DOI: 10.1152/ajpcell.00425.2001. Stiffness changes in cultured airway smooth muscle cells

Stiffness changes in cultured airway smooth muscle cells

10.1152/ajpcell.00425.2001

Crossref journal-article

American Physiological Society

American Journal of Physiology-Cell Physiology (24)

Abstract

Airway smooth muscle (ASM) cells in culture stiffen when exposed to contractile agonists. Such cell stiffening may reflect activation of the contractile apparatus as well as polymerization of cytoskeletal biopolymers. Here we have assessed the relative contribution of these mechanisms in cultured ASM cells stimulated with serotonin (5-hydroxytryptamine; 5-HT) in the presence or absence of drugs that inhibit either myosin-based contraction or polymerization of filamentous (F) actin. Magnetic twisting cytometry was used to measure cell stiffness, and associated changes in structural organization of actin cytoskeleton were evaluated by confocal microscopy. We found that 5-HT increased cell stiffness in a dose-dependent fashion and also elicited rapid formation of F-actin as marked by increased intensity of FITC-phalloidin staining in these cells. A calmodulin antagonist (W-7), a myosin light chain kinase inhibitor (ML-7) and a myosin ATPase inhibitor (BDM) each ablated the stiffening response but not the F-actin polymerization induced by 5-HT. Agents that inhibited the formation of F-actin (cytochalasin D, latrunculin A, C3 exoenzyme, and Y-27632) attenuated both baseline stiffness and the extent of cell stiffening in response to 5-HT. Together, these data suggest that agonist-evoked stiffening of cultured ASM cells requires actin polymerization as well as myosin activation and that neither actin polymerization nor myosin activation by itself is sufficient to account for the cell stiffening response.

Bibliography

An, S. S., Laudadio, R. E., Lai, J., Rogers, R. A., & Fredberg, J. J. (2002). Stiffness changes in cultured airway smooth muscle cells. American Journal of Physiology-Cell Physiology, 283(3), C792âC801.

Authors 5

Steven S. An (first)
Rachel E. Laudadio (additional)
Jean Lai (additional)
Rick A. Rogers (additional)
Jeffrey J. Fredberg (additional)

References 46 Referenced 138

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Dates

Type	When
Created	12 years, 3 months ago (May 13, 2013, 8:10 p.m.)
Deposited	1 year, 2 months ago (June 17, 2024, 3:13 p.m.)
Indexed	2 months ago (July 2, 2025, 2:35 p.m.)
Issued	23 years ago (Sept. 1, 2002)
Published	23 years ago (Sept. 1, 2002)
Published Print	23 years ago (Sept. 1, 2002)

Funders 0

None

BibTeX

@article{An_2002, title={Stiffness changes in cultured airway smooth muscle cells}, volume={283}, ISSN={1522-1563}, url={http://dx.doi.org/10.1152/ajpcell.00425.2001}, DOI={10.1152/ajpcell.00425.2001}, number={3}, journal={American Journal of Physiology-Cell Physiology}, publisher={American Physiological Society}, author={An, Steven S. and Laudadio, Rachel E. and Lai, Jean and Rogers, Rick A. and Fredberg, Jeffrey J.}, year={2002}, month=sep, pages={C792–C801} }

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