DOI: 10.1146/annurev.biophys.30.1.67. Mass Spectrometry as a Tool for Protein Crystallography

Mass Spectrometry as a Tool for Protein Crystallography

10.1146/annurev.biophys.30.1.67

Crossref journal-article

Annual Reviews

Annual Review of Biophysics and Biomolecular Structure (22)

Abstract

▪ Abstract Atomic resolution structure determinations of proteins by X-ray crystallography are formidable multidisciplinary undertakings, requiring protein construct design, expression and purification, crystallization trials, phase determination, and model building. Modern mass spectrometric methods can greatly facilitate these obligate tasks. Thus, mass spectrometry can be used to verify that the desired protein construct has been correctly expressed, to define compact domains in the target protein, to assess the components contained within the protein crystals, and to screen for successful incorporation of seleno-methionine and other heavy metal reagents used for phasing. In addition, mass spectrometry can be used to address issues of modeling, topology, and side-chain proximity. Here, we demonstrate how rational use of mass spectrometry assists and expedites high resolution X-ray structure determination through each stage of the process of protein crystallography.

Bibliography

Cohen, S. L., & Chait, B. T. (2001). Mass Spectrometry as a Tool for Protein Crystallography. Annual Review of Biophysics and Biomolecular Structure, 30(1), 67â85.

Authors 2

Steven L. Cohen (first)
Brian T. Chait (additional)

References 51 Referenced 49

Dates

Type	When
Created	23 years, 1 month ago (July 27, 2002, 7:49 a.m.)
Deposited	1 year, 7 months ago (Jan. 6, 2024, 12:57 a.m.)
Indexed	11 months, 2 weeks ago (Sept. 14, 2024, 7:13 a.m.)
Issued	24 years, 2 months ago (June 1, 2001)
Published	24 years, 2 months ago (June 1, 2001)
Published Print	24 years, 2 months ago (June 1, 2001)

