DOI: 10.1152/ajpheart.01276.2005. Functional arrangement of rat diaphragmatic initial lymphatic network

Functional arrangement of rat diaphragmatic initial lymphatic network

10.1152/ajpheart.01276.2005

Crossref journal-article

American Physiological Society

American Journal of Physiology-Heart and Circulatory Physiology (24)

Abstract

Fluid and solute flux between the pleural and peritoneal cavities, although never documented under physiological conditions, might play a relevant role in pathological conditions associated with the development of ascitis and pleural effusion and/or in the processes of tumor dissemination. To verify whether a pleuroperitoneal flux might take place through the diaphragmatic lymphatic network, the transdiaphragmatic pressure gradient (ΔPTD) was measured in five spontaneously breathing anesthetized rats. ΔPTD was −1.93 cmH2O (SD 0.59) and −3.1 cmH2O (SD 0.82) at end expiration and at end inspiration, respectively, indicating the existence of a pressure gradient directed from the abdominal to the pleural cavity. Morphometrical analysis of the diaphragmatic lymphatic network was performed in the excised diaphragm of three additional rats euthanized with an anesthesia overdose. Optical and electron microscopy revealed that lymphatic submesothelial lacunae and lymphatic capillaries among the skeletal muscles fibers show the ultrastructural features of the so-called initial lymphatic vessels, namely, a discontinuous basal lamina and anchoring filaments linking the outer surface of the endothelial cells to connective tissue or to muscle fibers. Primary unidirectional valves in the wall of the initial lymphatics allow entrance of serosal fluid into the lymphatic network preventing fluid backflow, while unidirectional intraluminar valves in the transverse vessels convey lymph centripetally toward central collecting ducts. The complexity and anatomical arrangement of the two valves system suggests that, despite the existence of a favorable ΔPTD, in the physiological condition no fluid bulk flow takes place between the pleural and peritoneal cavity through the diaphragmatic lymphatic network.

Bibliography

Grimaldi, A., Moriondo, A., Sciacca, L., Guidali, M. L., Tettamanti, G., & Negrini, D. (2006). Functional arrangement of rat diaphragmatic initial lymphatic network. American Journal of Physiology-Heart and Circulatory Physiology, 291(2), H876âH885.

Authors 6

Annalisa Grimaldi (first)
Andrea Moriondo (additional)
Laura Sciacca (additional)
Maria Luisa Guidali (additional)
Gianluca Tettamanti (additional)
Daniela Negrini (additional)

References 27 Referenced 44

10.1152/physrev.1993.73.1.1
10.1006/mvre.1998.2101
10.1152/ajpheart.1989.257.6.H2059
Chow K, Szeto C, and Li P. Management options for hydrothorax complicating peritoneal dialysis. Senim Dial 16: 389–394, 2003. (10.1046/j.1525-139X.2003.16080.x)
10.1016/S1471-4906(01)01936-6
10.1083/jcb.36.1.129
McHale N. Role of lymph pump and its control. News Physiol Sci 10: 112–117, 1995. (10.1152/physiologyonline.1995.10.3.112)
10.1113/jphysiol.1976.sp011557
10.1115/1.1568128
10.1016/0034-5687(82)90030-5
10.1016/0034-5687(81)90068-2
10.1152/jappl.1989.66.4.1579
Negrini D and Del Fabbro M. Subatmospheric pressure in the rabbit pleural lymphatic network. J Physiol \?\520.3: 761–769, 1999. (10.1111/j.1469-7793.1999.00761.x)
10.1152/jappl.1992.72.3.1166
10.1152/jappl.1993.74.4.1779
Negrini D, Moriondo A, and Mukenge S. Intercostal lymphatic pressure in spontaneously breathing rats. FASEB J 18: 440.1, 2004.
10.1089/lrb.2004.2.69
10.1152/jappl.1991.70.4.1544
10.1152/jappl.1985.58.6.2062
10.1679/aohc.64.513
10.1111/1523-1747.ep12277336
10.1152/physrev.1990.70.4.987
10.1016/0026-2862(84)90032-3
10.1096/fj.01-0067com
10.1016/0730-725X(93)90228-6
Wang NS. The preformed stomata connecting the pleural cavity and the lymphatics in the parietal pleura. Am Rev Respir Dis 111: 12–20, 1975.
10.1152/ajpheart.1993.264.4.H1283

Dates

Type	When
Created	19 years, 6 months ago (Feb. 17, 2006, 8:34 p.m.)
Deposited	5 years, 11 months ago (Sept. 8, 2019, 4:19 p.m.)
Indexed	1 month ago (July 25, 2025, 6:11 a.m.)
Issued	19 years ago (Aug. 1, 2006)
Published	19 years ago (Aug. 1, 2006)
Published Print	19 years ago (Aug. 1, 2006)

Funders 0

None

BibTeX

@article{Grimaldi_2006, title={Functional arrangement of rat diaphragmatic initial lymphatic network}, volume={291}, ISSN={1522-1539}, url={http://dx.doi.org/10.1152/ajpheart.01276.2005}, DOI={10.1152/ajpheart.01276.2005}, number={2}, journal={American Journal of Physiology-Heart and Circulatory Physiology}, publisher={American Physiological Society}, author={Grimaldi, Annalisa and Moriondo, Andrea and Sciacca, Laura and Guidali, Maria Luisa and Tettamanti, Gianluca and Negrini, Daniela}, year={2006}, month=aug, pages={H876–H885} }

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