DOI: 10.1155/2004/376572. Electrospray ion mobility spectrometry of intact viruses

Electrospray ion mobility spectrometry of intact viruses

10.1155/2004/376572

Crossref journal-article

Wiley

Journal of Spectroscopy (311)

Abstract

Characterizing supramolecular interactions offers significant challenges using NMR or crystallographic techniques either because of size limitations or the difficulty in forming suitable crystals, while mass spectrometry is largely limited to low resolution mass information. Here we report gas phase measurements of intact virus particles using electrospray ion mobility spectrometry with an accuracy in radial measurements that were sufficient to differentiate closely related species. In addition, measured diameters indicate that iscosahedral virus particles retain their structure in the gas phase as well as undergoing a slight compaction in the absence of solvent. Analysis of the human pathogen adenovirus represents the largest and most sophisticated biomolecular complex detected in the gas phase to date. These results, on a diverse set of viral systems, suggest that ion mobility spectrometry may have broad applications for the analysis of biological complexes.

Bibliography

Thomas, J. J., Bothner, B., Traina, J., Benner, W. H., & Siuzdak, G. (2004). Electrospray ion mobility spectrometry of intact viruses. Journal of Spectroscopy, 18(1), 31â36. Portico.

Authors 5

John J. Thomas (first)
Brian Bothner (additional)
Joe Traina (additional)
W. Henry Benner (additional)
Gary Siuzdak (additional)

References 0 Referenced 72

None

Dates

Type	When
Created	13 years, 5 months ago (March 6, 2012, 4:03 p.m.)
Deposited	1 year ago (Aug. 7, 2024, 8:25 p.m.)
Indexed	4 months, 2 weeks ago (April 8, 2025, 10:20 p.m.)
Issued	21 years, 7 months ago (Jan. 1, 2004)
Published	21 years, 7 months ago (Jan. 1, 2004)
Published Online	21 years, 7 months ago (Jan. 1, 2004)
Published Print	21 years, 7 months ago (Jan. 1, 2004)

Funders 1

National Institutes of Health 10.13039/100000002
Region: Americas
gov (National government)
Labels3
1. Institutos Nacionales de la Salud
2. US National Institutes of Health
3. NIH
Awards1
1. GM055775

BibTeX

@article{Thomas_2004, title={Electrospray ion mobility spectrometry of intact viruses}, volume={18}, ISSN={2314-4939}, url={http://dx.doi.org/10.1155/2004/376572}, DOI={10.1155/2004/376572}, number={1}, journal={Journal of Spectroscopy}, publisher={Wiley}, author={Thomas, John J. and Bothner, Brian and Traina, Joe and Benner, W. Henry and Siuzdak, Gary}, year={2004}, month=jan, pages={31–36} }

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