DOI: 10.1146/annurev.physiol.59.1.621. INDUCED MEMBRANE HYPO/HYPER-MECHANOSENSITIVITY:A Limitation of Patch-Clamp Recording

INDUCED MEMBRANE HYPO/HYPER-MECHANOSENSITIVITY:A Limitation of Patch-Clamp Recording

10.1146/annurev.physiol.59.1.621

Crossref journal-article

Annual Reviews

Annual Review of Physiology (22)

Abstract

▪ Abstract Practical limitations of the patch-clamp technique when recording mechanogated membrane ion channels are considered. Mechanical overstimulation of the patch or the cell from excessive suction/pressure protocols induces morphological and functional changes. In particular, the plasma membrane becomes decoupled from the underlying cytoskeleton to form either membrane blebs (cell-attached) or ghosts (whole cell). As a consequence, a membrane ion channel may show either a decrease or an increase in its native mechanosensitivity or even acquire mechanosensitivity. The effect varies with ion channel and cell type and presumably arises because of a disruption of membrane-cytoskeleton interactions. We consider that such disruptions are a pathological consequence of excessive mechanical stress, either during or after seal formation, rather than an immutable consequence of patch-clamp recording. By careful attention to the suction/pressure protocols during sealing and throughout recording, such artifacts can be avoided.

Bibliography

Hamill, O. P., & McBride, D. W. (1997). INDUCED MEMBRANE HYPO/HYPER-MECHANOSENSITIVITY:A Limitation of Patch-Clamp Recording. Annual Review of Physiology, 59(1), 621â631.

Authors 2

Owen P. Hamill (first)
Don W. McBride (additional)

References 51 Referenced 113

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Dates

Type	When
Created	23 years, 1 month ago (July 27, 2002, 7:40 a.m.)
Deposited	1 year, 7 months ago (Jan. 5, 2024, 11:44 p.m.)
Indexed	2 months ago (July 2, 2025, 5:17 a.m.)
Issued	27 years, 11 months ago (Oct. 1, 1997)
Published	27 years, 11 months ago (Oct. 1, 1997)
Published Print	27 years, 11 months ago (Oct. 1, 1997)

Funders 0

None

BibTeX

@article{Hamill_1997, title={INDUCED MEMBRANE HYPO/HYPER-MECHANOSENSITIVITY:A Limitation of Patch-Clamp Recording}, volume={59}, ISSN={1545-1585}, url={http://dx.doi.org/10.1146/annurev.physiol.59.1.621}, DOI={10.1146/annurev.physiol.59.1.621}, number={1}, journal={Annual Review of Physiology}, publisher={Annual Reviews}, author={Hamill, Owen P. and McBride, Don W.}, year={1997}, month=oct, pages={621–631} }

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