DOI: 10.1161/01.res.0000055016.36679.23. Focal Adhesion Kinase Is Required for Blood Vessel Morphogenesis

Focal Adhesion Kinase Is Required for Blood Vessel Morphogenesis

10.1161/01.res.0000055016.36679.23

Crossref journal-article

Ovid Technologies (Wolters Kluwer Health)

Circulation Research (276)

Abstract

The nonreceptor tyrosine kinase focal adhesion kinase (FAK) is a point of convergence for signals from extracellular matrix, soluble factors, and mechanical stimuli. Targeted disruption of the fak gene in mice leads to death at embryonic day 8.5 (E8.5). FAK −/− embryos have severely impaired blood vessel development. Gene expression and in vitro differentiation studies revealed that endothelial cell differentiation was comparable in FAK −/− and wild-type E8.5 embryos. We examined the role of FAK in blood vessel morphogenesis using an in vitro tubulogenesis assay and three different culture systems: FAK +/+ and FAK −/− embryoid bodies, FAK +/+ and FAK −/− endothelial cells, and human umbilical vein endothelial cells expressing antisense FAK, a dominant-negative fragment of FAK, or wild-type FAK. In all of these systems, endothelial cells deficient in FAK expression or function displayed a severely reduced ability to form tubules in Matrigel. These studies demonstrate clearly that the vascular defects in FAK −/− mice result from the inability of FAK-deficient endothelial cells to organize themselves into vascular networks, rather than from defects in tissue-specific differentiation.

Bibliography

Ilic, D., Kovacic, B., McDonagh, S., Jin, F., Baumbusch, C., Gardner, D. G., & Damsky, C. H. (2003). Focal Adhesion Kinase Is Required for Blood Vessel Morphogenesis. Circulation Research, 92(3), 300â307.

Authors 7

Dusko Ilic (first)
Branka Kovacic (additional)
Susan McDonagh (additional)
Fang Jin (additional)
Clark Baumbusch (additional)
David G. Gardner (additional)
Caroline H. Damsky (additional)

References 39 Referenced 93

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Dates

Type	When
Created	22 years, 6 months ago (Feb. 20, 2003, 6:38 p.m.)
Deposited	1 year, 3 months ago (May 12, 2024, 2:36 p.m.)
Indexed	2 months, 3 weeks ago (June 3, 2025, 11:14 a.m.)
Issued	22 years, 6 months ago (Feb. 21, 2003)
Published	22 years, 6 months ago (Feb. 21, 2003)
Published Print	22 years, 6 months ago (Feb. 21, 2003)

Funders 0

None

BibTeX

@article{Ilic_2003, title={Focal Adhesion Kinase Is Required for Blood Vessel Morphogenesis}, volume={92}, ISSN={1524-4571}, url={http://dx.doi.org/10.1161/01.res.0000055016.36679.23}, DOI={10.1161/01.res.0000055016.36679.23}, number={3}, journal={Circulation Research}, publisher={Ovid Technologies (Wolters Kluwer Health)}, author={Ilic, Dusko and Kovacic, Branka and McDonagh, Susan and Jin, Fang and Baumbusch, Clark and Gardner, David G. and Damsky, Caroline H.}, year={2003}, month=feb, pages={300–307} }

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