DOI: 10.1146/annurev.biochem.77.061906.092014. Super-Resolution Fluorescence Microscopy

Super-Resolution Fluorescence Microscopy

10.1146/annurev.biochem.77.061906.092014

Crossref journal-article

Annual Reviews

Annual Review of Biochemistry (22)

Abstract

Achieving a spatial resolution that is not limited by the diffraction of light, recent developments of super-resolution fluorescence microscopy techniques allow the observation of many biological structures not resolvable in conventional fluorescence microscopy. New advances in these techniques now give them the ability to image three-dimensional (3D) structures, measure interactions by multicolor colocalization, and record dynamic processes in living cells at the nanometer scale. It is anticipated that super-resolution fluorescence microscopy will become a widely used tool for cell and tissue imaging to provide previously unobserved details of biological structures and processes.

Bibliography

Huang, B., Bates, M., & Zhuang, X. (2009). Super-Resolution Fluorescence Microscopy. Annual Review of Biochemistry, 78(1), 993â1016.

Authors 3

Bo Huang (first)
Mark Bates (additional)
Xiaowei Zhuang (additional)

References 96 Referenced 1,438

10.1364/OL.19.000780
10.1364/OL.24.000954
10.1126/science.1137395
10.1073/pnas.0406877102
10.1038/nmeth929
10.1126/science.1127344
10.1529/biophysj.106.091116
10.1016/S0006-3495(02)75618-X
10.1063/1.94865
10.1126/science.251.5000.1468
10.1017/CBO9780511813535
10.1126/science.1138746
10.1126/science.1137368
10.1007/978-0-387-45524-2
10.1038/nbt899
10.1016/0030-4018(92)90185-T
10.1046/j.1365-2818.1999.00576.x
10.1038/366044a0
10.1046/j.1365-2818.2000.00710.x
10.1529/biophysj.107.120345
10.1126/science.1156947
10.1529/biophysj.107.120352
10.1002/anie.200802376
10.1103/PhysRevLett.94.143903
10.1103/PhysRevLett.88.163901
10.1073/pnas.0604965103
10.1038/nmeth922
10.1007/BF01081333
10.1103/PhysRevLett.98.218103
10.1073/pnas.0506010102
10.1038/331450a0
10.1016/S0006-3495(94)80939-7
10.1038/nature04268
10.1103/PhysRevLett.62.2535
10.1103/PhysRevLett.65.2716
10.1126/science.1084398
10.1016/S0009-2614(98)00673-3
10.1073/pnas.170286097
10.1073/pnas.0409487102
10.1073/pnas.0401638101
10.1073/pnas.0402155101
10.1364/OPEX.13.007052
10.1529/biophysj.105.079178
10.1529/biophysj.107.112201
10.1073/pnas.0609643104
10.1126/science.1146598
10.1016/S0006-3495(94)80601-0
10.1002/(SICI)1438-5171(200004)1:1<25::AID-SIMO25>3.3.CO;2-F
10.1063/1.2437066
10.1021/nl0709120
10.1016/j.bbrc.2007.04.168
10.1126/science.1153529
10.1038/nmeth.1274
10.1038/nmeth.1211
10.1021/nl073164n
10.1038/nmeth.1214
10.1529/biophysj.107.104497
10.1103/PhysRevLett.94.108101
10.1021/nl801471d
10.1073/pnas.0710517105
10.1038/nbt.1493
10.1038/nmeth.1298
10.1088/1367-2630/9/12/435
10.1126/science.1154228
10.1038/nmeth.1202
10.1038/nmeth.1258
10.1073/pnas.0708066104
10.1038/nmeth.1176
10.1364/OE.14.008111
{'key': 'B70', 'first-page': '10', 'volume': '37', 'author': 'Shannon CE', 'year': '1949', 'journal-title': 'Proc. Inst. Radio Eng.'} / Proc. Inst. Radio Eng. by Shannon CE (1949)
10.1073/pnas.75.4.1820
10.1016/j.copbio.2004.12.003
10.1152/physiol.00007.2008
10.1126/science.281.5374.269
10.1038/nbt954
10.1038/nmeth735
10.1038/nbt1355
10.1038/nchembio.2007.31
10.1126/science.287.5460.2007
{'key': 'B80', 'volume-title': 'Cellular Electron Microscopy', 'volume': '79', 'author': 'McIntosh JR', 'year': '2007'} / Cellular Electron Microscopy by McIntosh JR (2007)
10.1002/jemt.20443
10.1007/s00339-007-4144-0
10.1038/nmeth1108
10.1038/nature04592
10.1126/science.1126308
10.1529/biophysj.105.079574
10.1126/science.1141727
10.1126/science.1074952
10.1038/nbt1025
10.1073/pnas.202320599
10.1073/pnas.0403668101
10.1038/nbt1191
10.1126/science.1102506
10.1021/ja074704l
10.1042/BJ20061401
10.1002/anie.200702167

