DOI: 10.1158/0008-5472.can-07-2580. Multiple Alternative Splicing Markers for Ovarian Cancer

Multiple Alternative Splicing Markers for Ovarian Cancer

10.1158/0008-5472.can-07-2580

Crossref journal-article

American Association for Cancer Research (AACR)

Cancer Research (1086)

Abstract

Abstract Intense efforts are currently being directed toward profiling gene expression in the hope of developing better cancer markers and identifying potential drug targets. Here, we present a sensitive new approach for the identification of cancer signatures based on direct high-throughput reverse transcription-PCR validation of alternative splicing events. This layered and integrated system for splicing annotation (LISA) fills a gap between high-throughput microarray studies and high-sensitivity individual gene investigations, and was created to monitor the splicing of 600 cancer-associated genes in 25 normal and 21 serous ovarian cancer tissues. Out of &gt;4,700 alternative splicing events screened, the LISA identified 48 events that were significantly associated with serous ovarian tumor tissues. In a further screen directed at 39 ovarian tissues containing cancer pathologies of various origins, our ovarian cancer splicing signature successfully distinguished all normal tissues from cancer. High-volume identification of cancer-associated splice forms by the LISA paves the way for the use of alternative splicing profiling to diagnose subtypes of cancer. [Cancer Res 2008;68(3):657–63]

Bibliography

Klinck, R., Bramard, A., Inkel, L., Dufresne-Martin, G., Gervais-Bird, J., Madden, R., Paquet, E. R., Koh, C., Venables, J. P., Prinos, P., Jilaveanu-Pelmus, M., Wellinger, R., Rancourt, C., Chabot, B., & Abou Elela, S. (2008). Multiple Alternative Splicing Markers for Ovarian Cancer. Cancer Research, 68(3), 657â663.

Authors 15

Roscoe Klinck (first)
Anne Bramard (additional)
Lyna Inkel (additional)
Geneviève Dufresne-Martin (additional)
Julien Gervais-Bird (additional)
Richard Madden (additional)
Éric R. Paquet (additional)
ChuShin Koh (additional)
Julian P. Venables (additional)
Panagiotis Prinos (additional)
Manuela Jilaveanu-Pelmus (additional)
Raymund Wellinger (additional)
Claudine Rancourt (additional)
Benoit Chabot (additional)
Sherif Abou Elela (additional)

References 48 Referenced 128

Dates

Type	When
Created	17 years, 6 months ago (Feb. 1, 2008, 2:04 p.m.)
Deposited	3 years, 2 months ago (June 17, 2022, 1:03 a.m.)
Indexed	1 month, 4 weeks ago (July 2, 2025, 2:13 p.m.)
Issued	17 years, 6 months ago (Feb. 1, 2008)
Published	17 years, 6 months ago (Feb. 1, 2008)
Published Online	17 years, 6 months ago (Feb. 1, 2008)
Published Print	17 years, 6 months ago (Feb. 1, 2008)

Funders 0

None

BibTeX

@article{Klinck_2008, title={Multiple Alternative Splicing Markers for Ovarian Cancer}, volume={68}, ISSN={1538-7445}, url={http://dx.doi.org/10.1158/0008-5472.can-07-2580}, DOI={10.1158/0008-5472.can-07-2580}, number={3}, journal={Cancer Research}, publisher={American Association for Cancer Research (AACR)}, author={Klinck, Roscoe and Bramard, Anne and Inkel, Lyna and Dufresne-Martin, Geneviève and Gervais-Bird, Julien and Madden, Richard and Paquet, Éric R. and Koh, ChuShin and Venables, Julian P. and Prinos, Panagiotis and Jilaveanu-Pelmus, Manuela and Wellinger, Raymund and Rancourt, Claudine and Chabot, Benoit and Abou Elela, Sherif}, year={2008}, month=feb, pages={657–663} }

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