Funders 0

None

BibTeX

@article{Cohen_2001, title={Mass Spectrometry as a Tool for Protein Crystallography}, volume={30}, ISSN={1545-4266}, url={http://dx.doi.org/10.1146/annurev.biophys.30.1.67}, DOI={10.1146/annurev.biophys.30.1.67}, number={1}, journal={Annual Review of Biophysics and Biomolecular Structure}, publisher={Annual Reviews}, author={Cohen, Steven L. and Chait, Brian T.}, year={2001}, month=jun, pages={67–85} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2024,
        9,
        14
      ]
    ],
    "date-time": "2024-09-14T11:13:00Z",
    "timestamp": 1726312380780
  },
  "reference-count": 51,
  "publisher": "Annual Reviews",
  "issue": "1",
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        2001,
        6
      ]
    ]
  },
  "abstract": "<jats:p>\u25aa Abstract\u2002 Atomic resolution structure determinations of proteins by X-ray crystallography are formidable multidisciplinary undertakings, requiring protein construct design, expression and purification, crystallization trials, phase determination, and model building. Modern mass spectrometric methods can greatly facilitate these obligate tasks. Thus, mass spectrometry can be used to verify that the desired protein construct has been correctly expressed, to define compact domains in the target protein, to assess the components contained within the protein crystals, and to screen for successful incorporation of seleno-methionine and other heavy metal reagents used for phasing. In addition, mass spectrometry can be used to address issues of modeling, topology, and side-chain proximity. Here, we demonstrate how rational use of mass spectrometry assists and expedites high resolution X-ray structure determination through each stage of the process of protein crystallography.</jats:p>",
  "DOI": "10.1146/annurev.biophys.30.1.67",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2002,
        7,
        27
      ]
    ],
    "date-time": "2002-07-27T11:49:00Z",
    "timestamp": 1027770540000
  },
  "page": "67-85",
  "source": "Crossref",
  "is-referenced-by-count": 49,
  "title": "Mass Spectrometry as a Tool for Protein Crystallography",
  "prefix": "10.1146",
  "volume": "30",
  "author": [
    {
      "given": "Steven L.",
      "family": "Cohen",
      "sequence": "first",
      "affiliation": [
        {
          "name": "Laboratory for Mass Spectrometry and Gaseous Ion Chemistry, Rockefeller University, New York, NY 10021;,"
        }
      ]
    },
    {
      "given": "Brian T.",
      "family": "Chait",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Laboratory for Mass Spectrometry and Gaseous Ion Chemistry, Rockefeller University, New York, NY 10021;,"
        }
      ]
    }
  ],
  "member": "22",
  "reference": [
    {
      "key": "b1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/27692"
    },
    {
      "key": "b2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/bi990781k"
    },
    {
      "key": "b3",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0076-6879(96)70024-1"
    },
    {
      "key": "b4",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0969-2126(00)00168-4"
    },
    {
      "key": "b5",
      "doi-asserted-by": "publisher",
      "DOI": "10.1006/jmbi.1997.1132"
    },
    {
      "key": "b6",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1432-1033.1995.tb20622.x"
    },
    {
      "key": "b7",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0959-440X(97)80012-7"
    },
    {
      "key": "b8",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/ac000811l"
    },
    {
      "key": "b9",
      "doi-asserted-by": "crossref",
      "first-page": "7304",
      "DOI": "10.1016/S0021-9258(17)37284-8",
      "volume": "269",
      "author": "Carmel G",
      "year": "1994",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "b10",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0969-2126(00)00047-2"
    },
    {
      "key": "b11",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/pro.5560071216"
    },
    {
      "key": "b12",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(00)81443-9"
    },
    {
      "key": "b13",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0969-2126(96)00108-6"
    },
    {
      "key": "b14",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/pro.5560040607"
    },
    {
      "key": "b15",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/ac990938e"
    },
    {
      "key": "b16",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0076-6879(97)76075-0"
    },
    {
      "key": "b17",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.280.5360.69"
    },
    {
      "key": "b18",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/pro.5560070231"
    },
    {
      "key": "b19",
      "doi-asserted-by": "publisher",
      "DOI": "10.1006/jmbi.2000.3748"
    },
    {
      "key": "b20",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1432-1033.1993.tb17589.x"
    },
    {
      "key": "b21",
      "doi-asserted-by": "publisher",
      "DOI": "10.1046/j.1432-1327.1998.2580831.x"
    },
    {
      "key": "b22",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.1925561"
    },
    {
      "key": "b23",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/ac00013a003"
    },
    {
      "key": "b24",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0896-6273(00)80900-9"
    },
    {
      "key": "b25",
      "doi-asserted-by": "publisher",
      "DOI": "10.1107/S090744499901121X"
    },
    {
      "key": "b26",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(00)81276-3"
    },
    {
      "key": "b27",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.275.18.13683"
    },
    {
      "key": "b28",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0969-2126(99)80025-2"
    },
    {
      "key": "b29",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(00)80668-6"
    },
    {
      "key": "b30",
      "doi-asserted-by": "crossref",
      "first-page": "165",
      "DOI": "10.1111/j.1470-8744.1999.tb00546.x",
      "volume": "29",
      "author": "Li X",
      "year": "1999",
      "journal-title": "Biotechnol. Appl. Biochem."
    },
    {
      "key": "b31",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(00)81329-X"
    },
    {
      "key": "b32",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0968-0004(00)89019-2"
    },
    {
      "key": "b33",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(00)80280-9"
    },
    {
      "key": "b34",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/bi00087a027"
    },
    {
      "key": "b35",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/bi00449a034"
    },
    {
      "key": "b36",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(00)81635-9"
    },
    {
      "key": "b37",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/nsb0994-621"
    },
    {
      "key": "b38",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/377119a0"
    },
    {
      "key": "b39",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/protein/12.5.371"
    },
    {
      "key": "b40",
      "doi-asserted-by": "publisher",
      "DOI": "10.1023/A:1026327125333"
    },
    {
      "key": "b41",
      "doi-asserted-by": "publisher",
      "DOI": "10.1006/jmbi.1996.0604"
    },
    {
      "key": "b42",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0959-440X(95)80063-8"
    },
    {
      "key": "b43",
      "doi-asserted-by": "publisher",
      "DOI": "10.1107/S0907444999015188"
    },
    {
      "key": "b44",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/j.1460-2075.1996.tb00946.x"
    },
    {
      "key": "b45",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0065-3233(00)54001-7"
    },
    {
      "key": "b46",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/380316a0"
    },
    {
      "key": "b47",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/j.1460-2075.1995.tb07082.x"
    },
    {
      "key": "b48",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.090099097"
    },
    {
      "key": "b49",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/pro.5560060417"
    },
    {
      "key": "b50",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/386247a0"
    },
    {
      "key": "b51",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.271.49.31360"
    }
  ],
  "container-title": "Annual Review of Biophysics and Biomolecular Structure",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://www.annualreviews.org/doi/pdf/10.1146/annurev.biophys.30.1.67",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2024,
        1,
        6
      ]
    ],
    "date-time": "2024-01-06T05:57:00Z",
    "timestamp": 1704520620000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://www.annualreviews.org/doi/10.1146/annurev.biophys.30.1.67"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        2001,
        6
      ]
    ]
  },
  "references-count": 51,
  "journal-issue": {
    "issue": "1",
    "published-print": {
      "date-parts": [
        [
          2001,
          6
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1146/annurev.biophys.30.1.67"
  ],
  "URL": "http://dx.doi.org/10.1146/annurev.biophys.30.1.67",
  "relation": {},
  "ISSN": [
    "1056-8700",
    "1545-4266"
  ],
  "subject": [],
  "container-title-short": "Annu. Rev. Biophys. Biomol. Struct.",
  "published": {
    "date-parts": [
      [
        2001,
        6
      ]
    ]
  }
}