Dates

Type	When
Created	16 years, 3 months ago (June 2, 2009, 3:37 p.m.)
Deposited	3 years, 10 months ago (Oct. 5, 2021, 8:05 a.m.)
Indexed	2 days, 11 hours ago (Aug. 31, 2025, 7:22 p.m.)
Issued	16 years, 3 months ago (June 1, 2009)
Published	16 years, 3 months ago (June 1, 2009)
Published Print	16 years, 3 months ago (June 1, 2009)

Funders 0

None

BibTeX

@article{Huang_2009, title={Super-Resolution Fluorescence Microscopy}, volume={78}, ISSN={1545-4509}, url={http://dx.doi.org/10.1146/annurev.biochem.77.061906.092014}, DOI={10.1146/annurev.biochem.77.061906.092014}, number={1}, journal={Annual Review of Biochemistry}, publisher={Annual Reviews}, author={Huang, Bo and Bates, Mark and Zhuang, Xiaowei}, year={2009}, month=jun, pages={993–1016} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        8,
        31
      ]
    ],
    "date-time": "2025-08-31T23:22:28Z",
    "timestamp": 1756682548854
  },
  "reference-count": 96,
  "publisher": "Annual Reviews",
  "issue": "1",
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        2009,
        6,
        1
      ]
    ]
  },
  "abstract": "<jats:p> Achieving a spatial resolution that is not limited by the diffraction of light, recent developments of super-resolution fluorescence microscopy techniques allow the observation of many biological structures not resolvable in conventional fluorescence microscopy. New advances in these techniques now give them the ability to image three-dimensional (3D) structures, measure interactions by multicolor colocalization, and record dynamic processes in living cells at the nanometer scale. It is anticipated that super-resolution fluorescence microscopy will become a widely used tool for cell and tissue imaging to provide previously unobserved details of biological structures and processes. </jats:p>",
  "DOI": "10.1146/annurev.biochem.77.061906.092014",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2009,
        6,
        2
      ]
    ],
    "date-time": "2009-06-02T19:37:16Z",
    "timestamp": 1243971436000
  },
  "page": "993-1016",
  "source": "Crossref",
  "is-referenced-by-count": 1438,
  "title": "Super-Resolution Fluorescence Microscopy",
  "prefix": "10.1146",
  "volume": "78",
  "author": [
    {
      "given": "Bo",
      "family": "Huang",
      "sequence": "first",
      "affiliation": [
        {
          "name": "Howard Hughes Medical Institute, Harvard University, Cambridge, Massachusetts 02138;"
        },
        {
          "name": "Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138;"
        }
      ]
    },
    {
      "given": "Mark",
      "family": "Bates",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "School of Engineering and Applied Science, Harvard University, Cambridge, Massachusetts 02138;"
        }
      ]
    },
    {
      "given": "Xiaowei",
      "family": "Zhuang",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Howard Hughes Medical Institute, Harvard University, Cambridge, Massachusetts 02138;"
        },
        {
          "name": "Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138;"
        },
        {
          "name": "Department of Physics, Harvard University, Cambridge, Massachusetts 02138;"
        }
      ]
    }
  ],
  "member": "22",
  "reference": [
    {
      "key": "B1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1364/OL.19.000780"
    },
    {
      "key": "B2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1364/OL.24.000954"
    },
    {
      "key": "B3",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.1137395"
    },
    {
      "key": "B4",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.0406877102"
    },
    {
      "key": "B5",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/nmeth929"
    },
    {
      "key": "B6",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.1127344"
    },
    {
      "key": "B7",
      "doi-asserted-by": "publisher",
      "DOI": "10.1529/biophysj.106.091116"
    },
    {
      "key": "B8",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0006-3495(02)75618-X"
    },
    {
      "key": "B9",
      "doi-asserted-by": "publisher",
      "DOI": "10.1063/1.94865"
    },
    {
      "key": "B10",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.251.5000.1468"
    },
    {
      "key": "B11",
      "doi-asserted-by": "publisher",
      "DOI": "10.1017/CBO9780511813535"
    },
    {
      "key": "B12",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.1138746"
    },
    {
      "key": "B13",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.1137368"
    },
    {
      "key": "B14",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/978-0-387-45524-2"
    },
    {
      "key": "B15",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/nbt899"
    },
    {
