DOI: 10.1161/01.cir.94.1.19. Modulation of Oxidant Stress In Vivo in Chronic Cigarette Smokers [Penn]

Modulation of Oxidant Stress In Vivo in Chronic Cigarette Smokers

10.1161/01.cir.94.1.19

Crossref journal-article

Ovid Technologies (Wolters Kluwer Health)

Circulation (276)

Abstract

BackgroundFree radical–induced oxidative damage is thought to be involved in the pathogenesis of diseases associated with cigarette smoking. We examined the production of 8-epi-prostaglandin (PG) F2α, a stable product of lipid peroxidation in vivo, and its modulation by aspirin and antioxidant vitamins in chronic cigarette smokers.Methods and ResultsWe performed the following studies: (1) a cross-sectional comparison of smokers and control subjects, (2) an examination of the dose-response relationship, (3) an exploration of the effect of smoking cessation (3 weeks) and nicotine patch supplementation, (4) the effect of aspirin consumption, and (5) the effects of 5 days’ dosing with vitamin E (100 and 800 U), vitamin C (2 g), and their combination. 8-epi-PGF2αexcretion (in pmol/mmol, mean±SEM) was 176.5±30.6 in heavy smokers, 92.7±4.8 (P<.05) in moderate smokers, and 54.1±2.7 (P<.005) in nonsmokers. Urinary levels fell from 145.5±24.9 to 114.6±27.1 (week 2,P<.05) and 112.6±24.9 (week 3,P<.05) on cessation of smoking. Aspirin treatment failed to suppress urinary levels of 8-epi-PGF2αdespite a significant reduction in urinary 11-dehydro-TxB2production and suppression of 8-epi-PGF2αand TxB2in serum. Vitamin C (pre, 194.6±40.9; post, 137.2±34.1;P<.05) and a combination of vitamin C and E (pre, 171.0±39.8; post, 133.5±29.6;P<.05) suppressed urinary 8-epi-PGF2α, whereas vitamin E alone had no effect.ConclusionsUrinary 8-epi-PGF2αmay represent a noninvasive, quantitative index of oxidant stress in vivo. Elevated levels of 8-epi-PGF2αin smokers may be modulated by quitting cigarettes and switching to nicotine patches or by antioxidant vitamin therapy.

Bibliography

Reilly, M., Delanty, N., Lawson, J. A., & FitzGerald, G. A. (1996). Modulation of Oxidant Stress In Vivo in Chronic Cigarette Smokers. Circulation, 94(1), 19â25.

Authors 4

Murdeach Reilly (first)
Norman Delanty (additional)
John A. Lawson (additional)
Garret A. FitzGerald (additional)

References 56 Referenced 443

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Dates

Type	When
Created	13 years, 2 months ago (June 11, 2012, 8:34 p.m.)
Deposited	5 months ago (March 30, 2025, 3:53 p.m.)
Indexed	1 week, 1 day ago (Aug. 29, 2025, 5:42 a.m.)
Issued	29 years, 2 months ago (July 1, 1996)
Published	29 years, 2 months ago (July 1, 1996)
Published Print	29 years, 2 months ago (July 1, 1996)

Funders 0

None

BibTeX

@article{Reilly_1996, title={Modulation of Oxidant Stress In Vivo in Chronic Cigarette Smokers}, volume={94}, ISSN={1524-4539}, url={http://dx.doi.org/10.1161/01.cir.94.1.19}, DOI={10.1161/01.cir.94.1.19}, number={1}, journal={Circulation}, publisher={Ovid Technologies (Wolters Kluwer Health)}, author={Reilly, Murdeach and Delanty, Norman and Lawson, John A. and FitzGerald, Garret A.}, year={1996}, month=jul, pages={19–25} }

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