DOI: 10.1152/ajpcell.1982.242.5.c382. Glucose-induced proton uptake in secretory granules of beta-cells in monolayer culture

Glucose-induced proton uptake in secretory granules of beta-cells in monolayer culture

10.1152/ajpcell.1982.242.5.c382

Crossref journal-article

American Physiological Society

American Journal of Physiology-Cell Physiology (24)

Abstract

The weak base acridine orange (AO) has been shown to be accumulated by the insulin-containing secretory granules of cultured beta-cells in response to high glucose. Various lines of evidence indicate that this accumulation is due to a pH gradient. Thus ionophores such as monensin and nigericin abolish the glucose-induced accumulation, and a high concentration of the weak base, benzylamine, results in swelling of the granules. In the absence of glucose, ATP addition to digitonin-permeabilized cells also results in dye uptake. These data also suggest that a primary or secondary active accumulation mechanism for hydrogen ions exists across the granule membrane.

Bibliography

Pace, C. S., & Sachs, G. (1982). Glucose-induced proton uptake in secretory granules of beta-cells in monolayer culture. American Journal of Physiology-Cell Physiology, 242(5), C382âC387.

Authors 2

C. S. Pace (first)
G. Sachs (additional)

References 0 Referenced 28

None

Dates

Type	When
Created	7 years, 8 months ago (Dec. 24, 2017, 6:07 a.m.)
Deposited	1 year, 2 months ago (June 17, 2024, 3:56 p.m.)
Indexed	1 year, 2 months ago (June 17, 2024, 8:07 p.m.)
Issued	43 years, 3 months ago (May 1, 1982)
Published	43 years, 3 months ago (May 1, 1982)
Published Print	43 years, 3 months ago (May 1, 1982)

Funders 0

None

BibTeX

@article{Pace_1982, title={Glucose-induced proton uptake in secretory granules of beta-cells in monolayer culture}, volume={242}, ISSN={1522-1563}, url={http://dx.doi.org/10.1152/ajpcell.1982.242.5.c382}, DOI={10.1152/ajpcell.1982.242.5.c382}, number={5}, journal={American Journal of Physiology-Cell Physiology}, publisher={American Physiological Society}, author={Pace, C. S. and Sachs, G.}, year={1982}, month=may, pages={C382–C387} }

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