The occurrence and distribution of the vitamin-D-induced calcium-binding protein, calbindin-D<sub>28K</sub>, has been studied in the brain of a cartilaginous fish using immunohistochemical techniques. A strong immunoreactivity was found in the perikarya, dendrites and axons of neurons located in the nucleus interstitialis commissurae anterioris, the nucleus medialis of the left habenula, the thalamus dorsalis, the thalamus ventralis, the nucleus lobi lateralis, the nucleus interpeduncularis, the lobus vagi and the medial reticular zone. Fibre tracts associated with some of these neuronal groups, such as the fasciculus retroflexus, the stria medullaris and the commissura habenulae, also contained immunopositive fibres. Only a minor immunoreactivity could be detected in other brain areas such as the tectum mesencephali and some telencephalic zones. Interestingly, the cerebellum did not show any immunoreactivity in Purkinje cells nor in other neurons. The distribution of calbindin-D<sub>2</sub>8ĸ in the dogfish brain appears to be mainly related to the viscerosensory centres.

Rodriguez-Moldes, I., Timmermans, J. P., Adriaensen, D., De Groodt-Lasseel, M. H. A., Scheuermann, D. W., & Anadon, R. (1990). Immunohistochemical Localization of Calbindin-D₂₈_K in the Brain of a Cartilaginous Fish, the Dogfish (<i>Scyliorhinus canicula</i> L.). Cells Tissues Organs, 137(4), 293–302. Portico.