DOI: 10.1161/01.cir.98.1.13. Genes for Apolipoprotein B and Microsomal Triglyceride Transfer Protein Are Expressed in the Heart

Genes for Apolipoprotein B and Microsomal Triglyceride Transfer Protein Are Expressed in the Heart

10.1161/01.cir.98.1.13

Crossref journal-article

Ovid Technologies (Wolters Kluwer Health)

Circulation (276)

Abstract

Background —Expression of both the apolipoprotein B (apoB) gene and the microsomal triglyceride transfer protein (MTP) gene is required for the assembly and secretion of triglyceride-rich lipoproteins in the liver and intestine. Both genes have been assumed to be silent in the heart. Methods and Results —Northern blot and RNase protection analyses showed that the apoB and MTP genes were expressed in the hearts of mice and humans. In situ hybridization studies revealed that the apoB mRNA was produced in cardiac myocytes. Electron microscopy of human cardiac myocytes revealed lipid-staining particles of relatively small diameter (≈250 Å) within the Golgi apparatus. Conclusions —These studies strongly suggest that the heart synthesizes and secretes apoB-containing lipoproteins.

Bibliography

Nielsen, L. B., VeÌniant, M., BoreÌn, J., Raabe, M., Wong, J. S., Tam, C., Flynn, L., Vanni-Reyes, T., Gunn, M. D., Goldberg, I. J., Hamilton, R. L., & Young, S. G. (1998). Genes for Apolipoprotein B and Microsomal Triglyceride Transfer Protein Are Expressed in the Heart. Circulation, 98(1), 13â16.

Authors 12

Lars B. Nielsen (first)
Murielle Véniant (additional)
Jan Borén (additional)
Martin Raabe (additional)
Jinny S. Wong (additional)
Carmen Tam (additional)
Laura Flynn (additional)
Teresa Vanni-Reyes (additional)
Michael D. Gunn (additional)
Ira J. Goldberg (additional)
Robert L. Hamilton (additional)
Stephen G. Young (additional)

References 13 Referenced 111

10.1161/circ.82.5.1977530
10.1016/S0962-8924(00)89054-6
10.1172/JCI116927
10.1073/pnas.91.6.2130
10.1074/jbc.271.20.11963
10.1074/jbc.272.47.29752
10.1007/BF01033631
10.1016/0005-2760(86)90084-6
10.1073/pnas.91.16.7628
Tahiliani AG. Myocardial fatty acid metabolism. In: Fozzard HA Haber E Jennings RB Katz AM Morgan HE eds. The Heart and Cardiovascular System: Scientific Foundations. 2nd ed. New York NY: Raven Press; 1992;2:1599–1640.
10.1152/physrev.1992.72.4.881
10.1097/00041433-199404000-00003
10.1016/S0022-2275(20)39976-4

Dates

Type	When
Created	13 years, 2 months ago (June 11, 2012, 8:41 p.m.)
Deposited	1 year, 3 months ago (May 12, 2024, 12:20 p.m.)
Indexed	1 month, 3 weeks ago (July 6, 2025, 1:45 p.m.)
Issued	27 years, 1 month ago (July 7, 1998)
Published	27 years, 1 month ago (July 7, 1998)
Published Print	27 years, 1 month ago (July 7, 1998)

Funders 0

None

BibTeX

@article{Nielsen_1998, title={Genes for Apolipoprotein B and Microsomal Triglyceride Transfer Protein Are Expressed in the Heart: Evidence That the Heart Has the Capacity to Synthesize and Secrete Lipoproteins}, volume={98}, ISSN={1524-4539}, url={http://dx.doi.org/10.1161/01.cir.98.1.13}, DOI={10.1161/01.cir.98.1.13}, number={1}, journal={Circulation}, publisher={Ovid Technologies (Wolters Kluwer Health)}, author={Nielsen, Lars B. and Véniant, Murielle and Borén, Jan and Raabe, Martin and Wong, Jinny S. and Tam, Carmen and Flynn, Laura and Vanni-Reyes, Teresa and Gunn, Michael D. and Goldberg, Ira J. and Hamilton, Robert L. and Young, Stephen G.}, year={1998}, month=jul, pages={13–16} }

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  "abstract": "<jats:p>\n            <jats:italic>Background</jats:italic>\n            \u2014Expression of both the apolipoprotein B (apoB) gene and the microsomal triglyceride transfer protein (MTP) gene is required for the assembly and secretion of triglyceride-rich lipoproteins in the liver and intestine. Both genes have been assumed to be silent in the heart.\n          </jats:p>\n          <jats:p>\n            <jats:italic>Methods and Results</jats:italic>\n            \u2014Northern blot and RNase protection analyses showed that the apoB and MTP genes were expressed in the hearts of mice and humans. In situ hybridization studies revealed that the apoB mRNA was produced in cardiac myocytes. Electron microscopy of human cardiac myocytes revealed lipid-staining particles of relatively small diameter (\u2248250 A\u030a) within the Golgi apparatus.\n          </jats:p>\n          <jats:p>\n            <jats:italic>Conclusions</jats:italic>\n            \u2014These studies strongly suggest that the heart synthesizes and secretes apoB-containing lipoproteins.\n          </jats:p>",
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