependent closure and reactivation of inward rectifier K+ channels in endothelial cells.}, volume={73}, ISSN={1524-4571}, url={http://dx.doi.org/10.1161/01.res.73.3.492}, DOI={10.1161/01.res.73.3.492}, number={3}, journal={Circulation Research}, publisher={Ovid Technologies (Wolters Kluwer Health)}, author={Olesen, S P and Bundgaard, M}, year={1993}, month=sep, pages={492–495} }

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