DOI: 10.1152/ajpheart.00515.2010. Cardiac myocyte remodeling mediated by N-cadherin-dependent mechanosensing [Penn]

Cardiac myocyte remodeling mediated by N-cadherin-dependent mechanosensing

10.1152/ajpheart.00515.2010

Crossref journal-article

American Physiological Society

American Journal of Physiology-Heart and Circulatory Physiology (24)

Abstract

Cell-to-cell adhesions are crucial in maintaining the structural and functional integrity of cardiac cells. Little is known about the mechanosensitivity and mechanotransduction of cell-to-cell interactions. Most studies of cardiac mechanotransduction and myofibrillogenesis have focused on cell-extracellular matrix (ECM)-specific interactions. This study assesses the direct role of intercellular adhesion, specifically that of N-cadherin-mediated mechanotransduction, on the morphology and internal organization of neonatal ventricular cardiac myocytes. The results show that cadherin-mediated cell attachments are capable of eliciting a cytoskeletal network response similar to that of integrin-mediated force response and transmission, affecting myofibrillar organization, myocyte shape, and cortical stiffness. Traction forces mediated by N-cadherin were shown to be comparable to those sustained by ECM. The directional changes in predicted traction forces as a function of imposed loads (gel stiffness) provide the added evidence that N-cadherin is a mechanoresponsive adhesion receptor. Strikingly, the mechanical sensitivity response (gain) in terms of the measured cell-spread area as a function of imposed load (adhesive substrate rigidity) was consistently higher for N-cadherin-coated surfaces compared with ECM protein-coated surfaces. In addition, the cytoskeletal architecture of myocytes on an N-cadherin adhesive microenvironment was characteristically different from that on an ECM environment, suggesting that the two mechanotransductive cell adhesion systems may play both independent and complementary roles in myocyte cytoskeletal spatial organization. These results indicate that cell-to-cell-mediated force perception and transmission are involved in the organization and development of cardiac structure and function.

Bibliography

Chopra, A., Tabdanov, E., Patel, H., Janmey, P. A., & Kresh, J. Y. (2011). Cardiac myocyte remodeling mediated by N-cadherin-dependent mechanosensing. American Journal of Physiology-Heart and Circulatory Physiology, 300(4), H1252âH1266.

Authors 5

Anant Chopra (first)
Erdem Tabdanov (additional)
Hersh Patel (additional)
Paul A. Janmey (additional)
J. Yasha Kresh (additional)

References 62 Referenced 113

10.1242/jcs.107.12.3655 / J Cell Sci by Aberle H (1994)
10.1016/S0955-0674(98)80031-8
10.1152/ajpheart.00427.2009
10.1073/pnas.0405747102
10.1007/978-1-59745-353-0_14
10.1152/ajpheart.01017.2005
10.1006/dbio.1996.0346
10.1083/jcb.110.4.1253
10.1016/j.bpj.2009.03.046
10.1002/ar.1092360311
10.1016/S0006-3495(99)77386-8
10.1016/j.devcel.2009.01.008
10.1016/S0006-3495(04)74140-5
10.1242/jcs.029678
10.1083/jcb.200405004
10.1016/j.cell.2006.06.044
10.1016/j.yjmcc.2009.08.003
10.1042/BC20060039
10.1242/jcs.00857
10.1074/jbc.M401705200
10.1155/2010/624719
10.1529/biophysj.105.073114
10.1242/dev.114.1.173 / Development by Goncharova EJ (1992)
10.1242/jcs.01496
10.1023/A:1012445316415
10.1016/j.jbiomech.2009.09.014
10.1529/biophysj.107.124545
10.1073/pnas.92.11.5067
10.1016/S0002-9440(10)64055-2
10.1161/01.RES.0000156274.72390.2c
10.1016/j.jtcvs.2006.02.018
10.1038/ncb1705
10.1016/j.bpj.2009.10.044
10.1242/jcs.113.12.2207 / J Cell Sci by Lambert M (2000)
10.1016/S0006-3495(00)76279-5
10.1242/jcs.00339 / J Cell Sci by Luo Y
10.1083/jcb.200612042
10.1161/01.RES.85.11.1046
10.1016/S1534-5807(04)00075-9
10.1016/j.ceb.2006.08.004
10.1242/jcs.108.6.2311 / J Cell Sci by Mooney DJ (1995)
10.1002/047001766X.ch13
10.1073/pnas.94.25.13661
10.1093/emboj/21.11.2537
10.1006/dbio.1996.8443
10.1002/cm.970280102
10.1634/stemcells.2008-0432
10.1152/jappl.2000.88.3.1127
10.1152/ajpheart.00749.2005
10.1126/science.1169414
10.1161/hh0402.105790
10.1016/j.actbio.2006.09.003
10.1242/jcs.046805
10.1529/biophysj.106.101386
10.1038/nrm2634
10.1016/0955-0674(95)80102-2
10.1073/pnas.78.12.7619
10.1016/0012-1606(64)90017-X
10.1073/pnas.0912739107
10.1089/ten.tea.2007.0388
10.1002/cm.20041
10.1088/0953-8984/22/19/194110

