DOI: 10.1152/ajprenal.2001.281.1.f103. Effects of filtration rate on the glomerular barrier and clearance of four differently shaped molecules

Effects of filtration rate on the glomerular barrier and clearance of four differently shaped molecules

10.1152/ajprenal.2001.281.1.f103

Crossref journal-article

American Physiological Society

American Journal of Physiology-Renal Physiology (24)

Abstract

The effect of shape on the transglomerular passage of solutes has not been hitherto systematically studied. We perfused isolated rat kidneys to determine the fractional clearances (θ) at various filtration rates for four molecules of different shapes but with similar Stokes-Einstein radii ( aSE= 34–36 Å). The θ for hyaluronan, bikunin, and Ficoll36 Åwere 66, 16, and 11%, respectively, at a glomerular filtration rate (GFR) of 0.07 ml · min−1· g wet wt−1and decreased to 46, 14, and 7%, respectively, on a fivefold increase in GFR. Under the same conditions, θ for albumin increased from 0.15 to 0.74%, and similar behavior was observed for larger Ficolls ( aSE>45 Å). Pore analysis showed that the “apparent neutral” solute radii of Ficoll, albumin, bikunin, and hyaluronan were 35, 64, 33, and 24 Å, respectively, despite similar aSE. In addition, the properties of the glomerular filter changed with increasing GFR and hydrostatic pressure. We conclude that elongated shape, irrespective of size and charge, drastically increases the transglomerular passage of a solute, an effect that is related to its frictional ratio.

Bibliography

Ohlson, M., SÃ¶rensson, J., LindstrÃ¶m, K., Blom, A. M., Fries, E., & Haraldsson, B. (2001). Effects of filtration rate on the glomerular barrier and clearance of four differently shaped molecules. American Journal of Physiology-Renal Physiology, 281(1), F103âF113.

Authors 6

Maria Ohlson (first)
Jenny Sörensson (additional)
Karin Lindström (additional)
Anna M. Blom (additional)
Erik Fries (additional)
Börje Haraldsson (additional)

References 43 Referenced 73

10.1152/ajprenal.1997.273.3.F430
10.1172/JCI108927
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10.1038/ki.1982.229
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{'key': 'B10', 'first-page': '2614', 'volume': '36', 'author': 'Deen WM', 'year': '1977', 'journal-title': 'Federation Proc'} / Federation Proc by Deen WM (1977)
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10.1111/j.1748-1716.1994.tb09677.x
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{'key': 'B28', 'first-page': '243', 'volume': '41', 'author': 'Nielsen S.', 'year': '1994', 'journal-title': 'Dan Med Bull'} / Dan Med Bull by Nielsen S. (1994)
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10.1016/0304-4165(94)90064-7
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10.1046/j.1365-201X.1996.553306000.x

Dates

Type	When
Created	7 years, 8 months ago (Dec. 23, 2017, 9:16 a.m.)
Deposited	3 years ago (Aug. 10, 2022, 11:45 p.m.)
Indexed	1 year, 1 month ago (July 12, 2024, 9:10 a.m.)
Issued	24 years, 2 months ago (July 1, 2001)
Published	24 years, 2 months ago (July 1, 2001)
Published Print	24 years, 2 months ago (July 1, 2001)

Funders 0

None

BibTeX

@article{Ohlson_2001, title={Effects of filtration rate on the glomerular barrier and clearance of four differently shaped molecules}, volume={281}, ISSN={1522-1466}, url={http://dx.doi.org/10.1152/ajprenal.2001.281.1.f103}, DOI={10.1152/ajprenal.2001.281.1.f103}, number={1}, journal={American Journal of Physiology-Renal Physiology}, publisher={American Physiological Society}, author={Ohlson, Maria and Sörensson, Jenny and Lindström, Karin and Blom, Anna M. and Fries, Erik and Haraldsson, Börje}, year={2001}, month=jul, pages={F103–F113} }

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