DOI: 10.1159/000069458. Lactobacillus casei Inhibits Antigen-Induced IgE Secretion through Regulation of Cytokine Production in Murine Splenocyte Cultures

Lactobacillus casei Inhibits Antigen-Induced IgE Secretion through Regulation of Cytokine Production in Murine Splenocyte Cultures

10.1159/000069458

Crossref journal-article

S. Karger AG

International Archives of Allergy and Immunology (127)

Abstract

Background:Lactobacillus casei is a nonpathogenic gram-positive bacterium widely used in dairy products and has been shown to enhance the cellular immunity of the host. Methods: To examine the inhibitory effect of L. casei on IgE production, splenocytes obtained from ovalbumin (OVA)-primed BALB/c mice were restimulated in vitro with the same antigen in the presence of heat-killed L. casei. The effect of this bacterium on T helper (Th) phenotype development was also examined with naive T cells from OVA-specific T cell receptor-transgenic mice. Results:L. casei induced IFN-γ, but inhibited IL-4 and IL-5 secretion, and markedly suppressed total and antigen-specific IgE secretion by OVA-stimulated splenocytes. The inhibitory effect of L. casei on IgE, IL-4, and IL-5 production was partially abrogated by addition of neutralizing antibody to IFN-γ. Augmented IL-12 production was also observed in the cell cultures containing L. casei, and anti-IL-12 monoclonal antibody completely restored the IgE, IL-4, and IL-5 production to the control levels. The IL-12 augmentation by L. casei was macrophage-dependent. The Th cell development assay showed the ability of L. casei to induce Th1 development preferentially. This effect was also completely blocked by anti-IL-12 antibody. Conclusions: This is the first demonstration that a nonpathogenic microorganism, L. casei, can inhibit antigen-induced IgE production through induction of IL-12 secretion by macrophages. The findings suggest a potential use of this organism in preventing IgE-mediated allergy.

Bibliography

Shida, K., Makino, K., Morishita, A., Takamizawa, K., Hachimura, S., Ametani, A., Sato, T., Kumagai, Y., Habu, S., & Kaminogawa, S. (1998). Lactobacillus casei Inhibits Antigen-Induced IgE Secretion through Regulation of Cytokine Production in Murine Splenocyte Cultures. International Archives of Allergy and Immunology, 115(4), 278â287. Portico.

Authors 10

Kan Shida (first)
Kumiko Makino (additional)
Aki Morishita (additional)
Kotaro Takamizawa (additional)
Satoshi Hachimura (additional)
Akio Ametani (additional)
Takehito Sato (additional)
Yoshihiro Kumagai (additional)
Sonoko Habu (additional)
Shuichi Kaminogawa (additional)

References 3 Referenced 206

Dates

Type	When
Created	22 years, 4 months ago (April 15, 2003, 6:10 a.m.)
Deposited	4 months, 1 week ago (April 23, 2025, 11:45 p.m.)
Indexed	3 months, 2 weeks ago (May 18, 2025, 10:24 a.m.)
Issued	27 years, 8 months ago (Jan. 1, 1998)
Published	27 years, 8 months ago (Jan. 1, 1998)
Published Online	27 years, 5 months ago (April 2, 1998)
Published Print	27 years, 8 months ago (Jan. 1, 1998)

Funders 0

None

BibTeX

@article{Shida_1998, title={Lactobacillus casei Inhibits Antigen-Induced IgE Secretion through Regulation of Cytokine Production in Murine Splenocyte Cultures}, volume={115}, ISSN={1423-0097}, url={http://dx.doi.org/10.1159/000069458}, DOI={10.1159/000069458}, number={4}, journal={International Archives of Allergy and Immunology}, publisher={S. Karger AG}, author={Shida, Kan and Makino, Kumiko and Morishita, Aki and Takamizawa, Kotaro and Hachimura, Satoshi and Ametani, Akio and Sato, Takehito and Kumagai, Yoshihiro and Habu, Sonoko and Kaminogawa, Shuichi}, year={1998}, pages={278–287} }

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