DOI: 10.1152/ajplung.2000.278.5.l946. Human SP-A protein variants derived from one or both genes stimulate TNF-α production in the THP-1 cell line

Human SP-A protein variants derived from one or both genes stimulate TNF-α production in the THP-1 cell line

10.1152/ajplung.2000.278.5.l946

Crossref journal-article

American Physiological Society

American Journal of Physiology-Lung Cellular and Molecular Physiology (24)

Abstract

In humans, two functional genes of surfactant protein (SP) A, SP-A1 and SP-A2, and several alleles of each functional gene have been characterized. SP-A is a multimeric molecule consisting of six trimers. Each trimer contains two SP-A1 molecules and one SP-A2 molecule. Until now, it has been unclear whether a single SP-Agene product is functional or whether there are functional differences either among alleles or between single-gene SP-A products and SP-A products derived from both genes. We tested the ability of in vitro expressed SP-A variants to stimulate tumor necrosis factor (TNF)-α production by THP-1 cells. We observed that 1) single-gene products and products derived from both genes stimulate TNF-α production, 2) there are differences among SP-A1 and SP-A2 alleles in their ability to stimulate TNF-α production, and 3) the increases in TNF-α production are lower after treatment with the SP-A1 alleles than after treatment with the SP-A2 alleles. Furthermore, coexpressed SP-As from SP-A1 and SP-A2 genes have a higher activity compared with SP-As from individual alleles or mixed SP-As from SP-A1and SP-A2 genes. These data suggest that the SP-A-induced increases in TNF-α levels differ among SP-A variants and appear to be affected by SP-A genotype and whether SP-A is derived from one or both genes.

Bibliography

Wang, G., Phelps, D. S., Umstead, T. M., & Floros, J. (2000). Human SP-A protein variants derived from one or both genes stimulate TNF-Î± production in the THP-1 cell line. American Journal of Physiology-Lung Cellular and Molecular Physiology, 278(5), L946âL954.

Authors 4

Guirong Wang (first)
David S. Phelps (additional)
Todd M. Umstead (additional)
Joanna Floros (additional)

References 40 Referenced 84

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Dates

Type	When
Created	7 years, 8 months ago (Dec. 23, 2017, 7:34 p.m.)
Deposited	3 years ago (Aug. 11, 2022, 12:18 a.m.)
Indexed	4 months, 1 week ago (April 15, 2025, 2:42 p.m.)
Issued	25 years, 3 months ago (May 1, 2000)
Published	25 years, 3 months ago (May 1, 2000)
Published Print	25 years, 3 months ago (May 1, 2000)

Funders 0

None

BibTeX

@article{Wang_2000, title={Human SP-A protein variants derived from one or both genes stimulate TNF-α production in the THP-1 cell line}, volume={278}, ISSN={1522-1504}, url={http://dx.doi.org/10.1152/ajplung.2000.278.5.l946}, DOI={10.1152/ajplung.2000.278.5.l946}, number={5}, journal={American Journal of Physiology-Lung Cellular and Molecular Physiology}, publisher={American Physiological Society}, author={Wang, Guirong and Phelps, David S. and Umstead, Todd M. and Floros, Joanna}, year={2000}, month=may, pages={L946–L954} }

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