DOI: 10.1146/annurev.genet.32.1.163. THE GENETICS OF DISULFIDE BOND METABOLISM

THE GENETICS OF DISULFIDE BOND METABOLISM

10.1146/annurev.genet.32.1.163

Crossref journal-article

Annual Reviews

Annual Review of Genetics (22)

Abstract

▪ Abstract Disulfide bonds are required for the stability and function of a large number of proteins. Genetic analysis in combination with biochemical studies have elucidated the main catalysts involved in facilitating these processes in the cell. All enzymes involved in thiol-disulfide metabolism have a conserved active site that consists of two cysteine residues, separated by two intervening amino acids, the Cys-Xaa-Xaa-Cys motif. While these enzymes are capable of catalyzing both disulfide bond formation and reduction, they have evolved to perform one or the other reaction more efficiently. In the cytoplasm, multiple pathways are involved in the reduction of disulfide bonds that occur as part of the catalytic cycle of a variety of metabolic enzymes. In the bacterial periplasm, a system for the efficient introduction as well as isomerization of disulfide bonds is in place. In eukaryotes, disulfide bonds are introduced into proteins in the endoplasmic reticulum. Genetic studies have recently begun to reveal new features of this process. While the enzyme mechanisms of thiol-disulfide oxidoreductases have been the subject of much scrutiny, questions remain regarding where and when they act in vivo, their specificities, and the maintenance of the redox environment that determines their function.

Bibliography

Rietsch, A., & Beckwith, J. (1998). THE GENETICS OF DISULFIDE BOND METABOLISM. Annual Review of Genetics, 32(1), 163â184.

Authors 2

Arne Rietsch (first)
Jonathan Beckwith (additional)

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Dates

Type	When
Created	23 years ago (July 27, 2002, 7:43 a.m.)
Deposited	3 years, 10 months ago (Oct. 12, 2021, 8:05 a.m.)
Indexed	6 hours, 22 minutes ago (Aug. 26, 2025, 3:03 a.m.)
Issued	26 years, 8 months ago (Dec. 1, 1998)
Published	26 years, 8 months ago (Dec. 1, 1998)
Published Print	26 years, 8 months ago (Dec. 1, 1998)

Funders 0

None

BibTeX

@article{Rietsch_1998, title={THE GENETICS OF DISULFIDE BOND METABOLISM}, volume={32}, ISSN={1545-2948}, url={http://dx.doi.org/10.1146/annurev.genet.32.1.163}, DOI={10.1146/annurev.genet.32.1.163}, number={1}, journal={Annual Review of Genetics}, publisher={Annual Reviews}, author={Rietsch, Arne and Beckwith, Jonathan}, year={1998}, month=dec, pages={163–184} }

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