DOI: 10.1155/s1110724302204027. Recombinational DNA Repair in Cancer and Normal Cells: The Challenge of Functional Analysis

Recombinational DNA Repair in Cancer and Normal Cells: The Challenge of Functional Analysis

10.1155/s1110724302204027

Crossref journal-article

Wiley

BioMed Research International (311)

Abstract

A major goal of current cancer research is to understand the functional consequences of mutations in recombinational DNA repair genes. The introduction of artificial recombination substrates into living cells has evolved into a powerful tool to perform functional analysis of DNA double strand break (DSB) repair. Here, we review the principles and practice of current plasmid assays with regard to the two major DSB repair pathways, homologous recombination and nonhomologous end‐joining. A spectrum of assay types is available to assess repair in a wide variety of cell lines. However, several technical challenges still need to be overcome. Understanding the alterations of DSB repair in cancers will ultimately provide a rational basis for drug design that may selectively sensitize tumor cells to ionizing radiation and chemotherapy, thereby achieving therapeutic gain.

Bibliography

Willers, H., Xia, F., & Powell, S. N. (2002). Recombinational DNA Repair in Cancer and Normal Cells: The Challenge of Functional Analysis. BioMed Research International, 2(2), 86â93. Portico.

Authors 3

Henning Willers (first)
Fen Xia (additional)
Simon N. Powell (additional)

References 0 Referenced 13

None

Dates

Type	When
Created	22 years, 10 months ago (Sept. 29, 2002, 2:36 p.m.)
Deposited	1 year ago (July 30, 2024, 2:20 a.m.)
Indexed	1 year ago (July 30, 2024, 2:40 a.m.)
Issued	23 years, 7 months ago (Jan. 1, 2002)
Published	23 years, 7 months ago (Jan. 1, 2002)
Published Online	22 years, 8 months ago (Dec. 10, 2002)
Published Print	23 years, 7 months ago (Jan. 1, 2002)

Funders 1

National Cancer Institute 10.13039/100000054
Region: Americas
gov (National government)
Labels4
1. Instituto Nacional del Cáncer
2. National Cancer Institute at the National Institutes of Health
3. Instituto Nacional del Cáncer de los Institutos Nacionales de la Salud
4. NCI

BibTeX

@article{Willers_2002, title={Recombinational DNA Repair in Cancer and Normal Cells: The Challenge of Functional Analysis}, volume={2}, ISSN={2314-6141}, url={http://dx.doi.org/10.1155/s1110724302204027}, DOI={10.1155/s1110724302204027}, number={2}, journal={BioMed Research International}, publisher={Wiley}, author={Willers, Henning and Xia, Fen and Powell, Simon N.}, year={2002}, month=jan, pages={86–93} }

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