DOI: 10.1161/01.cir.95.4.1030. Vascular Endothelial Growth Factor/Vascular Permeability Factor Augments Nitric Oxide Release From Quiescent Rabbit and Human Vascular Endothelium

Vascular Endothelial Growth Factor/Vascular Permeability Factor Augments Nitric Oxide Release From Quiescent Rabbit and Human Vascular Endothelium

10.1161/01.cir.95.4.1030

Crossref journal-article

Ovid Technologies (Wolters Kluwer Health)

Circulation (276)

Abstract

Background Vascular endothelial growth factor (VEGF)/vascular permeability factor (VPF) is an endothelial cell (EC) mitogen. This feature is considered central to the documented role of VEGF/VPF in promoting angiogenesis. More recent evidence suggests that VEGF/VPF may also serve a “maintenance” function, modulating various aspects of EC biology. In the present study, we sought to determine the extent to which VEGF/VPF may stimulate the release of NO from normal ECs. Methods and Results VEGF/VPF produced a dose-dependent rise in NO concentration ([NO]) from vascular segments of rabbit thoracic aorta, pulmonary artery, and inferior vena cava. In comparison to stimulation with acetylcholine, the onset of increased [NO] after administration of VEGF/VPF was slower, reaching a maximum value after 8 minutes. Preincubation of the aortic segments with l -arginine raised by twofold both baseline [NO] and [NO] stimulated by addition of 2.5 μg/mL VEGF/VPF. Removal of CaCl 2 from the Krebs solution, disruption of the endothelium, and administration of N G -monomethyl- l -arginine abrogated the stimulatory effect of 10 μg/mL VEGF/VPF. Similar findings were documented with an NO-specific polarographic electrode to measure NO released from cultured human umbilical vein ECs. Conclusions VEGF/VPF stimulates production of NO from rabbit and human ECs. This finding (1) constitutes inferential evidence for the presence of functional VEGF/VPF receptors on quiescent endothelium of the adult rabbit as well as human ECs and (2) supports the notion that putative maintenance functions of VEGF/VPF may include regulation of baseline synthesis and/or release of EC NO.

Bibliography

van der Zee, R., Murohara, T., Luo, Z., Zollmann, F., Passeri, J., Lekutat, C., & Isner, J. M. (1997). Vascular Endothelial Growth Factor/Vascular Permeability Factor Augments Nitric Oxide Release From Quiescent Rabbit and Human Vascular Endothelium. Circulation, 95(4), 1030â1037.

Authors 7

Rien van der Zee (first)
Toyoaki Murohara (additional)
Zhengyu Luo (additional)
Frank Zollmann (additional)
Jonathan Passeri (additional)
Carsten Lekutat (additional)
Jeffrey M. Isner (additional)

References 48 Referenced 289

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Dates

Type	When
Created	13 years, 2 months ago (June 11, 2012, 8:41 p.m.)
Deposited	1 year, 3 months ago (May 12, 2024, 12:54 p.m.)
Indexed	2 weeks ago (Aug. 23, 2025, 1:08 a.m.)
Issued	28 years, 6 months ago (Feb. 18, 1997)
Published	28 years, 6 months ago (Feb. 18, 1997)
Published Print	28 years, 6 months ago (Feb. 18, 1997)

Funders 0

None

BibTeX

@article{van_der_Zee_1997, title={Vascular Endothelial Growth Factor/Vascular Permeability Factor Augments Nitric Oxide Release From Quiescent Rabbit and Human Vascular Endothelium}, volume={95}, ISSN={1524-4539}, url={http://dx.doi.org/10.1161/01.cir.95.4.1030}, DOI={10.1161/01.cir.95.4.1030}, number={4}, journal={Circulation}, publisher={Ovid Technologies (Wolters Kluwer Health)}, author={van der Zee, Rien and Murohara, Toyoaki and Luo, Zhengyu and Zollmann, Frank and Passeri, Jonathan and Lekutat, Carsten and Isner, Jeffrey M.}, year={1997}, month=feb, pages={1030–1037} }

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