DOI: 10.1159/000133553. The uptake of uridine in the nucleolus occurs in the dense fibrillar component

The uptake of uridine in the nucleolus occurs in the dense fibrillar component

10.1159/000133553

Crossref journal-article

S. Karger AG

Cytogenetic and Genome Research (127)

Abstract

A new method was developed to localize the site of transcription within the nucleolus. Incorporation of digoxigenin-labeled UTP in nucleoli of human PHA-stimulated lymphocytes and in equine melanoma cells was visualized with antibodies against digoxigenin and secondary gold-labeled antibodies at the electron-microscopic level. This approach offers much higher spatial resolution than the autoradiographic methods used so far. In both types of cells digoxigenin-UTP was incorporated mainly into the dense fibrillar component; the fibrillar centers, in contrast, did not display significant labeling above background levels. This finding corroborates the view that the dense fibrillar component is the site of RNA transcription in the nucleolus.

Bibliography

SchÃ¶fer, C., MÃ¼ller, M., Leitner, M. D., & Wachtler, F. (1993). The uptake of uridine in the nucleolus occurs in the dense fibrillar component. Cytogenetic and Genome Research, 64(1), 27â30. Portico.

Authors 4

C. Schöfer (first)
M. Müller (additional)
M.D. Leitner (additional)
F. Wachtler (additional)

References 0 Referenced 9

None

Dates

Type	When
Created	17 years, 3 months ago (May 16, 2008, 8:10 a.m.)
Deposited	4 months, 1 week ago (April 23, 2025, 9:34 p.m.)
Indexed	4 months, 1 week ago (April 24, 2025, 12:54 a.m.)
Issued	32 years, 8 months ago (Jan. 1, 1993)
Published	32 years, 8 months ago (Jan. 1, 1993)
Published Online	17 years, 3 months ago (May 14, 2008)
Published Print	32 years, 8 months ago (Jan. 1, 1993)

Funders 0

None

BibTeX

@article{Sch_fer_1993, title={The uptake of uridine in the nucleolus occurs in the dense fibrillar component}, volume={64}, ISSN={1424-859X}, url={http://dx.doi.org/10.1159/000133553}, DOI={10.1159/000133553}, number={1}, journal={Cytogenetic and Genome Research}, publisher={S. Karger AG}, author={Schöfer, C. and Müller, M. and Leitner, M.D. and Wachtler, F.}, year={1993}, pages={27–30} }

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