DOI: 10.1152/ajpcell.2001.281.6.c1917. Gap junctions and fluid flow response in MC3T3-E1 cells

Gap junctions and fluid flow response in MC3T3-E1 cells

10.1152/ajpcell.2001.281.6.c1917

Crossref journal-article

American Physiological Society

American Journal of Physiology-Cell Physiology (24)

Abstract

In the current study, we examined the role of gap junctions in oscillatory fluid flow-induced changes in intracellular Ca2+concentration and prostaglandin release in osteoblastic cells. This work was completed in MC3T3-E1 cells with intact gap junctional communication as well as in MC3T3-E1 cells rendered communication deficient through expression of a dominant-negative connexin. Our results demonstrate that MC3T3-E1 cells with intact gap junctions respond to oscillatory fluid flow with significant increases in prostaglandin E2 (PGE2) release, whereas cells with diminished gap junctional communication do not. Furthermore, we found that cytosolic Ca2+ (Ca[Formula: see text]) response was unaltered by the disruption in gap junctional communication and was not significantly different among the cell lines. Thus our results suggest that gap junctions contribute to the PGE2 but not to the Ca[Formula: see text] response to oscillatory fluid flow. These findings implicate gap junctional intercellular communication (GJIC) in bone cell ensemble responsiveness to oscillatory fluid flow and suggest that gap junctions and GJIC play a pivotal role in mechanotransduction mechanisms in bone.

Bibliography

Saunders, M. M., You, J., Trosko, J. E., Yamasaki, H., Li, Z., Donahue, H. J., & Jacobs, C. R. (2001). Gap junctions and fluid flow response in MC3T3-E1 cells. American Journal of Physiology-Cell Physiology, 281(6), C1917âC1925.

Authors 7

M. M. Saunders (first)
J. You (additional)
J. E. Trosko (additional)
H. Yamasaki (additional)
Z. Li (additional)
H. J. Donahue (additional)
C. R. Jacobs (additional)

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Dates

Type	When
Created	7 years, 8 months ago (Dec. 24, 2017, 3:37 p.m.)
Deposited	1 year, 2 months ago (June 17, 2024, 3:19 p.m.)
Indexed	1 year, 2 months ago (June 17, 2024, 8:06 p.m.)
Issued	23 years, 8 months ago (Dec. 1, 2001)
Published	23 years, 8 months ago (Dec. 1, 2001)
Published Print	23 years, 8 months ago (Dec. 1, 2001)

Funders 0

None

BibTeX

@article{Saunders_2001, title={Gap junctions and fluid flow response in MC3T3-E1 cells}, volume={281}, ISSN={1522-1563}, url={http://dx.doi.org/10.1152/ajpcell.2001.281.6.c1917}, DOI={10.1152/ajpcell.2001.281.6.c1917}, number={6}, journal={American Journal of Physiology-Cell Physiology}, publisher={American Physiological Society}, author={Saunders, M. M. and You, J. and Trosko, J. E. and Yamasaki, H. and Li, Z. and Donahue, H. J. and Jacobs, C. R.}, year={2001}, month=dec, pages={C1917–C1925} }

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  "abstract": "<jats:p> In the current study, we examined the role of gap junctions in oscillatory fluid flow-induced changes in intracellular Ca<jats:sup>2+</jats:sup>concentration and prostaglandin release in osteoblastic cells. This work was completed in MC3T3-E1 cells with intact gap junctional communication as well as in MC3T3-E1 cells rendered communication deficient through expression of a dominant-negative connexin. Our results demonstrate that MC3T3-E1 cells with intact gap junctions respond to oscillatory fluid flow with significant increases in prostaglandin E<jats:sub>2</jats:sub> (PGE<jats:sub>2</jats:sub>) release, whereas cells with diminished gap junctional communication do not. Furthermore, we found that cytosolic Ca<jats:sup>2+</jats:sup> (Ca[Formula: see text]) response was unaltered by the disruption in gap junctional communication and was not significantly different among the cell lines. Thus our results suggest that gap junctions contribute to the PGE<jats:sub>2</jats:sub> but not to the Ca[Formula: see text] response to oscillatory fluid flow. These findings implicate gap junctional intercellular communication (GJIC) in bone cell ensemble responsiveness to oscillatory fluid flow and suggest that gap junctions and GJIC play a pivotal role in mechanotransduction mechanisms in bone. </jats:p>",
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