DOI: 10.1146/annurev.biochem.76.061005.090816. The Role of Mass Spectrometry in Structure Elucidation of Dynamic Protein Complexes

The Role of Mass Spectrometry in Structure Elucidation of Dynamic Protein Complexes

10.1146/annurev.biochem.76.061005.090816

Crossref journal-article

Annual Reviews

Annual Review of Biochemistry (22)

Abstract

The fact that ions of macromolecular complexes produced by electrospray ionization can be maintained intact in a mass spectrometer has stimulated exciting new lines of research. In this review we chart the progress of this research from the observation of simple homo-oligomers to complex heterogeneous macromolecular assemblies of mega-Dalton proportions. The applications described herein not only confirm the status of mass spectrometry (MS) as a structural biology approach to complement X-ray analysis or electron microscopy, but also highlight unique attributes of the methodology. This is exemplified in studies of the biogenesis of macromolecular complexes and in the exchange of subunits between macromolecular complexes. Moreover, recent successes in revealing the overall subunit architecture of complexes are set to promote MS from a complementary approach to a structural biology tool in its own right.

Bibliography

Sharon, M., & Robinson, C. V. (2007). The Role of Mass Spectrometry in Structure Elucidation of Dynamic Protein Complexes. Annual Review of Biochemistry, 76(1), 167â193.

Authors 2

Michal Sharon (first)
Carol V. Robinson (additional)

References 127 Referenced 326

10.1126/science.2675315
10.1002/rcm.1290020802
10.1021/ac00171a028
10.1146/annurev.biochem.70.1.437
10.1038/nrm1711
10.1126/science.1124619
10.1002/(SICI)1098-2787(1997)16:1<1::AID-MAS1>3.0.CO;2-L
10.1074/jbc.R100024200
10.1016/S0959-440X(02)00400-1
10.1074/jbc.R300037200
10.1016/j.cbpa.2004.08.006
10.1098/rsta.2004.1498
10.1016/j.sbi.2006.03.009
10.1038/415141a
10.1038/35001009
10.1016/S1044-0305(97)00246-8
10.1021/ac0110552
10.1038/sj.embor.7400702 / EMBO Rep. by Hernández H (2006)
10.1016/j.chembiol.2006.04.006
10.1021/ja061468q
10.1002/rcm.2620
10.1021/ja993740k
10.1002/1521-3773(20010316)40:6<9822::AID-ANIE9822>3.3.CO;2-I
10.1073/pnas.97.10.5185
10.1074/jbc.M208966200
{'key': 'B26', 'volume-title': 'Electrospray Ionization Mass Spectrometry: Fundamentals, Instrumentation and Applications', 'author': 'Cole RB', 'year': '1997'} / Electrospray Ionization Mass Spectrometry: Fundamentals, Instrumentation and Applications by Cole RB (1997)
10.1002/(SICI)1096-9888(199707)32:7<677::AID-JMS536>3.3.CO;2-7
10.1016/0168-1176(94)04024-9
10.1021/ac9509519
10.1110/ps.8.10.1962
10.1016/j.jasms.2005.06.011
10.1063/1.1670391
10.1063/1.432536
10.1016/S0003-2670(99)00599-1
10.1002/1096-9888(200007)35:7<763::AID-JMS16>3.0.CO;2-#
10.1002/1096-9888(200007)35:7<804::AID-JMS22>3.0.CO;2-Q
10.1016/j.ijms.2003.10.009
10.1021/ac050511+
10.1021/ac035406j
10.1021/ja0317170
10.1016/S0969-2126(04)00019-X
10.1073/pnas.0502193102
10.1021/ac0103975
10.1016/S1044-0305(02)00647-5
10.1021/ja0211508
10.1016/S0959-440X(99)80018-9
10.1016/j.ijms.2003.09.008
10.1016/1044-0305(95)00191-F
10.1016/j.chembiol.2006.04.006
10.1073/pnas.1932958100
10.1074/jbc.M511951200
10.1016/j.jasms.2004.06.006
10.1093/emboj/cdg359
10.1021/ja960211x
10.1073/pnas.240326597
10.1016/j.jasms.2005.02.017
10.1016/j.str.2006.05.019
10.1016/j.jmb.2003.08.029
10.1016/j.ijms.2004.05.010
10.1006/jmbi.1998.1937
10.1021/ac034251c
10.1021/ja990238r
10.1110/ps.9.3.435
10.1110/ps.11101
10.1073/pnas.061034498
10.1016/S1044-0305(00)00150-1
10.1016/S0006-3495(01)75981-4
10.1021/bm0101668
10.1042/BJ20030541
10.1002/jms.443
10.1074/jbc.M209052200
10.1016/j.jmb.2004.04.031
10.1110/ps.04889604
10.1042/BJ20050985
10.1074/jbc.M605270200
10.1006/abio.2001.5358
10.1074/jbc.M310958200
10.1016/j.molcel.2005.04.019
10.1038/sj.emboj.7600152
10.1074/jbc.M503189200
10.1529/biophysj.105.076455
10.1016/j.jmb.2005.11.085
10.1111/j.1742-4658.2006.05168.x
10.1016/j.jsb.2006.03.015
10.1042/BJ20020125
10.1074/jbc.271.12.7218
10.1074/jbc.M504263200
10.1073/pnas.1932958100
10.1074/jbc.M403348200
10.1074/jbc.M206060200
10.1074/jbc.M500135200
10.1074/jbc.M508753200
Deleted in proof
10.1016/j.molcel.2006.05.033
10.1021/bi048501a
10.1021/bi052349a
10.1016/S0092-8674(00)80922-8
10.1038/nmeth753
10.1016/j.ddtec.2006.03.008
10.1021/ja056756l
10.1371/journal.pbio.0040267
10.1016/j.febslet.2004.12.003
10.1074/jbc.M405718200
10.1073/pnas.96.20.11033
10.1126/science.282.5390.902
10.1016/S0092-8674(00)81515-9
10.1016/j.jmb.2003.11.004
10.1016/j.jmb.2006.02.012
10.1126/science.1092645
10.1093/embo-reports/kvf135
10.1074/mcp.M600043-MCP200
10.1002/3527602852
10.1002/jms.208
10.1155/2004/376572
10.1126/science.1120177
10.1002/rcm.1641
10.1016/j.ijms.2004.05.008
10.1002/pro.5560070512
10.1016/S0969-2126(02)00771-2
10.1126/science.1105932
10.1016/S0006-3495(03)74795-X
10.1021/ja0450307
10.1038/nature04532
10.1038/415180a
10.1038/13732
10.1093/nar/29.4.e24
10.1021/pr050225e

Dates

Type	When
Created	18 years, 6 months ago (Feb. 9, 2007, 12:34 p.m.)
Deposited	3 years, 10 months ago (Oct. 5, 2021, 4:33 p.m.)
Indexed	1 month, 2 weeks ago (July 2, 2025, 1:53 p.m.)
Issued	18 years, 2 months ago (June 7, 2007)
Published	18 years, 2 months ago (June 7, 2007)
Published Print	18 years, 2 months ago (June 7, 2007)

Funders 0

None

BibTeX

@article{Sharon_2007, title={The Role of Mass Spectrometry in Structure Elucidation of Dynamic Protein Complexes}, volume={76}, ISSN={1545-4509}, url={http://dx.doi.org/10.1146/annurev.biochem.76.061005.090816}, DOI={10.1146/annurev.biochem.76.061005.090816}, number={1}, journal={Annual Review of Biochemistry}, publisher={Annual Reviews}, author={Sharon, Michal and Robinson, Carol V.}, year={2007}, month=jun, pages={167–193} }

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