Dates

Type	When
Created	14 years, 7 months ago (Jan. 22, 2011, 12:33 a.m.)
Deposited	3 years, 9 months ago (Nov. 17, 2021, 4:31 p.m.)
Indexed	2 weeks, 2 days ago (Aug. 7, 2025, 4:58 a.m.)
Issued	14 years, 4 months ago (April 1, 2011)
Published	14 years, 4 months ago (April 1, 2011)
Published Print	14 years, 4 months ago (April 1, 2011)

Funders 0

None

BibTeX

@article{Chopra_2011, title={Cardiac myocyte remodeling mediated by N-cadherin-dependent mechanosensing}, volume={300}, ISSN={1522-1539}, url={http://dx.doi.org/10.1152/ajpheart.00515.2010}, DOI={10.1152/ajpheart.00515.2010}, number={4}, journal={American Journal of Physiology-Heart and Circulatory Physiology}, publisher={American Physiological Society}, author={Chopra, Anant and Tabdanov, Erdem and Patel, Hersh and Janmey, Paul A. and Kresh, J. Yasha}, year={2011}, month=apr, pages={H1252–H1266} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        8,
        7
      ]
    ],
    "date-time": "2025-08-07T08:58:42Z",
    "timestamp": 1754557122344
  },
  "reference-count": 62,
  "publisher": "American Physiological Society",
  "issue": "4",
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        2011,
        4
      ]
    ]
  },
  "abstract": "<jats:p>Cell-to-cell adhesions are crucial in maintaining the structural and functional integrity of cardiac cells. Little is known about the mechanosensitivity and mechanotransduction of cell-to-cell interactions. Most studies of cardiac mechanotransduction and myofibrillogenesis have focused on cell-extracellular matrix (ECM)-specific interactions. This study assesses the direct role of intercellular adhesion, specifically that of N-cadherin-mediated mechanotransduction, on the morphology and internal organization of neonatal ventricular cardiac myocytes. The results show that cadherin-mediated cell attachments are capable of eliciting a cytoskeletal network response similar to that of integrin-mediated force response and transmission, affecting myofibrillar organization, myocyte shape, and cortical stiffness. Traction forces mediated by N-cadherin were shown to be comparable to those sustained by ECM. The directional changes in predicted traction forces as a function of imposed loads (gel stiffness) provide the added evidence that N-cadherin is a mechanoresponsive adhesion receptor. Strikingly, the mechanical sensitivity response (gain) in terms of the measured cell-spread area as a function of imposed load (adhesive substrate rigidity) was consistently higher for N-cadherin-coated surfaces compared with ECM protein-coated surfaces. In addition, the cytoskeletal architecture of myocytes on an N-cadherin adhesive microenvironment was characteristically different from that on an ECM environment, suggesting that the two mechanotransductive cell adhesion systems may play both independent and complementary roles in myocyte cytoskeletal spatial organization. These results indicate that cell-to-cell-mediated force perception and transmission are involved in the organization and development of cardiac structure and function.</jats:p>",
  "DOI": "10.1152/ajpheart.00515.2010",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2011,
        1,
        22
      ]
    ],
    "date-time": "2011-01-22T05:33:42Z",
    "timestamp": 1295674422000
  },
  "page": "H1252-H1266",
  "source": "Crossref",
  "is-referenced-by-count": 113,
  "title": "Cardiac myocyte remodeling mediated by N-cadherin-dependent mechanosensing",
  "prefix": "10.1152",
  "volume": "300",
  "author": [
    {
      "given": "Anant",
      "family": "Chopra",
      "sequence": "first",
      "affiliation": [
        {
          "name": "Department of Biomedical Engineering, Drexel University,"
        },
        {
          "name": "Department of Cardiothoracic Surgery and Medicine, Drexel University College of Medicine, Philadelphia;"
        }
      ]
    },
    {
      "given": "Erdem",
      "family": "Tabdanov",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Cardiothoracic Surgery and Medicine, Drexel University College of Medicine, Philadelphia;"
        },
        {
          "name": "Department of Physiology, Institute for Medicine and Engineering, University of Pennsylvania, Philadelphia, Pennsylvania"
        }
      ]
    },
    {
      "given": "Hersh",
      "family": "Patel",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Cardiothoracic Surgery and Medicine, Drexel University College of Medicine, Philadelphia;"
        }
      ]
    },
    {
      "given": "Paul A.",
