THE GREEN FLUORESCENT PROTEIN
10.1146/annurev.biochem.67.1.509
Crossref journal-article
Annual Reviews
Annual Review of Biochemistry (22)
Abstract

In just three years, the green fluorescent protein (GFP) from the jellyfish Aequorea victoria has vaulted from obscurity to become one of the most widely studied and exploited proteins in biochemistry and cell biology. Its amazing ability to generate a highly visible, efficiently emitting internal fluorophore is both intrinsically fascinating and tremendously valuable. High-resolution crystal structures of GFP offer unprecedented opportunities to understand and manipulate the relation between protein structure and spectroscopic function. GFP has become well established as a marker of gene expression and protein targeting in intact cells and organisms. Mutagenesis and engineering of GFP into chimeric proteins are opening new vistas in physiological indicators, biosensors, and photochemical memories.

Bibliography

Tsien, R. Y. (1998). THE GREEN FLUORESCENT PROTEIN. Annual Review of Biochemistry, 67(1), 509–544.

Authors 1
  1. Roger Y. Tsien (first)
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Dates
Type When
Created 23 years ago (July 27, 2002, 7:42 a.m.)
Deposited 8 months, 2 weeks ago (Dec. 8, 2024, 5:57 p.m.)
Indexed 1 day, 12 hours ago (Aug. 21, 2025, 2:13 p.m.)
Issued 27 years, 2 months ago (June 1, 1998)
Published 27 years, 2 months ago (June 1, 1998)
Published Print 27 years, 2 months ago (June 1, 1998)
Funders 0

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@article{Tsien_1998, title={THE GREEN FLUORESCENT PROTEIN}, volume={67}, ISSN={1545-4509}, url={http://dx.doi.org/10.1146/annurev.biochem.67.1.509}, DOI={10.1146/annurev.biochem.67.1.509}, number={1}, journal={Annual Review of Biochemistry}, publisher={Annual Reviews}, author={Tsien, Roger Y.}, year={1998}, month=jun, pages={509–544} }