      "key": "B16",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0030-4018(92)90185-T"
    },
    {
      "key": "B17",
      "doi-asserted-by": "publisher",
      "DOI": "10.1046/j.1365-2818.1999.00576.x"
    },
    {
      "key": "B18",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/366044a0"
    },
    {
      "key": "B19",
      "doi-asserted-by": "publisher",
      "DOI": "10.1046/j.1365-2818.2000.00710.x"
    },
    {
      "key": "B20",
      "doi-asserted-by": "publisher",
      "DOI": "10.1529/biophysj.107.120345"
    },
    {
      "key": "B21",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.1156947"
    },
    {
      "key": "B22",
      "doi-asserted-by": "publisher",
      "DOI": "10.1529/biophysj.107.120352"
    },
    {
      "key": "B23",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/anie.200802376"
    },
    {
      "key": "B24",
      "doi-asserted-by": "publisher",
      "DOI": "10.1103/PhysRevLett.94.143903"
    },
    {
      "key": "B25",
      "doi-asserted-by": "publisher",
      "DOI": "10.1103/PhysRevLett.88.163901"
    },
    {
      "key": "B26",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.0604965103"
    },
    {
      "key": "B27",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/nmeth922"
    },
    {
      "key": "B28",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF01081333"
    },
    {
      "key": "B29",
      "doi-asserted-by": "publisher",
      "DOI": "10.1103/PhysRevLett.98.218103"
    },
    {
      "key": "B30",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.0506010102"
    },
    {
      "key": "B31",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/331450a0"
    },
    {
      "key": "B32",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0006-3495(94)80939-7"
    },
    {
      "key": "B33",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/nature04268"
    },
    {
      "key": "B34",
      "doi-asserted-by": "publisher",
      "DOI": "10.1103/PhysRevLett.62.2535"
    },
    {
      "key": "B35",
      "doi-asserted-by": "publisher",
      "DOI": "10.1103/PhysRevLett.65.2716"
    },
    {
      "key": "B36",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.1084398"
    },
    {
      "key": "B37",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0009-2614(98)00673-3"
    },
    {
      "key": "B38",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.170286097"
    },
    {
      "key": "B39",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.0409487102"
    },
    {
      "key": "B40",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.0401638101"
    },
    {
      "key": "B41",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.0402155101"
    },
    {
      "key": "B42",
      "doi-asserted-by": "publisher",
      "DOI": "10.1364/OPEX.13.007052"
    },
    {
      "key": "B43",
      "doi-asserted-by": "publisher",
      "DOI": "10.1529/biophysj.105.079178"
    },
    {
      "key": "B44",
      "doi-asserted-by": "publisher",
      "DOI": "10.1529/biophysj.107.112201"
    },
    {
      "key": "B45",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.0609643104"
    },
    {
      "key": "B46",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.1146598"
    },
    {
      "key": "B47",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0006-3495(94)80601-0"
    },
    {
      "key": "B48",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/(SICI)1438-5171(200004)1:1<25::AID-SIMO25>3.3.CO;2-F"
    },
    {
      "key": "B49",
      "doi-asserted-by": "publisher",
      "DOI": "10.1063/1.2437066"
    },
    {
      "key": "B50",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/nl0709120"
    },
    {
      "key": "B51",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.bbrc.2007.04.168"
    },
    {
      "key": "B52",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.1153529"
    },
    {
      "key": "B53",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/nmeth.1274"
    },
    {
      "key": "B54",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/nmeth.1211"
    },
    {
      "key": "B55",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/nl073164n"
    },
    {
      "key": "B56",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/nmeth.1214"
    },
    {
      "key": "B57",
      "doi-asserted-by": "publisher",
      "DOI": "10.1529/biophysj.107.104497"
    },
    {
      "key": "B58",
      "doi-asserted-by": "publisher",
      "DOI": "10.1103/PhysRevLett.94.108101"
    },
    {
      "key": "B59",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/nl801471d"
    },
    {
      "key": "B60",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.0710517105"
    },
    {
      "key": "B61",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/nbt.1493"