      "family": "Janmey",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Physiology, Institute for Medicine and Engineering, University of Pennsylvania, Philadelphia, Pennsylvania"
        }
      ]
    },
    {
      "given": "J. Yasha",
      "family": "Kresh",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Biomedical Engineering, Drexel University,"
        },
        {
          "name": "Department of Cardiothoracic Surgery and Medicine, Drexel University College of Medicine, Philadelphia;"
        },
        {
          "name": "Department of Physiology, Institute for Medicine and Engineering, University of Pennsylvania, Philadelphia, Pennsylvania"
        }
      ]
    }
  ],
  "member": "24",
  "reference": [
    {
      "key": "B1",
      "doi-asserted-by": "crossref",
      "first-page": "3655",
      "DOI": "10.1242/jcs.107.12.3655",
      "volume": "107",
      "author": "Aberle H",
      "year": "1994",
      "journal-title": "J Cell Sci"
    },
    {
      "key": "B2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0955-0674(98)80031-8"
    },
    {
      "key": "B3",
      "doi-asserted-by": "publisher",
      "DOI": "10.1152/ajpheart.00427.2009"
    },
    {
      "key": "B4",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.0405747102"
    },
    {
      "key": "B5",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/978-1-59745-353-0_14"
    },
    {
      "key": "B6",
      "doi-asserted-by": "publisher",
      "DOI": "10.1152/ajpheart.01017.2005"
    },
    {
      "key": "B7",
      "doi-asserted-by": "publisher",
      "DOI": "10.1006/dbio.1996.0346"
    },
    {
      "key": "B8",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.110.4.1253"
    },
    {
      "key": "B9",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.bpj.2009.03.046"
    },
    {
      "key": "B10",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/ar.1092360311"
    },
    {
      "key": "B11",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0006-3495(99)77386-8"
    },
    {
      "key": "B12",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.devcel.2009.01.008"
    },
    {
      "key": "B13",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0006-3495(04)74140-5"
    },
    {
      "key": "B14",
      "doi-asserted-by": "publisher",
      "DOI": "10.1242/jcs.029678"
    },
    {
      "key": "B15",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.200405004"
    },
    {
      "key": "B16",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.cell.2006.06.044"
    },
    {
      "key": "B17",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.yjmcc.2009.08.003"
    },
    {
      "key": "B18",
      "doi-asserted-by": "publisher",
      "DOI": "10.1042/BC20060039"
    },
    {
      "key": "B19",
      "doi-asserted-by": "publisher",
      "DOI": "10.1242/jcs.00857"
    },
    {
      "key": "B20",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.M401705200"
    },
    {
      "key": "B21",
      "doi-asserted-by": "publisher",
      "DOI": "10.1155/2010/624719"
    },
    {
      "key": "B22",
      "doi-asserted-by": "publisher",
      "DOI": "10.1529/biophysj.105.073114"
    },
    {
      "key": "B23",
      "doi-asserted-by": "crossref",
      "first-page": "173",
      "DOI": "10.1242/dev.114.1.173",
      "volume": "114",
      "author": "Goncharova EJ",
      "year": "1992",
      "journal-title": "Development"
    },
    {
      "key": "B24",
      "doi-asserted-by": "publisher",
      "DOI": "10.1242/jcs.01496"
    },
    {
      "key": "B25",
      "doi-asserted-by": "publisher",
      "DOI": "10.1023/A:1012445316415"
    },
    {
      "key": "B26",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.jbiomech.2009.09.014"
    },
    {
      "key": "B27",
      "doi-asserted-by": "publisher",
      "DOI": "10.1529/biophysj.107.124545"
    },
    {
      "key": "B28",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.92.11.5067"
    },
    {
      "key": "B29",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0002-9440(10)64055-2"
    },
    {
      "key": "B30",
      "doi-asserted-by": "publisher",
      "DOI": "10.1161/01.RES.0000156274.72390.2c"
    },
    {
      "key": "B31",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.jtcvs.2006.02.018"
    },
    {
      "key": "B32",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/ncb1705"
    },
    {
      "key": "B33",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.bpj.2009.10.044"
    },
    {
      "key": "B34",
      "doi-asserted-by": "crossref",
