DOI: 10.1159/000070925. Magnoliae Flos Induces Apoptosis of RBL-2H3 Cells via Mitochondria and Caspase

Magnoliae Flos Induces Apoptosis of RBL-2H3 Cells via Mitochondria and Caspase

10.1159/000070925

Crossref journal-article

S. Karger AG

International Archives of Allergy and Immunology (127)

Abstract

Background: Magnoliae flores (MF), the buds of Magnolia denudata Desrousseaux, have been successfully used for the management of allergic diseases in Korea. The purpose of the present study was to determine their causal role in inducing apoptosis of mast cells and to verify the underlying mechanism. Methods: The viability of mast cells was assessed by the trypan blue exclusion test. Induction of apoptosis was confirmed by DNA fragmentation, nuclear staining and DNA hypoploidy. Western blotting and immunofluorescent staining were performed to study the alterations in expression level and translocation of apoptosis-related proteins. Mitochondrial membrane potential (MMP) change and cytochrome C release were assayed. Results: We present several lines of evidence indicating that MF induce apoptosis. Changes in cell morphology, generation of DNA fragmentation, cell cycle arrest, activation of caspase-3, and PARP and DFF degradations were demonstrated. The reduction of MMP and the release of cytochrome C to cytosol were also shown. Either PTP blockers, bongkrekic acid and cyclosporin A, or pancaspase inhibitors, Boc.D-fmk and zVAD-fmk, did not prevent the release of cytochrome C. Bax protein content was increased, and Bax was translocated from cytosol into mitochondria at early time points after MF treatment. Conclusions: We have demonstrated that MF induce mitochondria- and caspase-dependent mast cell apoptosis. Our observations contribute new insights to the role of MF and support the view that the clinical effect of MF may depend on their pharmacological efficacy in regulating mast cell apoptosis.

Bibliography

Kim, G. C., Lee, S. G., Park, B. S., Kim, J. Y., Song, Y. S., Kim, J. M., Yoo, K. S., Huh, G. Y., Jeong, M. H., Lim, Y. J., Kim, H. M., & Yoo, Y. H. (2003). Magnoliae Flos Induces Apoptosis of RBL-2H3 Cells via Mitochondria and Caspase. International Archives of Allergy and Immunology, 131(2), 101â110. Portico.

Authors 12

Gyoo C. Kim (first)
Soon G. Lee (additional)
Bong S. Park (additional)
Jee Y. Kim (additional)
Yeon S. Song (additional)
Jong M. Kim (additional)
Ki S. Yoo (additional)
Gi Y. Huh (additional)
Min H. Jeong (additional)
Young Jin Lim (additional)
Hyung M. Kim (additional)
Young Hyun Yoo (additional)

References 14 Referenced 16

Dates

Type	When
Created	22 years, 2 months ago (June 20, 2003, 5:41 a.m.)
Deposited	4 months, 1 week ago (April 23, 2025, 11:25 p.m.)
Indexed	4 months, 1 week ago (April 25, 2025, 12:09 a.m.)
Issued	22 years, 8 months ago (Jan. 1, 2003)
Published	22 years, 8 months ago (Jan. 1, 2003)
Published Online	16 years, 1 month ago (July 17, 2009)
Published Print	22 years, 8 months ago (Jan. 1, 2003)

Funders 0

None

BibTeX

@article{Kim_2003, title={Magnoliae Flos Induces Apoptosis of RBL-2H3 Cells via Mitochondria and Caspase}, volume={131}, ISSN={1423-0097}, url={http://dx.doi.org/10.1159/000070925}, DOI={10.1159/000070925}, number={2}, journal={International Archives of Allergy and Immunology}, publisher={S. Karger AG}, author={Kim, Gyoo C. and Lee, Soon G. and Park, Bong S. and Kim, Jee Y. and Song, Yeon S. and Kim, Jong M. and Yoo, Ki S. and Huh, Gi Y. and Jeong, Min H. and Lim, Young Jin and Kim, Hyung M. and Yoo, Young Hyun}, year={2003}, pages={101–110} }

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  "abstract": "<jats:p>&lt;i&gt;Background:&lt;/i&gt; Magnoliae flores (MF), the buds of &lt;i&gt;Magnolia denudata Desrousseaux&lt;/i&gt;, have been successfully used for the management of allergic diseases in Korea. The purpose of the present study was to determine their causal role in inducing apoptosis of mast cells and to verify the underlying mechanism. &lt;i&gt;Methods:&lt;/i&gt; The viability of mast cells was assessed by the trypan blue exclusion test. Induction of apoptosis was confirmed by DNA fragmentation, nuclear staining and DNA hypoploidy. Western blotting and immunofluorescent staining were performed to study the alterations in expression level and translocation of apoptosis-related proteins. Mitochondrial membrane potential (MMP) change and cytochrome C release were assayed. &lt;i&gt;Results:&lt;/i&gt; We present several lines of evidence indicating that MF induce apoptosis. Changes in cell morphology, generation of DNA fragmentation, cell cycle arrest, activation of caspase-3, and PARP and DFF degradations were demonstrated. The reduction of MMP and the release of cytochrome C to cytosol were also shown. Either PTP blockers, bongkrekic acid and cyclosporin A, or pancaspase inhibitors, Boc.D-fmk and zVAD-fmk, did not prevent the release of cytochrome C. Bax protein content was increased, and Bax was translocated from cytosol into mitochondria at early time points after MF treatment. &lt;i&gt;Conclusions:&lt;/i&gt; We have demonstrated that MF induce mitochondria- and caspase-dependent mast cell apoptosis. Our observations contribute new insights to the role of MF and support the view that the clinical effect of MF may depend on their pharmacological efficacy in regulating mast cell apoptosis.</jats:p>",
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