DOI: 10.1152/ajpcell.00420.2001. Human Na<sup>+</sup>/H<sup>+</sup> exchanger isoform 6 is found in recycling endosomes of cells, not in mitochondria

Human Na+/H+ exchanger isoform 6 is found in recycling endosomes of cells, not in mitochondria

10.1152/ajpcell.00420.2001

Crossref journal-article

American Physiological Society

American Journal of Physiology-Cell Physiology (24)

Abstract

Since the discovery of the first intracellular Na+/H+exchanger in yeast, Nhx1, multiple homologs have been cloned and characterized in plants. Together, studies in these organisms demonstrate that Nhx1 is located in the prevacuolar/vacuolar compartment of cells where it sequesters Na+ into the vacuole, regulates intravesicular pH, and contributes to vacuolar biogenesis. In contrast, the human homolog of Nhx1, Na+/H+ exchanger isoform 6 (NHE6), has been reported to localize to mitochondria when transiently expressed as a fusion with green fluorescent protein. This result warrants reevaluation because it conflicts with predictions from phylogenetic analyses. Here we demonstrate that when epitope-tagged NHE6 is transiently expressed in cultured mammalian cells, it does not colocalize with mitochondrial markers. It also does not colocalize with markers of the lysosome, late endosome, trans-Golgi network, or Golgi cisternae. Rather, NHE6 is distributed in recycling compartments and transiently appears on the plasma membrane. These results suggest that, like its homologs in yeast and plants, NHE6 is an endosomal Na+/H+ exchanger that may regulate intravesicular pH and volume and contribute to lysosomal biogenesis.

Bibliography

Brett, C. L., Wei, Y., Donowitz, M., & Rao, R. (2002). Human Na+/H+ exchanger isoform 6 is found in recycling endosomes of cells, not in mitochondria. American Journal of Physiology-Cell Physiology, 282(5), C1031âC1041.

Authors 4

Christopher L. Brett (first)
Ying Wei (additional)
Mark Donowitz (additional)
Rajini Rao (additional)

References 37 Referenced 150

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Dates

Type	When
Created	12 years, 3 months ago (May 13, 2013, 8:08 p.m.)
Deposited	1 year, 2 months ago (June 17, 2024, 3:09 p.m.)
Indexed	2 months ago (July 2, 2025, 2:47 p.m.)
Issued	23 years, 4 months ago (May 1, 2002)
Published	23 years, 4 months ago (May 1, 2002)
Published Print	23 years, 4 months ago (May 1, 2002)

Funders 0

None

BibTeX

@article{Brett_2002, title={Human Na+/H+ exchanger isoform 6 is found in recycling endosomes of cells, not in mitochondria}, volume={282}, ISSN={1522-1563}, url={http://dx.doi.org/10.1152/ajpcell.00420.2001}, DOI={10.1152/ajpcell.00420.2001}, number={5}, journal={American Journal of Physiology-Cell Physiology}, publisher={American Physiological Society}, author={Brett, Christopher L. and Wei, Ying and Donowitz, Mark and Rao, Rajini}, year={2002}, month=may, pages={C1031–C1041} }

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