      "first-page": "2207",
      "DOI": "10.1242/jcs.113.12.2207",
      "volume": "113",
      "author": "Lambert M",
      "year": "2000",
      "journal-title": "J Cell Sci"
    },
    {
      "key": "B35",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0006-3495(00)76279-5"
    },
    {
      "key": "B36",
      "doi-asserted-by": "crossref",
      "first-page": "1471",
      "DOI": "10.1242/jcs.00339",
      "volume": "116",
      "author": "Luo Y",
      "journal-title": "J Cell Sci"
    },
    {
      "key": "B37",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.200612042"
    },
    {
      "key": "B38",
      "doi-asserted-by": "publisher",
      "DOI": "10.1161/01.RES.85.11.1046"
    },
    {
      "key": "B39",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S1534-5807(04)00075-9"
    },
    {
      "key": "B40",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.ceb.2006.08.004"
    },
    {
      "key": "B41",
      "doi-asserted-by": "crossref",
      "first-page": "2311",
      "DOI": "10.1242/jcs.108.6.2311",
      "volume": "108",
      "author": "Mooney DJ",
      "year": "1995",
      "journal-title": "J Cell Sci"
    },
    {
      "key": "B42",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/047001766X.ch13"
    },
    {
      "key": "B43",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.94.25.13661"
    },
    {
      "key": "B44",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/emboj/21.11.2537"
    },
    {
      "key": "B45",
      "doi-asserted-by": "publisher",
      "DOI": "10.1006/dbio.1996.8443"
    },
    {
      "key": "B46",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/cm.970280102"
    },
    {
      "key": "B47",
      "doi-asserted-by": "publisher",
      "DOI": "10.1634/stemcells.2008-0432"
    },
    {
      "key": "B48",
      "doi-asserted-by": "publisher",
      "DOI": "10.1152/jappl.2000.88.3.1127"
    },
    {
      "key": "B49",
      "doi-asserted-by": "publisher",
      "DOI": "10.1152/ajpheart.00749.2005"
    },
    {
      "key": "B50",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.1169414"
    },
    {
      "key": "B51",
      "doi-asserted-by": "publisher",
      "DOI": "10.1161/hh0402.105790"
    },
    {
      "key": "B52",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.actbio.2006.09.003"
    },
    {
      "key": "B53",
      "doi-asserted-by": "publisher",
      "DOI": "10.1242/jcs.046805"
    },
    {
      "key": "B54",
      "doi-asserted-by": "publisher",
      "DOI": "10.1529/biophysj.106.101386"
    },
    {
      "key": "B55",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/nrm2634"
    },
    {
      "key": "B56",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0955-0674(95)80102-2"
    },
    {
      "key": "B57",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.78.12.7619"
    },
    {
      "key": "B58",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0012-1606(64)90017-X"
    },
    {
      "key": "B59",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.0912739107"
    },
    {
      "key": "B60",
      "doi-asserted-by": "publisher",
      "DOI": "10.1089/ten.tea.2007.0388"
    },
    {
      "key": "B61",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/cm.20041"
    },
    {
      "key": "B62",
      "doi-asserted-by": "publisher",
      "DOI": "10.1088/0953-8984/22/19/194110"
    }
  ],
  "container-title": "American Journal of Physiology-Heart and Circulatory Physiology",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://www.physiology.org/doi/pdf/10.1152/ajpheart.00515.2010",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2021,
        11,
        17
      ]
    ],
    "date-time": "2021-11-17T21:31:13Z",
    "timestamp": 1637184673000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://www.physiology.org/doi/10.1152/ajpheart.00515.2010"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        2011,
        4
      ]
    ]
  },
  "references-count": 62,
  "journal-issue": {
    "issue": "4",
    "published-print": {
      "date-parts": [
        [
          2011,
          4
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1152/ajpheart.00515.2010"
  ],
  "URL": "http://dx.doi.org/10.1152/ajpheart.00515.2010",
  "relation": {},
  "ISSN": [
    "0363-6135",
    "1522-1539"
  ],
  "subject": [],
  "container-title-short": "American Journal of Physiology-Heart and Circulatory Physiology",
  "published": {
    "date-parts": [
      [
        2011,
        4
      ]
    ]
  }
}