DOI: 10.1152/ajpcell.1999.276.3.c621. Expression and localization of aquaporins in rat gastrointestinal tract

Expression and localization of aquaporins in rat gastrointestinal tract

10.1152/ajpcell.1999.276.3.c621

Crossref journal-article

American Physiological Society

American Journal of Physiology-Cell Physiology (24)

Abstract

A family of water-selective channels, aquaporins (AQP), has been demonstrated in various organs and tissues. However, the localization and expression of the AQP family members in the gastrointestinal tract have not been entirely elucidated. This study aimed to demonstrate the expression and distribution of several types of the AQP family and to speculate on their role in water transport in the rat gastrointestinal tract. By RNase protection assay, expression of AQP1–5 and AQP8 was examined in various portions through the gastrointestinal tract. AQP1 and AQP3 mRNAs were diffusely expressed from esophagus to colon, and their expression was relatively intense in the small intestine and colon. In contrast, AQP4 mRNA was selectively expressed in the stomach and small intestine and AQP8 mRNA in the jejunum and colon. Immunohistochemistry and in situ hybridization demonstrated cellular localization of these AQP in these portions. AQP1 was localized on endothelial cells of lymphatic vessels in the submucosa and lamina propria throughout the gastrointestinal tract. AQP3 was detected on the circumferential plasma membranes of stratified squamous epithelial cells in the esophagus and basolateral membranes of cardiac gland epithelia in the lower stomach and of surface columnar epithelia in the colon. However, AQP3 was not apparently detected in the small intestine. AQP4 was present on the basolateral membrane of the parietal cells in the lower stomach and selectively in the basolateral membranes of deep intestinal gland cells in the small intestine. AQP8 mRNA expression was demonstrated in the absorptive columnar epithelial cells of the jejunum and colon by in situ hybridization. These findings may indicate that water crosses the epithelial layer through these water channels, suggesting a possible role of the transcellular route for water intake or outlet in the gastrointestinal tract.

Bibliography

Koyama, Y., Yamamoto, T., Tani, T., Nihei, K., Kondo, D., Funaki, H., Yaoita, E., Kawasaki, K., Sato, N., Hatakeyama, K., & Kihara, I. (1999). Expression and localization of aquaporins in rat gastrointestinal tract. American Journal of Physiology-Cell Physiology, 276(3), C621âC627.

Authors 11

Yu Koyama (first)
Tadashi Yamamoto (additional)
Tatsuo Tani (additional)
Kouei Nihei (additional)
Daisuke Kondo (additional)
Haruko Funaki (additional)
Eishin Yaoita (additional)
Katsutoshi Kawasaki (additional)
Nobuaki Sato (additional)
Katsuyoshi Hatakeyama (additional)
Itaru Kihara (additional)

References 34 Referenced 153

10.1073/pnas.91.23.10997
10.1073/pnas.92.10.4328
10.1242/jcs.108.9.2993
10.1152/ajpcell.1998.275.4.C1151
10.1038/361549a0
10.1016/S0021-9258(17)37486-0
10.1074/jbc.272.33.20782
10.1006/bbrc.1998.8252
10.1006/bbrc.1997.7219
10.1073/pnas.91.14.6269
10.1073/pnas.91.26.13052
10.1146/annurev.ph.58.030196.003155
10.1074/jbc.272.48.30329
10.1006/bbrc.1997.7769
10.1016/0016-5085(90)90606-2
10.1016/S0021-9258(17)31880-X
10.1172/JCI231
10.1006/geno.1996.0396
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{'key': 'B20', 'first-page': '902', 'volume': '12', 'author': 'Masters D. B.', 'year': '1992', 'journal-title': 'Biotechniques'} / Biotechniques by Masters D. B. (1992)
10.1016/0014-5793(96)00092-0
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{'issue': '4', 'key': 'B26', 'first-page': 'G275', 'volume': '241', 'author': 'Powell D. W.', 'year': '1981', 'journal-title': 'Am. J. Physiol.'} / Am. J. Physiol. by Powell D. W. (1981)
Powell D. W.Intestinal water and electrolyte transport.Physiology of the Gastrointestinal Tract2nd Ed., Johnson L. R.2198712671305RavenNew York
10.1126/science.256.5055.385
10.1126/science.7521540
10.1074/jbc.270.4.1908
10.1007/BF01870707
10.1007/BF01871223
10.1152/ajprenal.1997.272.2.F198
10.1152/ajpcell.1995.268.6.C1546

Dates

Type	When
Created	7 years, 8 months ago (Dec. 24, 2017, 5:22 p.m.)
Deposited	1 year, 2 months ago (June 17, 2024, 3:23 p.m.)
Indexed	5 days, 19 hours ago (Aug. 23, 2025, 1:15 a.m.)
Issued	26 years, 5 months ago (March 1, 1999)
Published	26 years, 5 months ago (March 1, 1999)
Published Print	26 years, 5 months ago (March 1, 1999)

Funders 0

None

BibTeX

@article{Koyama_1999, title={Expression and localization of aquaporins in rat gastrointestinal tract}, volume={276}, ISSN={1522-1563}, url={http://dx.doi.org/10.1152/ajpcell.1999.276.3.c621}, DOI={10.1152/ajpcell.1999.276.3.c621}, number={3}, journal={American Journal of Physiology-Cell Physiology}, publisher={American Physiological Society}, author={Koyama, Yu and Yamamoto, Tadashi and Tani, Tatsuo and Nihei, Kouei and Kondo, Daisuke and Funaki, Haruko and Yaoita, Eishin and Kawasaki, Katsutoshi and Sato, Nobuaki and Hatakeyama, Katsuyoshi and Kihara, Itaru}, year={1999}, month=mar, pages={C621–C627} }

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