    },
    {
      "key": "B62",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/nmeth.1298"
    },
    {
      "key": "B63",
      "doi-asserted-by": "publisher",
      "DOI": "10.1088/1367-2630/9/12/435"
    },
    {
      "key": "B64",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.1154228"
    },
    {
      "key": "B65",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/nmeth.1202"
    },
    {
      "key": "B66",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/nmeth.1258"
    },
    {
      "key": "B67",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.0708066104"
    },
    {
      "key": "B68",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/nmeth.1176"
    },
    {
      "key": "B69",
      "doi-asserted-by": "publisher",
      "DOI": "10.1364/OE.14.008111"
    },
    {
      "key": "B70",
      "first-page": "10",
      "volume": "37",
      "author": "Shannon CE",
      "year": "1949",
      "journal-title": "Proc. Inst. Radio Eng."
    },
    {
      "key": "B71",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.75.4.1820"
    },
    {
      "key": "B72",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.copbio.2004.12.003"
    },
    {
      "key": "B73",
      "doi-asserted-by": "publisher",
      "DOI": "10.1152/physiol.00007.2008"
    },
    {
      "key": "B74",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.281.5374.269"
    },
    {
      "key": "B75",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/nbt954"
    },
    {
      "key": "B76",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/nmeth735"
    },
    {
      "key": "B77",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/nbt1355"
    },
    {
      "key": "B78",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/nchembio.2007.31"
    },
    {
      "key": "B79",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.287.5460.2007"
    },
    {
      "key": "B80",
      "volume-title": "Cellular Electron Microscopy",
      "volume": "79",
      "author": "McIntosh JR",
      "year": "2007"
    },
    {
      "key": "B81",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/jemt.20443"
    },
    {
      "key": "B82",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/s00339-007-4144-0"
    },
    {
      "key": "B83",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/nmeth1108"
    },
    {
      "key": "B84",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/nature04592"
    },
    {
      "key": "B85",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.1126308"
    },
    {
      "key": "B86",
      "doi-asserted-by": "publisher",
      "DOI": "10.1529/biophysj.105.079574"
    },
    {
      "key": "B87",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.1141727"
    },
    {
      "key": "B88",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.1074952"
    },
    {
      "key": "B89",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/nbt1025"
    },
    {
      "key": "B90",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.202320599"
    },
    {
      "key": "B91",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.0403668101"
    },
    {
      "key": "B92",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/nbt1191"
    },
    {
      "key": "B93",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.1102506"
    },
    {
      "key": "B94",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/ja074704l"
    },
    {
      "key": "B95",
      "doi-asserted-by": "publisher",
      "DOI": "10.1042/BJ20061401"
    },
    {
      "key": "B96",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/anie.200702167"
    }
  ],
  "container-title": "Annual Review of Biochemistry",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://www.annualreviews.org/doi/pdf/10.1146/annurev.biochem.77.061906.092014",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2021,
        10,
        5
      ]
    ],
    "date-time": "2021-10-05T12:05:52Z",
    "timestamp": 1633435552000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://www.annualreviews.org/doi/10.1146/annurev.biochem.77.061906.092014"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        2009,
        6,
        1
      ]
    ]
  },
  "references-count": 96,
  "journal-issue": {
    "issue": "1",
    "published-print": {
      "date-parts": [
        [
          2009,
          6,
          1
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1146/annurev.biochem.77.061906.092014"
  ],
  "URL": "http://dx.doi.org/10.1146/annurev.biochem.77.061906.092014",
  "relation": {},
  "ISSN": [
    "0066-4154",
    "1545-4509"
  ],
  "subject": [],
  "container-title-short": "Annu. Rev. Biochem.",
  "published": {
    "date-parts": [
      [
        2009,
        6,
        1
      ]
    ]
  }
}