DOI: 10.1152/ajpcell.1999.277.4.c616. Distinct pharmacological properties of ET-1 and ET-3 on astroglial gap junctions and Ca<sup>2+</sup>signaling

Distinct pharmacological properties of ET-1 and ET-3 on astroglial gap junctions and Ca2+signaling

10.1152/ajpcell.1999.277.4.c616

Crossref journal-article

American Physiological Society

American Journal of Physiology-Cell Physiology (24)

Abstract

Astrocytes represent a major target for endothelins (ETs), a family of peptides that have potent and multiple effects on signal transduction pathways and can be released by several cell types in the brain. In the present study we have investigated the involvement of different ET receptor subtypes on intercellular dye diffusion, intracellular Ca2+homeostasis, and intercellular Ca2+signaling in cultured rat astrocytes from hippocampus and striatum. Depending on the ET concentration and the receptor involved, ET-1- and ET-3-induced intracellular Ca2+increases with different response patterns. Both ET-1 and ET-3 are powerful inhibitors of gap junctional permeability and intercellular Ca2+signaling. The nonselective ET receptor agonist sarafotoxin S6b and the ETBreceptor-selective agonist IRL 1620 mimicked these inhibitions. The ET-3 effects were only marginally affected by an ETAreceptor antagonist but completely blocked by an ETBreceptor antagonist. However, the ET-1-induced inhibition of gap junctional dye transfer and intercellular Ca2+signaling was only marginally blocked by ETAor ETBreceptor-selective antagonists but fully prevented when these antagonists were applied together. The ET-induced inhibition of gap junction permeability and intercellular Ca2+signaling indicates that important changes in the function of astroglial communication might occur when the level of ETs in the brain is increased.

Bibliography

Blomstrand, F., Giaume, C., Hansson, E., & RÃ¶nnbÃ¤ck, L. (1999). Distinct pharmacological properties of ET-1 and ET-3 on astroglial gap junctions and Ca2+signaling. American Journal of Physiology-Cell Physiology, 277(4), C616âC627.

Authors 4

Fredrik Blomstrand (first)
Christian Giaume (additional)
Elisabeth Hansson (additional)
Lars Rönnbäck (additional)

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Dates

Type	When
Created	7 years, 8 months ago (Dec. 24, 2017, 5:40 p.m.)
Deposited	2 months, 1 week ago (June 29, 2025, 1:24 a.m.)
Indexed	2 months, 1 week ago (June 29, 2025, 1:40 a.m.)
Issued	25 years, 11 months ago (Oct. 1, 1999)
Published	25 years, 11 months ago (Oct. 1, 1999)
Published Print	25 years, 11 months ago (Oct. 1, 1999)

Funders 0

None

BibTeX

@article{Blomstrand_1999, title={Distinct pharmacological properties of ET-1 and ET-3 on astroglial gap junctions and Ca2+signaling}, volume={277}, ISSN={1522-1563}, url={http://dx.doi.org/10.1152/ajpcell.1999.277.4.c616}, DOI={10.1152/ajpcell.1999.277.4.c616}, number={4}, journal={American Journal of Physiology-Cell Physiology}, publisher={American Physiological Society}, author={Blomstrand, Fredrik and Giaume, Christian and Hansson, Elisabeth and Rönnbäck, Lars}, year={1999}, month=oct, pages={C616–C627} }

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