DOI: 10.1152/ajpcell.1998.274.3.c603. Activity and protein localization of multiple glutamate transporters in gestation<i>day 14</i>vs.<i>day 20</i>rat placenta

Activity and protein localization of multiple glutamate transporters in gestationday 14vs.day 20rat placenta

10.1152/ajpcell.1998.274.3.c603

Crossref journal-article

American Physiological Society

American Journal of Physiology-Cell Physiology (24)

Abstract

Concentrative absorption of glutamate by the developing placenta is critical for proper fetal development. The expression of GLAST1, GLT1, EAAC1, and EAAT4, known to be capable ofd-aspartate-inhibitable and Na+-coupled glutamate transport (system [Formula: see text]), was evaluated in day 14 vs. day 20 rat chorioallantoic placenta. Steady-state mRNA levels were greater at day 20 for all transporters. Immunohistochemistry determined that the expression of GLAST1, GLT1, and EAAC1 was greater throughout the day 20 placenta and was asymmetric with respect to cellular localization. EAAT4 protein was not detected. System[Formula: see text] activity was responsible for most of the Na+-dependent glutamate uptake and was greater in day 20 than in day 14apical and basal membrane subdomains of the labyrinth syncytiotrophoblast. Greater quantities of EAAC1 and GLAST1 protein were identified on day 20, and quantities were greater in basal than in apical membranes. GLT1 expression, unchanged in apical membranes, was decreased in basal membranes. These data correlate transporter mRNA and protein content with transport activity and demonstrate an increasing capacity for glutamate absorption by the developing placenta.

Bibliography

Matthews, J. C., Beveridge, M. J., Malandro, M. S., Rothstein, J. D., Campbell-Thompson, M., Verlander, J. W., Kilberg, M. S., & Novak, D. A. (1998). Activity and protein localization of multiple glutamate transporters in gestationday 14vs.day 20rat placenta. American Journal of Physiology-Cell Physiology, 274(3), C603âC614.

Authors 8

James C. Matthews (first)
Mark J. Beveridge (additional)
Marc S. Malandro (additional)
Jeffrey D. Rothstein (additional)
Martha Campbell-Thompson (additional)
Jill W. Verlander (additional)
Michael S. Kilberg (additional)
Donald A. Novak (additional)

References 38 Referenced 62

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10.1016/S0021-9258(19)69127-1
10.1046/j.1471-4159.1995.65062800.x
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{'key': 'B15', 'first-page': '1554', 'volume': '77', 'author': 'Jansson T.', 'year': '1993', 'journal-title': 'J. Clin. Endocrinol. Metab.'} / J. Clin. Endocrinol. Metab. by Jansson T. (1993)
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10.1152/ajpcell.1994.267.3.C804
10.1152/ajpcell.1996.271.1.C295
10.1006/abio.1997.2432
10.1007/BF00317652
10.1152/ajpcell.1989.257.5.C1005
{'issue': '36', 'key': 'B25', 'first-page': 'R89', 'volume': '267', 'author': 'Moores R. R.', 'year': '1994', 'journal-title': 'Am. J. Physiol.'} / Am. J. Physiol. by Moores R. R. (1994)
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10.1152/ajpcell.1996.270.1.C67

Dates

Type	When
Created	7 years, 8 months ago (Dec. 24, 2017, 11:46 a.m.)
Deposited	2 months ago (June 29, 2025, 1:14 a.m.)
Indexed	2 months ago (June 29, 2025, 1:40 a.m.)
Issued	27 years, 6 months ago (March 1, 1998)
Published	27 years, 6 months ago (March 1, 1998)
Published Print	27 years, 6 months ago (March 1, 1998)

Funders 0

None

BibTeX

@article{Matthews_1998, title={Activity and protein localization of multiple glutamate transporters in gestationday 14vs.day 20rat placenta}, volume={274}, ISSN={1522-1563}, url={http://dx.doi.org/10.1152/ajpcell.1998.274.3.c603}, DOI={10.1152/ajpcell.1998.274.3.c603}, number={3}, journal={American Journal of Physiology-Cell Physiology}, publisher={American Physiological Society}, author={Matthews, James C. and Beveridge, Mark J. and Malandro, Marc S. and Rothstein, Jeffrey D. and Campbell-Thompson, Martha and Verlander, Jill W. and Kilberg, Michael S. and Novak, Donald A.}, year={1998}, month=mar, pages={C603–C614} }

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