DOI: 10.1161/01.cir.0000070931.62772.4e. In Vivo Magnetic Resonance Imaging of Mesenchymal Stem Cells in Myocardial Infarction

In Vivo Magnetic Resonance Imaging of Mesenchymal Stem Cells in Myocardial Infarction

10.1161/01.cir.0000070931.62772.4e

Crossref journal-article

Ovid Technologies (Wolters Kluwer Health)

Circulation (276)

Abstract

Background— We investigated the potential of magnetic resonance imaging (MRI) to track magnetically labeled mesenchymal stem cells (MR-MSCs) in a swine myocardial infarction (MI) model. Methods and Results— Adult farm pigs (n=5) were subjected to closed-chest experimental MI. MR-MSCs (2.8 to 16×10 7 cells) were injected intramyocardially under x-ray fluoroscopy. MRIs were obtained on a 1.5T MR scanner to demonstrate the location of the MR-MSCs and were correlated with histology. Contrast-enhanced MRI demonstrated successful injection in the infarct and serial MSC tracking was demonstrated in two animals. Conclusion— MRI tracking of MSCs is feasible and represents a preferred method for studying the engraftment of MSCs in MI.

Bibliography

Kraitchman, D. L., Heldman, A. W., Atalar, E., Amado, L. C., Martin, B. J., Pittenger, M. F., Hare, J. M., & Bulte, J. W. M. (2003). In Vivo Magnetic Resonance Imaging of Mesenchymal Stem Cells in Myocardial Infarction. Circulation, 107(18), 2290â2293.

Authors 8

Dara L. Kraitchman (first)
Alan W. Heldman (additional)
Ergin Atalar (additional)
Luciano C. Amado (additional)
Bradley J. Martin (additional)
Mark F. Pittenger (additional)
Joshua M. Hare (additional)
Jeff W.M. Bulte (additional)

References 10 Referenced 579

10.1172/JCI12150
10.1067/mtc.2000.110250
10.1038/35070587
10.1161/circ.105.1.93
10.1038/86498
10.1038/nbt1201-1141
10.1126/science.284.5411.143
Frank JA Miller BA Arbab AS et al. Clinically applicable labeling of mammalian (stem) cells by combining (FDA)-approved superparamagnetic iron oxides and commonly available transfection agents. Radiology . In press.
10.1016/S0003-4975(02)03517-8
10.1161/01.cir.0000034046.87607.1c

Dates

Type	When
Created	22 years, 3 months ago (May 12, 2003, 6:33 p.m.)
Deposited	1 year, 3 months ago (May 6, 2024, 2:36 p.m.)
Indexed	3 weeks, 5 days ago (Aug. 2, 2025, 12:12 a.m.)
Issued	22 years, 3 months ago (May 13, 2003)
Published	22 years, 3 months ago (May 13, 2003)
Published Print	22 years, 3 months ago (May 13, 2003)

Funders 0

None

BibTeX

@article{Kraitchman_2003, title={In Vivo Magnetic Resonance Imaging of Mesenchymal Stem Cells in Myocardial Infarction}, volume={107}, ISSN={1524-4539}, url={http://dx.doi.org/10.1161/01.cir.0000070931.62772.4e}, DOI={10.1161/01.cir.0000070931.62772.4e}, number={18}, journal={Circulation}, publisher={Ovid Technologies (Wolters Kluwer Health)}, author={Kraitchman, Dara L. and Heldman, Alan W. and Atalar, Ergin and Amado, Luciano C. and Martin, Bradley J. and Pittenger, Mark F. and Hare, Joshua M. and Bulte, Jeff W.M.}, year={2003}, month=may, pages={2290–2293} }

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  "abstract": "<jats:p>\n            <jats:bold>\n              <jats:italic>Background\u2014</jats:italic>\n            </jats:bold>\n            We investigated the potential of magnetic resonance imaging (MRI) to track magnetically labeled mesenchymal stem cells (MR-MSCs) in a swine myocardial infarction (MI) model.\n          </jats:p>\n          <jats:p>\n            <jats:bold>\n              <jats:italic>Methods and Results\u2014</jats:italic>\n            </jats:bold>\n            Adult farm pigs (n=5) were subjected to closed-chest experimental MI. MR-MSCs (2.8 to 16\u00d710\n            <jats:sup>7</jats:sup>\n            cells) were injected intramyocardially under x-ray fluoroscopy. MRIs were obtained on a 1.5T MR scanner to demonstrate the location of the MR-MSCs and were correlated with histology. Contrast-enhanced MRI demonstrated successful injection in the infarct and serial MSC tracking was demonstrated in two animals.\n          </jats:p>\n          <jats:p>\n            <jats:bold>\n              <jats:italic>Conclusion\u2014</jats:italic>\n            </jats:bold>\n            MRI tracking of MSCs is feasible and represents a preferred method for studying the engraftment of MSCs in MI.\n          </jats:p>",
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