DOI: 10.1159/000054345. Food Deprivation Decreases mRNA and Activity of the Rat Dopamine Transporter

Food Deprivation Decreases mRNA and Activity of the Rat Dopamine Transporter

10.1159/000054345

Crossref journal-article

S. Karger AG

Neuroendocrinology (127)

Abstract

We have hypothesized that the midbrain dopamine (DA) neurons are a target for insulin action in the central nervous system (CNS). In support of this hypothesis, we have previously demonstrated that direct intracerebroventricular infusion of insulin results in an increase in mRNA levels for the DA reuptake transporter (DAT). In this study, 24- to 36-hour food deprivation was used as a model of decreased CNS insulin levels, to test whether DAT mRNA levels, DAT protein concentration or DAT functional activity would be decreased. DAT mRNA levels, assessed by in situ hybridization, were significantly decreased in the ventral tegmental area/substantia nigra pars compacta (VTA/SNc) (77 ± 7% of controls, p < 0.05) of food-deprived (hypoinsulinemic) rats. Binding of a specific high-affinity DAT ligand (125I-RTI-121) to membranes from brain regions of fasted or free-feeding rats provided an estimate of DAT protein, which was unchanged in both of the major terminal projection fields, the striatum and nucleus accumbens (NAc). In addition, we utilized the rotating disk electrode voltametry technique to assess possible changes in the function of the DAT in fasting (hypoinsulinemic) rats. The Vmax of DA uptake was significantly decreased (87 ± 7% of control, p < 0.05), without a change in the Km of uptake, in striatum from fasted rats. In vitro incubation with a physiological concentration (1 nM) of insulin resulted in an increase of striatal DA uptake to control levels. We conclude that striatal DAT function can be modulated by fasting and nutritional status, with a contribution by insulin.

Bibliography

Patterson, T. A., Brot, M. D., Zavosh, A., Schenk, J. O., Szot, P., & Figlewicz, D. P. (1998). Food Deprivation Decreases mRNA and Activity of the Rat Dopamine Transporter. Neuroendocrinology, 68(1), 11â20. Portico.

Authors 6

Terrell Ann Patterson (first)
Michelle D. Brot (additional)
Aryana Zavosh (additional)
James O. Schenk (additional)
Patricia Szot (additional)
Dianne P. Figlewicz (additional)

References 13 Referenced 128

Dates

Type	When
Created	22 years, 4 months ago (April 15, 2003, 3:06 p.m.)
Deposited	4 months, 1 week ago (April 24, 2025, 12:25 a.m.)
Indexed	6 days, 14 hours ago (Aug. 30, 2025, 12:49 p.m.)
Issued	27 years, 8 months ago (Jan. 1, 1998)
Published	27 years, 8 months ago (Jan. 1, 1998)
Published Online	17 years, 5 months ago (March 19, 2008)
Published Print	27 years, 8 months ago (Jan. 1, 1998)

Funders 0

None

BibTeX

@article{Patterson_1998, title={Food Deprivation Decreases mRNA and Activity of the Rat Dopamine Transporter}, volume={68}, ISSN={1423-0194}, url={http://dx.doi.org/10.1159/000054345}, DOI={10.1159/000054345}, number={1}, journal={Neuroendocrinology}, publisher={S. Karger AG}, author={Patterson, Terrell Ann and Brot, Michelle D. and Zavosh, Aryana and Schenk, James O. and Szot, Patricia and Figlewicz, Dianne P.}, year={1998}, pages={11–20} }

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