STRUCTURAL INSIGHTS INTO TRANSLATIONAL FIDELITY
10.1146/annurev.biochem.74.061903.155440
Crossref journal-article
Annual Reviews
Annual Review of Biochemistry (22)
Abstract

▪ Abstract  The underlying basis for the accuracy of protein synthesis has been the subject of over four decades of investigation. Recent biochemical and structural data make it possible to understand at least in outline the structural basis for tRNA selection, in which codon recognition by cognate tRNA results in the hydrolysis of GTP by EF-Tu over 75 Å away. The ribosome recognizes the geometry of codon-anticodon base pairing at the first two positions but monitors the third, or wobble position, less stringently. Part of the additional binding energy of cognate tRNA is used to induce conformational changes in the ribosome that stabilize a transition state for GTP hydrolysis by EF-Tu and subsequently result in accelerated accommodation of tRNA into the peptidyl transferase center. The transition state for GTP hydrolysis is characterized, among other things, by a distorted tRNA. This picture explains a large body of data on the effect of antibiotics and mutations on translational fidelity. However, many fundamental questions remain, such as the mechanism of activation of GTP hydrolysis by EF-Tu, and the relationship between decoding and frameshifting.

Bibliography

Ogle, J. M., & Ramakrishnan, V. (2005). STRUCTURAL INSIGHTS INTO TRANSLATIONAL FIDELITY. Annual Review of Biochemistry, 74(1), 129–177.

Authors 2
  1. James M. Ogle (first)
  2. V. Ramakrishnan (additional)
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Dates
Type When
Created 20 years, 5 months ago (Feb. 25, 2005, 10:55 p.m.)
Deposited 3 years, 10 months ago (Oct. 5, 2021, 7 a.m.)
Indexed 2 weeks ago (Aug. 7, 2025, 4:54 p.m.)
Issued 20 years, 2 months ago (June 1, 2005)
Published 20 years, 2 months ago (June 1, 2005)
Published Print 20 years, 2 months ago (June 1, 2005)
Funders 0

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@article{Ogle_2005, title={STRUCTURAL INSIGHTS INTO TRANSLATIONAL FIDELITY}, volume={74}, ISSN={1545-4509}, url={http://dx.doi.org/10.1146/annurev.biochem.74.061903.155440}, DOI={10.1146/annurev.biochem.74.061903.155440}, number={1}, journal={Annual Review of Biochemistry}, publisher={Annual Reviews}, author={Ogle, James M. and Ramakrishnan, V.}, year={2005}, month=jun, pages={129–177} }