DOI: 10.1161/01.cir.93.8.1493. Assignment of the Vascular Endothelial Growth Factor Gene to Human Chromosome 6p21.3

Assignment of the Vascular Endothelial Growth Factor Gene to Human Chromosome 6p21.3

10.1161/01.cir.93.8.1493

Crossref journal-article

Ovid Technologies (Wolters Kluwer Health)

Circulation (276)

Abstract

Background Vascular endothelial growth factor (VEGF) is an endothelial cell–specific growth factor and a regulator of physiological and pathological angiogenesis. Four different proteins are produced by alternative splicing of a unique transcript generated from a single-copy gene. Knowledge of the chromosomal location of the VEGF gene would help in determining a linkage to any known human congenital syndrome and/or to known chromosomal rearrangements in tumors. Methods and Results A human chromosome mapping panel was used to assign the VEGF gene to human chromosomes by polymerase chain reaction using VEGF -specific oligonucleotide primers. Amplified DNA fragments were fractionated on a 1% agarose gel. A single band of the expected size was obtained only from the DNA of those hybrid cell lines that contained the human chromosome 6. Three YAC clones containing the VEGF gene were obtained by screening the ICI Diagnostics library. In situ hybridization was then used to locate the VEGF gene in the 6p21.3 region. Conclusions The location of the VEGF gene in the 6p21.3 region is a potential starting point for a linkage study. In addition, the isolation of YAC clones containing the VEGF gene will contribute to the construction of the physical map of this chromosomal region.

Bibliography

Vincenti, V., Cassano, C., Rocchi, M., & Persico, M. G. (1996). Assignment of the Vascular Endothelial Growth Factor Gene to Human Chromosome 6p21.3. Circulation, 93(8), 1493â1495.

Authors 4

Valeria Vincenti (first)
Caterina Cassano (additional)
Mariano Rocchi (additional)
M. Graziella Persico (additional)

References 17 Referenced 347

10.1126/science.2479987
10.1126/science.2479986
10.1016/S0021-9258(18)99049-6
10.1126/science.1312256
10.3109/08977199409046916
Hashimoto E, Ogita T, Nakaoka T, Matsuoka R, Takao A, Kira Y. Rapid induction of vascular endothelial growth factor expression by transient ischemia in rat heart. Am J Physiol. 1994;267:1948-1954. / Am J Physiol (1994)
10.1172/JCI117018
10.3109/08977199409000240
Maglione D, Guerriero V, Viglietto G, Ferraro MG, Aprelikova O, Alitalo K, Del Vecchio S, Lei K-J, Chou JY, Persico MG. Two alternative mRNAs coding for the angiogenic factor, placenta growth factor (PlGF), are transcribed from a single gene of chromosome 14. Oncogene. 1993;8:925-931. / Oncogene (1993)
10.1093/nar/17.9.3425
DiPalma T Tucci M Russo G Maglione D Lago CT Romano A Saccone S DellaValle G De Gregorio L Dragani TA Viglietto G Persico MG. The placenta growth factor gene of the mouse. Mamm Genome. In press.
10.1073/pnas.88.20.9267
10.1038/376062a0
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10.1006/geno.1994.1302
10.1006/geno.1994.1405
Rosnet O, Stephenson D, Mattei M-G, Marchetto S, Shibuya M, Chapman VM, Binbaum D. Close physical linkage of the FLT1 and FLT3 genes on chromosome 13 in man and chromosome 5 in mouse. Oncogene. 1993;8:173-179. / Oncogene (1993)

Dates

Type	When
Created	13 years, 2 months ago (June 11, 2012, 8:34 p.m.)
Deposited	1 year, 3 months ago (May 12, 2024, 11:59 a.m.)
Indexed	4 days, 4 hours ago (Sept. 2, 2025, 6:44 a.m.)
Issued	29 years, 4 months ago (April 15, 1996)
Published	29 years, 4 months ago (April 15, 1996)
Published Print	29 years, 4 months ago (April 15, 1996)

Funders 0

None

BibTeX

@article{Vincenti_1996, title={Assignment of the Vascular Endothelial Growth Factor Gene to Human Chromosome 6p21.3}, volume={93}, ISSN={1524-4539}, url={http://dx.doi.org/10.1161/01.cir.93.8.1493}, DOI={10.1161/01.cir.93.8.1493}, number={8}, journal={Circulation}, publisher={Ovid Technologies (Wolters Kluwer Health)}, author={Vincenti, Valeria and Cassano, Caterina and Rocchi, Mariano and Persico, M. Graziella}, year={1996}, month=apr, pages={1493–1495} }

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