DOI: 10.1152/ajplung.2001.281.6.l1453. Surfactant protein D enhances bacterial antigen presentation by bone marrow-derived dendritic cells

Surfactant protein D enhances bacterial antigen presentation by bone marrow-derived dendritic cells

10.1152/ajplung.2001.281.6.l1453

Crossref journal-article

American Physiological Society

American Journal of Physiology-Lung Cellular and Molecular Physiology (24)

Abstract

Surfactant protein (SP) D functions as a soluble pattern recognition molecule to mediate the clearance of pathogens by phagocytes in the innate immune response. We hypothesize that SP-D may also interact with dendritic cells, the most potent antigen presenting cell, to enhance uptake and presentation of bacterial antigens. Using mouse bone marrow-derived dendritic cells, we show that SP-D binds to immature dendritic cells in a dose-, carbohydrate-, and calcium-dependent manner, whereas SP-D binding to mature dendritic cells is reduced. SP-D also binds to Escherichia coli HB101 and enhances its association with dendritic cells. Additionally, SP-D enhances the antigen presentation of an ovalbumin fusion protein expressed in E. coli HB101 to ovalbumin-specific major histocompatibility complex class II T cell hybridomas. The enhancement of antigen presentation by SP-D is dose dependent and is not shared by other collectin-like proteins tested. These studies demonstrate that SP-D augments antigen presentation by dendritic cells and suggest that innate immune molecules such as SP-D may help initiate an adaptive immune response for the purpose of resolving an infection.

Bibliography

Brinker, K. G., Martin, E., Borron, P., Mostaghel, E., Doyle, C., Harding, C. V., & Wright, J. R. (2001). Surfactant protein D enhances bacterial antigen presentation by bone marrow-derived dendritic cells. American Journal of Physiology-Lung Cellular and Molecular Physiology, 281(6), L1453âL1463.

Authors 7

Karen G. Brinker (first)
Emily Martin (additional)
Paul Borron (additional)
Elahe Mostaghel (additional)
Carolyn Doyle (additional)
Clifford V. Harding (additional)
Jo Rae Wright (additional)

References 61 Referenced 79

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Dates

Type	When
Created	7 years, 8 months ago (Dec. 23, 2017, 4:56 p.m.)
Deposited	2 years ago (Aug. 30, 2023, 1:48 a.m.)
Indexed	3 weeks, 4 days ago (Aug. 5, 2025, 8:28 a.m.)
Issued	23 years, 8 months ago (Dec. 1, 2001)
Published	23 years, 8 months ago (Dec. 1, 2001)
Published Print	23 years, 8 months ago (Dec. 1, 2001)

Funders 0

None

BibTeX

@article{Brinker_2001, title={Surfactant protein D enhances bacterial antigen presentation by bone marrow-derived dendritic cells}, volume={281}, ISSN={1522-1504}, url={http://dx.doi.org/10.1152/ajplung.2001.281.6.l1453}, DOI={10.1152/ajplung.2001.281.6.l1453}, number={6}, journal={American Journal of Physiology-Lung Cellular and Molecular Physiology}, publisher={American Physiological Society}, author={Brinker, Karen G. and Martin, Emily and Borron, Paul and Mostaghel, Elahe and Doyle, Carolyn and Harding, Clifford V. and Wright, Jo Rae}, year={2001}, month=dec, pages={L1453–L1463} }

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