DOI: 10.1161/01.cir.103.22.2731. Cardiac-Specific LIM Protein FHL2 Modifies the Hypertrophic Response to β-Adrenergic Stimulation

Cardiac-Specific LIM Protein FHL2 Modifies the Hypertrophic Response to β-Adrenergic Stimulation

10.1161/01.cir.103.22.2731

Crossref journal-article

Ovid Technologies (Wolters Kluwer Health)

Circulation (276)

Abstract

Background —A deficiency of muscle LIM protein results in dilated cardiomyopathy, but the function of other LIM proteins in the heart has not been assessed previously. We have characterized the expression and function of FHL2, a heart-specific member of the LIM domain gene family. Methods and Results —Expression of FHL2 mRNA and protein was examined by Northern blot, in situ hybridization, and Western blot analyses of fetal and adult mice. FHL2 transcripts are present at embryonic day (E) 7.5 within the cardiac crescent in a pattern that resembles that of Nkx2.5 mRNA. During later stages of cardiac development and in adult animals, FHL2 expression is localized to the myocardium and absent from endocardium, cardiac cushion, outflow tract, or coronary vasculature. The gene encoding FHL2 was disrupted by homologous recombination, and knockout mice devoid of FHL2 were found to undergo normal cardiovascular development. In the absence of FHL2, however, cardiac hypertrophy resulting from chronic infusion of isoproterenol is exaggerated (59% versus 20% increase in heart weight/body weight in FHL null versus wild-type mice; P <0.01). Conclusions —FHL2 is an early marker of cardiogenic cells and a cardiac-specific LIM protein in the adult. FHL2 is not required for normal cardiac development but modifies the hypertrophic response to β-adrenergic stimulation.

Bibliography

Kong, Y., Shelton, J. M., Rothermel, B., Li, X., Richardson, J. A., Bassel-Duby, R., & Williams, R. S. (2001). Cardiac-Specific LIM Protein FHL2 Modifies the Hypertrophic Response to Î²-Adrenergic Stimulation. Circulation, 103(22), 2731â2738.

Authors 7

Yanfeng Kong (first)
John M. Shelton (additional)
Beverly Rothermel (additional)
Xiangqing Li (additional)
James A. Richardson (additional)
Rhonda Bassel-Duby (additional)
R. Sanders Williams (additional)

References 33 Referenced 123

Jurata LW, Gill GN. Structure and function of LIM domains. Curr Top Microbiol Immunol. 1998;228:75–113. / Curr Top Microbiol Immunol (1998)
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Dates

Type	When
Created	13 years, 2 months ago (June 11, 2012, 8:09 p.m.)
Deposited	1 year, 3 months ago (May 12, 2024, 8:58 a.m.)
Indexed	1 week, 4 days ago (Aug. 19, 2025, 6:07 a.m.)
Issued	24 years, 2 months ago (June 5, 2001)
Published	24 years, 2 months ago (June 5, 2001)
Published Print	24 years, 2 months ago (June 5, 2001)

Funders 0

None

BibTeX

@article{Kong_2001, title={Cardiac-Specific LIM Protein FHL2 Modifies the Hypertrophic Response to β-Adrenergic Stimulation}, volume={103}, ISSN={1524-4539}, url={http://dx.doi.org/10.1161/01.cir.103.22.2731}, DOI={10.1161/01.cir.103.22.2731}, number={22}, journal={Circulation}, publisher={Ovid Technologies (Wolters Kluwer Health)}, author={Kong, Yanfeng and Shelton, John M. and Rothermel, Beverly and Li, Xiangqing and Richardson, James A. and Bassel-Duby, Rhonda and Williams, R. Sanders}, year={2001}, month=jun, pages={2731–2738} }

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  "abstract": "<jats:p>\n            <jats:italic>Background</jats:italic>\n            \u2014A deficiency of muscle LIM protein results in dilated cardiomyopathy, but the function of other LIM proteins in the heart has not been assessed previously. We have characterized the expression and function of FHL2, a heart-specific member of the LIM domain gene family.\n          </jats:p>\n          <jats:p>\n            <jats:italic>Methods and Results</jats:italic>\n            \u2014Expression of FHL2 mRNA and protein was examined by Northern blot, in situ hybridization, and Western blot analyses of fetal and adult mice. FHL2 transcripts are present at embryonic day (E) 7.5 within the cardiac crescent in a pattern that resembles that of Nkx2.5 mRNA. During later stages of cardiac development and in adult animals, FHL2 expression is localized to the myocardium and absent from endocardium, cardiac cushion, outflow tract, or coronary vasculature. The gene encoding FHL2 was disrupted by homologous recombination, and knockout mice devoid of FHL2 were found to undergo normal cardiovascular development. In the absence of FHL2, however, cardiac hypertrophy resulting from chronic infusion of isoproterenol is exaggerated (59% versus 20% increase in heart weight/body weight in FHL null versus wild-type mice;\n            <jats:italic>P</jats:italic>\n            &lt;0.01).\n          </jats:p>\n          <jats:p>\n            <jats:italic>Conclusions</jats:italic>\n            \u2014FHL2 is an early marker of cardiogenic cells and a cardiac-specific LIM protein in the adult. FHL2 is not required for normal cardiac development but modifies the hypertrophic response to \u03b2-adrenergic stimulation.\n          </jats:p>",
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